DOI: 10.4049/jimmunol.138.5.1457. UV-irradiated epidermal cells produce a specific inhibitor of interleukin 1 activity.

UV-irradiated epidermal cells produce a specific inhibitor of interleukin 1 activity.

10.4049/jimmunol.138.5.1457

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract UV irradiation of epidermal cells (EC) in vitro and in vivo leads to an enhanced synthesis of the immunostimulating cytokine interleukin 1 (IL 1). However, UV exposure in vivo also results in local as well as systemic immunosuppression. Therefore, it was tested whether UV-exposed murine EC in culture in addition to IL 1 release an inhibitor of IL 1 activity. Supernatants of UV-irradiated BALB/c EC and of a transformed keratinocyte cell line (Pam 212) were evaluated for their ability to suppress IL 1-mediated thymocyte proliferation. Crude supernatants derived from either UV-exposed or unirradiated EC did not interfere with IL 1 activity. When supernatants were subjected to HPLC gel filtration, fractions eluting at approximately 40 kD significantly blocked the activity of EC-derived IL 1 and murine recombinant IL 1. The release of this inhibitory cytokine (EC-derived contra-IL 1 [EC-contra-IL 1]) was confined to UV-exposed BALB/c or Pam 212 keratinocytes, since no inhibitory activity was detected in supernatants of unirradiated cells. EC-contra-IL 1 also blocked IL 1-induced fibroblast proliferation but did not suppress IL 2 or IL 3 activity. Moreover, EC-contra-IL 1 did not inhibit spontaneous proliferation of a variety of cell lines (Pam 212, P388D1, L 929, EL 4). With the use of chromatofocusing EC-contra-IL 1 exhibited a pI of 8.8, and upon reversed-phase chromatography it eluted within three distinct peaks. Therefore, murine UV-exposed EC, in addition to the production of immunoenhancing cytokines, also may release immunosuppressing mediators and thereby participate in UV-induced immunosuppression. These findings further support the notion that the epidermis may not only be considered as a simple barrier against harmful agents but represents an active element of the immune system.

Bibliography

Schwarz, T., Urbanska, A., Gschnait, F., & Luger, T. A. (1987). UV-irradiated epidermal cells produce a specific inhibitor of interleukin 1 activity. The Journal of Immunology, 138(5), 1457â1463.

Authors 4

T Schwarz (first)
A Urbanska (additional)
F Gschnait (additional)
T A Luger (additional)

References 0 Referenced 91

None

Dates

Type	When
Created	2 years, 7 months ago (Dec. 31, 2022, 5 p.m.)
Deposited	4 months, 4 weeks ago (March 31, 2025, 4:47 p.m.)
Indexed	4 months, 3 weeks ago (April 1, 2025, 12:12 a.m.)
Issued	38 years, 5 months ago (March 1, 1987)
Published	38 years, 5 months ago (March 1, 1987)
Published Online	38 years, 5 months ago (March 1, 1987)
Published Print	38 years, 5 months ago (March 1, 1987)

Funders 0

None

BibTeX

@article{Schwarz_1987, title={UV-irradiated epidermal cells produce a specific inhibitor of interleukin 1 activity.}, volume={138}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.138.5.1457}, DOI={10.4049/jimmunol.138.5.1457}, number={5}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Schwarz, T and Urbanska, A and Gschnait, F and Luger, T A}, year={1987}, month=mar, pages={1457–1463} }

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