DOI: 10.4049/jimmunol.133.1.334. Association of Rho(D) polypeptides with the membrane skeleton in Rho(D)-positive human red cells.

Association of Rho(D) polypeptides with the membrane skeleton in Rho(D)-positive human red cells.

10.4049/jimmunol.133.1.334

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The Rho(D) antigen was recently identified as a 28,000 to 33,000 m.w. polypeptide expressed on the surface of human Rho(D)+ cells. We now show that 70 to 80% of the Rho(D) polypeptides remain firmly associated with the membrane skeleton (detergent-insoluble matrix) obtained after treatment of isolated membranes with Triton X-100. The same treatment solubilized most of the major sialoglycoprotein, glycophorin A. The membrane skeleton-bound Rho(D) polypeptides were not solubilized by procedures that dissociated spectrin, actin, and glyceraldehyde-3-phosphate dehydrogenase from the membrane. Affinity-purified 125I-labeled anti-Rho(D) antibodies bound to intact Rho(D)+ cells, Rho(D)+ membranes, and isolated membrane skeletons from Rho(D)+ cells, but not to Rho(D)- cells. The binding to Rho(D)+ cells was competitively inhibited efficiently by Rho(D)+ membranes and weakly by Rho(D)- membranes. When isolated unsealed Rho(D)+ and Rho(D)- membranes were labeled by lactoperoxidase-catalyzed iodination and solubilized in Triton X-100, Rho(D) polypeptides were immune precipitated only from Rho(D)+ membranes.

Bibliography

Gahmberg, C. G., & Karhi, K. K. (1984). Association of Rho(D) polypeptides with the membrane skeleton in Rho(D)-positive human red cells. The Journal of Immunology, 133(1), 334â337.

Authors 2

C G Gahmberg (first)
K K Karhi (additional)

References 0 Referenced 51

None

Dates

Type	When
Created	2 years, 7 months ago (Dec. 30, 2022, 10:38 p.m.)
Deposited	4 months, 3 weeks ago (March 31, 2025, 10:19 p.m.)
Indexed	1 month, 2 weeks ago (July 4, 2025, 2:32 a.m.)
Issued	41 years, 1 month ago (July 1, 1984)
Published	41 years, 1 month ago (July 1, 1984)
Published Online	41 years, 1 month ago (July 1, 1984)
Published Print	41 years, 1 month ago (July 1, 1984)

Funders 0

None

BibTeX

@article{Gahmberg_1984, title={Association of Rho(D) polypeptides with the membrane skeleton in Rho(D)-positive human red cells.}, volume={133}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.133.1.334}, DOI={10.4049/jimmunol.133.1.334}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Gahmberg, C G and Karhi, K K}, year={1984}, month=jul, pages={334–337} }

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