DOI: 10.4049/jimmunol.133.1.157. Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.

Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.

10.4049/jimmunol.133.1.157

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-1a cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.

Bibliography

Civin, C. I., Strauss, L. C., Brovall, C., Fackler, M. J., Schwartz, J. F., & Shaper, J. H. (1984). Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells. The Journal of Immunology, 133(1), 157â165.

Authors 6

C I Civin (first)
L C Strauss (additional)
C Brovall (additional)
M J Fackler (additional)
J F Schwartz (additional)
J H Shaper (additional)

References 0 Referenced 791

None

Dates

Type	When
Created	2 years, 7 months ago (Dec. 30, 2022, 10:40 p.m.)
Deposited	4 months, 3 weeks ago (March 31, 2025, 10:20 p.m.)
Indexed	4 weeks, 2 days ago (July 26, 2025, 5:12 a.m.)
Issued	41 years, 1 month ago (July 1, 1984)
Published	41 years, 1 month ago (July 1, 1984)
Published Online	41 years, 1 month ago (July 1, 1984)
Published Print	41 years, 1 month ago (July 1, 1984)

Funders 0

None

BibTeX

@article{Civin_1984, title={Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.}, volume={133}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.133.1.157}, DOI={10.4049/jimmunol.133.1.157}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Civin, C I and Strauss, L C and Brovall, C and Fackler, M J and Schwartz, J F and Shaper, J H}, year={1984}, month=jul, pages={157–165} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        26
      ]
    ],
    "date-time": "2025-07-26T09:12:30Z",
    "timestamp": 1753521150321,
    "version": "3.40.3"
  },
  "reference-count": 0,
  "publisher": "Oxford University Press (OUP)",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1984,
            7,
            1
          ]
        ],
        "date-time": "1984-07-01T00:00:00Z",
        "timestamp": 457488000000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "https://academic.oup.com/pages/standard-publication-reuse-rights"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1984,
        7,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>The anti-My-10 mouse monoclonal antibody was raised against the immature human myeloid cell line KG-1a and was selected for nonreactivity with mature human granulocytes. Anti-My-10 immunoprecipitated a KG-1a cell surface protein with an apparent Mr of approximately 115 kD. We describe the binding of this antibody to human hematopoietic cell types and show that My-10 is expressed specifically on immature normal human marrow cells, including hematopoietic progenitor cells. My-10 is also expressed by leukemic marrow cells from a subpopulation of patients. Thus, this antibody allows the identification and purification of hematopoietic progenitor cells from normal human marrow and the subclassification of leukemia.</jats:p>",
  "DOI": "10.4049/jimmunol.133.1.157",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2022,
        12,
        31
      ]
    ],
    "date-time": "2022-12-31T03:40:07Z",
    "timestamp": 1672458007000
  },
  "page": "157-165",
  "source": "Crossref",
  "is-referenced-by-count": 791,
  "title": "Antigenic analysis of hematopoiesis. III. A hematopoietic progenitor cell surface antigen defined by a monoclonal antibody raised against KG-1a cells.",
  "prefix": "10.1093",
  "volume": "133",
  "author": [
    {
      "given": "C I",
      "family": "Civin",
      "sequence": "first",
      "affiliation": [
        {
          "name": "John Hopkins Oncology Center, Divisions of Pediatric Oncology, John Hopkins University School of Medicine , Baltimore, MD"
        }
      ]
    },
    {
      "given": "L C",
      "family": "Strauss",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "John Hopkins Oncology Center, Divisions of Pediatric Oncology, John Hopkins University School of Medicine , Baltimore, MD"
        }
      ]
    },
    {
      "given": "C",
      "family": "Brovall",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "John Hopkins Oncology Center, Divisions of Pediatric Oncology, John Hopkins University School of Medicine , Baltimore, MD"
        }
      ]
    },
    {
      "given": "M J",
      "family": "Fackler",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "John Hopkins Oncology Center, Divisions of Pediatric Oncology, John Hopkins University School of Medicine , Baltimore, MD"
        }
      ]
    },
    {
      "given": "J F",
      "family": "Schwartz",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "John Hopkins Oncology Center, Divisions of Pediatric Oncology, John Hopkins University School of Medicine , Baltimore, MD"
        }
      ]
    },
    {
      "given": "J H",
      "family": "Shaper",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "John Hopkins Oncology Center, Divisions of Pediatric Cell Structure and Function, John Hopkins University School of Medicine , Baltimore, MD"
        },
        {
          "name": "Department of Pharmacology and Experimental Therapeutics, John Hopkins University School of Medicine , Baltimore, MD"
        }
      ]
    }
  ],
  "member": "286",
  "published-online": {
    "date-parts": [
      [
        1984,
        7,
        1
      ]
    ]
  },
  "container-title": "The Journal of Immunology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://academic.oup.com/jimmunol/article-pdf/133/1/157/62446685/157.pdf",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "syndication"
    },
    {
      "URL": "https://academic.oup.com/jimmunol/article-pdf/133/1/157/62446685/157.pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2025,
        4,
        1
      ]
    ],
    "date-time": "2025-04-01T02:20:50Z",
    "timestamp": 1743474050000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://academic.oup.com/jimmunol/article/133/1/157/8085326"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1984,
        7,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1984,
          7,
          1
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.4049/jimmunol.133.1.157",
  "relation": {},
  "ISSN": [
    "0022-1767",
    "1550-6606"
  ],
  "subject": [],
  "published-other": {
    "date-parts": [
      [
        1984,
        7
      ]
    ]
  },
  "published": {
    "date-parts": [
      [
        1984,
        7,
        1
      ]
    ]
  }
}