DOI: 10.4049/jimmunol.131.2.821. Production and characterization of antibodies to thymosin <i>beta</i>4.

Production and characterization of antibodies to thymosin beta4.

10.4049/jimmunol.131.2.821

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Thymosin beta4 antibodies have been raised in rabbits by conjugating the peptide to keyhole limpet hemocyanin, and a radioimmunoassay for thymosin beta4 has been established utilizing tritiated thymosin beta4 as binding ligand. RIA values for thymosin beta4 in rat tissues reveal levels ranging between 8 and 448 micrograms/g wet weight. Highest levels are observed in spleen, although the peptide is also relatively abundant in thymus, lung, and nasal epithelium. Brain, kidney, liver and testis contain only 10 to 20% the concentration of thymosin beta4 seen in spleen, whereas heart and muscle contain only 5 to 10% that of spleen. However, thymosin beta4 probably has a ubiquitous distribution in the rat, being present in all tissues examined. The specificity of the antiserum used in the RIA has been defined. The thymosin beta4 molecule seemingly bears two major epitopes that lie between amino acid residues 1-8 and 22-32. The antiserum does not cross-react with other unrelated peptides. The antiserum will precipitate biosynthetically labeled beta4 from cultured rat peritoneal macrophage.

Bibliography

Goodall, G. J., Hempstead, J. L., & Morgan, J. I. (1983). Production and characterization of antibodies to thymosin beta4. The Journal of Immunology, 131(2), 821â825.

Authors 3

G J Goodall (first)
J L Hempstead (additional)
J I Morgan (additional)

References 0 Referenced 35

None

Dates

Type	When
Created	2 years, 7 months ago (Dec. 30, 2022, 8:52 p.m.)
Deposited	4 months, 3 weeks ago (March 31, 2025, 6:39 p.m.)
Indexed	4 months, 3 weeks ago (March 31, 2025, 7:10 p.m.)
Issued	42 years ago (Aug. 1, 1983)
Published	42 years ago (Aug. 1, 1983)
Published Online	42 years ago (Aug. 1, 1983)
Published Print	42 years ago (Aug. 1, 1983)

Funders 0

None

BibTeX

@article{Goodall_1983, title={Production and characterization of antibodies to thymosin beta4.}, volume={131}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.131.2.821}, DOI={10.4049/jimmunol.131.2.821}, number={2}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Goodall, G J and Hempstead, J L and Morgan, J I}, year={1983}, month=aug, pages={821–825} }

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