DOI: 10.4049/jimmunol.129.6.2548. Immunoelectron microscopic localization of idiotypes and allotypes on immunoglobulin molecules.

Immunoelectron microscopic localization of idiotypes and allotypes on immunoglobulin molecules.

10.4049/jimmunol.129.6.2548

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract We have developed a novel approach to the analysis of antigenic (allotypic and idiotypic) determinants on intact immunoglobulin molecules. Immune complexes composed of IgG in combination with anti-idiotype or anti-allotype antibody were "visualized" by transmission electron microscopy. Individual Fab fragments of anti-idiotype or anti-allotype antibody, when bound to the IgG, altered the "Y" configuration in a reproducible and interpretable manner. Anti-idiotype antibody (either as Fab or IgG) bound to the terminus of the presumed V region of the IgG molecule, thus extending the apparent length of the Fab arms. Analysis of a rabbit VH framework allotype (a1) revealed that the determinant(s) is (are) located on the lateral portion of the V region of IgG. Binding of the anti-a1 Fab fragments was always at approximately right angles to the axis of the Fab arms of IgG. Fab antibody to the rabbit kappa light chain (b4) allotype bound to the lateral portion of the terminal half of the IgG Fab arms. This technique should be of value in localizing less well defined immunoglobulin determinants.

Bibliography

Roux, K. H., & Metzger, D. W. (1982). Immunoelectron microscopic localization of idiotypes and allotypes on immunoglobulin molecules. The Journal of Immunology, 129(6), 2548â2553.

Authors 2

K H Roux (first)
D W Metzger (additional)

References 0 Referenced 36

None

Dates

Type	When
Created	2 years, 7 months ago (Dec. 31, 2022, 6:32 p.m.)
Deposited	4 months, 3 weeks ago (March 31, 2025, 7:29 p.m.)
Indexed	4 months, 3 weeks ago (April 1, 2025, 12:15 a.m.)
Issued	42 years, 8 months ago (Dec. 1, 1982)
Published	42 years, 8 months ago (Dec. 1, 1982)
Published Online	42 years, 8 months ago (Dec. 1, 1982)
Published Print	42 years, 8 months ago (Dec. 1, 1982)

Funders 0

None

BibTeX

@article{Roux_1982, title={Immunoelectron microscopic localization of idiotypes and allotypes on immunoglobulin molecules.}, volume={129}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.129.6.2548}, DOI={10.4049/jimmunol.129.6.2548}, number={6}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Roux, K H and Metzger, D W}, year={1982}, month=dec, pages={2548–2553} }

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