DOI: 10.4049/jimmunol.128.1.129. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants.

Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants.

10.4049/jimmunol.128.1.129

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Thirteen monoclonal antibodies that react with monomorphic determinants on the HLA-A,B,C-beta 2-microglobulin (beta 2m) molecule were characterized. Analysis of antibody activity included inhibition by papain-solubilized HLA antigens and free beta 2m, antibody binding to mouse-human somatic cell hybrids containing human chromosome 6 or 15, and antibody cross-reactivity with lymphocytes from nonhuman species. Two criteria for monomorphism were established: 1) equal inhibition or absorption of antibody activity by all papain-solubilized HLA antigens or cell lines of different HLA specificities tested; and 2) nonpolymorphic cross-reactivity within another species or subspecies. On the basis of soluble antigen inhibition and binding to somatic cell hybrids, 3 classes of antibodies were detected: anti-beta 2m, anti-heavy chain, and anti-complex (against a combinatorial determinant formed by heavy chain and beta 2m). Antibody cross-reaction patterns in nonhuman species were suggestive that these monomorphic antibodies detect a limited number of determinants, minimally one on each chain and 2 combinatorial determinants. Examination of the known primary sequences for HLA-A2, HLA-B7, H-2Kb, and mouse, rabbit and human beta 2m provides a molecular explanation for this limited mouse anti-HLA monomorphic antibody activity.

Authors 2

F M Brodsky (first)
P Parham (additional)

References 0 Referenced 185

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 8:41 p.m.)
Deposited	5 months ago (March 31, 2025, 6:40 p.m.)
Indexed	4 days, 18 hours ago (Sept. 2, 2025, 7 a.m.)
Issued	43 years, 8 months ago (Jan. 1, 1982)
Published	43 years, 8 months ago (Jan. 1, 1982)
Published Online	43 years, 8 months ago (Jan. 1, 1982)
Published Print	43 years, 8 months ago (Jan. 1, 1982)

Funders 0

None

BibTeX

@article{Brodsky_1982, title={Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants.}, volume={128}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.128.1.129}, DOI={10.4049/jimmunol.128.1.129}, number={1}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Brodsky, F M and Parham, P}, year={1982}, month=jan, pages={129–135} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Thirteen monoclonal antibodies that react with monomorphic determinants on the HLA-A,B,C-beta \u00a02-microglobulin (beta \u00a02m) molecule were characterized. Analysis of antibody activity included inhibition by papain-solubilized HLA antigens and free beta \u00a02m, antibody binding to mouse-human somatic cell hybrids containing human chromosome 6 or 15, and antibody cross-reactivity with lymphocytes from nonhuman species. Two criteria for monomorphism were established: 1) equal inhibition or absorption of antibody activity by all papain-solubilized HLA antigens or cell lines of different HLA specificities tested; and 2) nonpolymorphic cross-reactivity within another species or subspecies. On the basis of soluble antigen inhibition and binding to somatic cell hybrids, 3 classes of antibodies were detected: anti-beta \u00a02m, anti-heavy chain, and anti-complex (against a combinatorial determinant formed by heavy chain and beta \u00a02m). Antibody cross-reaction patterns in nonhuman species were suggestive that these monomorphic antibodies detect a limited number of determinants, minimally one on each chain and 2 combinatorial determinants. Examination of the known primary sequences for HLA-A2, HLA-B7, H-2Kb, and mouse, rabbit and human beta \u00a02m provides a molecular explanation for this limited mouse anti-HLA monomorphic antibody activity.</jats:p>",
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