DOI: 10.4049/jimmunol.126.5.1697. <i>In vitro</i> induction of HLA-restricted cytotoxic T lymphocytes against autologous Epstein-Barr Virus transformed B lymphoblastoid cell line.

In vitro induction of HLA-restricted cytotoxic T lymphocytes against autologous Epstein-Barr Virus transformed B lymphoblastoid cell line.

10.4049/jimmunol.126.5.1697

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Cytotoxic T lymphocytes (CTL) against autologous EBV-transformed B lymphoblastoid cell line (LCL) were induced in vitro by culturing peripheral blood lymphocytes (PBL) of healthy donors together with mitomycin C-treated autologous LCL for 6 days. The cytotoxic cells developed only from the E-rosette-positive fraction but not from the negative fraction of PBL. These CTL killed autologous LCL but not PWM-stimulated autologous PBL. In addition, the CTL killed allogeneic LCL when at least 1 of the HLA-A antigens was identical with that of the LCL of CTL donor. However, identity of HLA-B and HLA-C antigens was not enough for a significant killing of allogeneic LCL. The specificity of the CTL was also confirmed by a cold target inhibition test. These results indicated that the CTL induced specifically recognized EBV-transformed cells with HLA restriction.

Bibliography

Fukukawa, T., Hirano, T., Sakaguchi, N., Teranishi, T., Tsuyuguchi, I., Nagao, N., Naito, N., Yoshimura, K., Okubo, Y., Tohda, H., & Oikawa, A. (1981). In vitro induction of HLA-restricted cytotoxic T lymphocytes against autologous Epstein-Barr Virus transformed B lymphoblastoid cell line. The Journal of Immunology, 126(5), 1697â1701.

Authors 11

T Fukukawa (first)
T Hirano (additional)
N Sakaguchi (additional)
T Teranishi (additional)
I Tsuyuguchi (additional)
N Nagao (additional)
N Naito (additional)
K Yoshimura (additional)
Y Okubo (additional)
H Tohda (additional)
A Oikawa (additional)

References 0 Referenced 17

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 7:44 p.m.)
Deposited	5 months ago (March 31, 2025, 5:24 p.m.)
Indexed	5 months ago (April 1, 2025, 12:14 a.m.)
Issued	44 years, 4 months ago (May 1, 1981)
Published	44 years, 4 months ago (May 1, 1981)
Published Online	44 years, 4 months ago (May 1, 1981)
Published Print	44 years, 4 months ago (May 1, 1981)

Funders 0

None

BibTeX

@article{Fukukawa_1981, title={In vitro induction of HLA-restricted cytotoxic T lymphocytes against autologous Epstein-Barr Virus transformed B lymphoblastoid cell line.}, volume={126}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.126.5.1697}, DOI={10.4049/jimmunol.126.5.1697}, number={5}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Fukukawa, T and Hirano, T and Sakaguchi, N and Teranishi, T and Tsuyuguchi, I and Nagao, N and Naito, N and Yoshimura, K and Okubo, Y and Tohda, H and Oikawa, A}, year={1981}, month=may, pages={1697–1701} }

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