DOI: 10.4049/jimmunol.123.4.1548. A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines

A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines

10.4049/jimmunol.123.4.1548

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.

Bibliography

Kearney, J. F., Radbruch, A., Liesegang, B., & Rajewsky, K. (1979). A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines. The Journal of Immunology, 123(4), 1548â1550.

Authors 4

John F Kearney (first)
Andreas Radbruch (additional)
Bernhard Liesegang (additional)
Klaus Rajewsky (additional)

References 0 Referenced 1,262

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 6:51 p.m.)
Deposited	5 months ago (March 31, 2025, 9:26 p.m.)
Indexed	1 day, 14 hours ago (Sept. 4, 2025, 10 a.m.)
Issued	45 years, 11 months ago (Oct. 1, 1979)
Published	45 years, 11 months ago (Oct. 1, 1979)
Published Online	45 years, 11 months ago (Oct. 1, 1979)
Published Print	45 years, 11 months ago (Oct. 1, 1979)

Funders 0

None

BibTeX

@article{Kearney_1979, title={A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell Lines}, volume={123}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.123.4.1548}, DOI={10.4049/jimmunol.123.4.1548}, number={4}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Kearney, John F and Radbruch, Andreas and Liesegang, Bernhard and Rajewsky, Klaus}, year={1979}, month=oct, pages={1548–1550} }

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