DOI: 10.4049/jimmunol.120.2.513. Human Cell Membrane Components Bound to β2-Microglobulin in T Cell-Type Cell Lines

Human Cell Membrane Components Bound to β2-Microglobulin in T Cell-Type Cell Lines

10.4049/jimmunol.120.2.513

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Cell membrane components bound to β2-microglobulin were isolated from Renex 30 (a nonionic detergent)-solubilized membrane materials of two human T cell-type cell lines, MOLT-4 and CCRF-CEM, by gel filtration and lectin affinity chromatography. The isolation was carried out by following the β2-microglobulin activity by radioimmune inhibition assay. The T cell membrane components bound to β2-microglobulin had a uniform molecular size of about 200,000 daltons and most of them showed an affinity to lentil lectin. The isolated membrane components were radioiodinated and examined for identity to HLA antigens by sequential precipitation with rabbit anti-HLA antiserum (specific to HLA large components) and with rabbit anti-β2-microglobulin antiserum. In addition to HLA antigens, the β2-microglobulin-bound components obtained from the MOLT-4 cells were found to contain certain membrane components that are the same in molecular size as the HLA large components but that are different antigenically from the HLA large components. On the other hand, the β2-microglobulin-bound membrane components obtained from the CCRF-CEM cells were all HLA antigens. No other membrane components were involved in the binding.

Bibliography

Tada, N., Tanigaki, N., & Pressman, D. (1978). Human Cell Membrane Components Bound to Î²2-Microglobulin in T Cell-Type Cell Lines. The Journal of Immunology, 120(2), 513â519.

Authors 3

Nobuhiko Tada (first)
Nobuyuki Tanigaki (additional)
David Pressman (additional)

References 0 Referenced 24

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 5:49 p.m.)
Deposited	4 months, 4 weeks ago (March 31, 2025, 5:02 p.m.)
Indexed	4 months, 4 weeks ago (March 31, 2025, 5:40 p.m.)
Issued	47 years, 6 months ago (Feb. 1, 1978)
Published	47 years, 6 months ago (Feb. 1, 1978)
Published Online	47 years, 6 months ago (Feb. 1, 1978)
Published Print	47 years, 6 months ago (Feb. 1, 1978)

Funders 0

None

BibTeX

@article{Tada_1978, title={Human Cell Membrane Components Bound to β2-Microglobulin in T Cell-Type Cell Lines}, volume={120}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.120.2.513}, DOI={10.4049/jimmunol.120.2.513}, number={2}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Tada, Nobuhiko and Tanigaki, Nobuyuki and Pressman, David}, year={1978}, month=feb, pages={513–519} }

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