DOI: 10.4049/jimmunol.116.2.273. Identification of a Macrophage-Specific Cell Surface Antigen

Identification of a Macrophage-Specific Cell Surface Antigen

10.4049/jimmunol.116.2.273

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract A mouse-specific macrophage antigen (MSMA) was identified in NP-40 extracts of 125I-radiolabeled mouse peritoneal macrophages by using a rabbit anti-mouse macrophage serum (AMS) and SDS-polyacrylamide gel electrophoresis. The antigen was shown to have a m.w. of 83,000 daltons and was present on both normal and “activated” peritoneal macrophages. MSMA was also present on syngeneic adherent spleen cells, allogeneic peritoneal macrophages, a mouse macrophage cell line (P388D1), and exhibited some cross-reactivity with peritoneal macrophages from closely related species (rats and hamsters). MSMA was not present on nonadherent peritoneal exudate cells, spleen cells, erythrocytes, thymocytes, or bone marrow cells. Extensive absorptions of AMS with thy - mocytes and erythrocytes from mice were necessary to remove other antibodies that reacted with other mouse membrane antigens. An antiserum directed against a specific membrane antigen has great potential in elucidating structure-function relationships with regard to a number of macrophage activities.

Bibliography

Stinnett, J. D., Kaplan, A. M., & Morahan, P. S. (1976). Identification of a Macrophage-Specific Cell Surface Antigen. The Journal of Immunology, 116(2), 273â278.

Authors 3

J Dwight Stinnett (first)
Alan M Kaplan (additional)
Page S Morahan (additional)

References 0 Referenced 15

None

Dates

Type	When
Created	2 years, 8 months ago (Dec. 30, 2022, 5:17 p.m.)
Deposited	5 months ago (March 31, 2025, 4:47 p.m.)
Indexed	5 months ago (April 1, 2025, 12:13 a.m.)
Issued	49 years, 7 months ago (Feb. 1, 1976)
Published	49 years, 7 months ago (Feb. 1, 1976)
Published Online	49 years, 7 months ago (Feb. 1, 1976)
Published Print	49 years, 7 months ago (Feb. 1, 1976)

Funders 0

None

BibTeX

@article{Stinnett_1976, title={Identification of a Macrophage-Specific Cell Surface Antigen}, volume={116}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.116.2.273}, DOI={10.4049/jimmunol.116.2.273}, number={2}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Stinnett, J Dwight and Kaplan, Alan M and Morahan, Page S}, year={1976}, month=feb, pages={273–278} }

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