DOI: 10.4049/jimmunol.113.6.1752. Antibodies to Proteins Dissolved in Sodium Dodecyl Sulfate

Antibodies to Proteins Dissolved in Sodium Dodecyl Sulfate

10.4049/jimmunol.113.6.1752

Crossref journal-article

Oxford University Press (OUP)

The Journal of Immunology (286)

Abstract

Abstract Rabbits were injected with Drosophila embryo total nonhistone chromosomal (NHC) proteins or with bovine serum albumin, both in 0.1% sodium dodecyl sulfate (SDS). Antibodies were produced which reacted specifically with the protein injected as antigen; the antibodies were not directed against a random denatured protein-SDS complex as shown by failure to cross-react with other proteins in SDS. Antibodies to nonhistone chromosomal proteins were also produced by injecting rabbits with native chromatin. These antibodies reacted with isolated NHC proteins in 0.1% SDS. The presence of low concentrations of SDS did not seem to affect either production of antibodies or their general specificity. The antibodies produced in response to immunization with total NHC proteins or chromatin were directed against at least several proteins as determined by a procedure involving indirect immunofluorescent staining of antibody-antigen complexes in SDS polyacrylamide gels.

Bibliography

Stumph, W. E., Elgin, S. C. R., & Hood, L. (1974). Antibodies to Proteins Dissolved in Sodium Dodecyl Sulfate. The Journal of Immunology, 113(6), 1752â1756.

Authors 3

William E Stumph (first)
Sarah C R Elgin (additional)
Leroy Hood (additional)

References 0 Referenced 49

None

Dates

Type	When
Created	2 years, 7 months ago (Dec. 30, 2022, 4:30 p.m.)
Deposited	4 months, 3 weeks ago (April 4, 2025, 11:03 a.m.)
Indexed	4 months, 3 weeks ago (April 5, 2025, 12:16 a.m.)
Issued	50 years, 8 months ago (Dec. 1, 1974)
Published	50 years, 8 months ago (Dec. 1, 1974)
Published Online	50 years, 8 months ago (Dec. 1, 1974)
Published Print	50 years, 8 months ago (Dec. 1, 1974)

Funders 0

None

BibTeX

@article{Stumph_1974, title={Antibodies to Proteins Dissolved in Sodium Dodecyl Sulfate}, volume={113}, ISSN={1550-6606}, url={http://dx.doi.org/10.4049/jimmunol.113.6.1752}, DOI={10.4049/jimmunol.113.6.1752}, number={6}, journal={The Journal of Immunology}, publisher={Oxford University Press (OUP)}, author={Stumph, William E and Elgin, Sarah C R and Hood, Leroy}, year={1974}, month=dec, pages={1752–1756} }

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