DOI: 10.3928/1081597x-20080601-05. Epithelial Thickness in the Normal Cornea: Three-dimensional Display With Artemis Very High-frequency Digital Ultrasound

Epithelial Thickness in the Normal Cornea: Three-dimensional Display With Artemis Very High-frequency Digital Ultrasound

10.3928/1081597x-20080601-05

Crossref journal-article

SLACK, Inc.

Journal of Refractive Surgery (2308)

Abstract

Purpose To characterize the in vivo epithelial thickness profile in a population of normal eyes. Methods An epithelial thickness profile was measured by Artemis 1 (ArcScan Inc) very high-frequency (VHF) digital ultrasound scanning across the central 10-mm diameter of the cornea of 110 eyes of 56 patients who presented for refractive surgery assessment. The average, standard deviation, minimum, maximum, and range of epithelial thickness were calculated for each point in the 10×10-mm Cartesian matrix and plotted. Differences between the epithelial thickness at the corneal vertex and peripheral locations at the 3-mm radius were calculated. The location of the thinnest epithelium was found for each eye and averaged. Correlations of corneal vertex epithelial thickness with age, spherical equivalent refraction, and average keratometry were calculated. Results The mean epithelial thickness at the corneal vertex was 53.4±4.6 µ m, with no statistically significant difference between right and left eyes, and no significant differences in age, spherical equivalent refraction, or keratometry. The average epithelial thickness map showed that the corneal epithelium was thicker inferiorly than superiorly (5.9 µm at the 3-mm radius, P <.001) and thicker nasally than temporally (1.3 µm at the 3-mm radius, P <.001). The location of the thinnest epithelium was displaced on average 0.33 mm temporally and 0.90 mm superiorly with reference to the corneal vertex. Conclusions Three-dimensional thickness mapping of the corneal epithelium demonstrated that the epithelial thickness is not evenly distributed across the cornea; the epithelium was significantly thicker inferiorly than superiorly and significantly thicker nasally than temporally with a larger inferosuperior difference than nasotemporal difference. [ J Refract Surg. 2008;24:571–581.]

Bibliography

Reinstein, D. Z., Archer, T. J., Gobbe, M., Silverman, R. H., & Coleman, D. J. (2008). Epithelial Thickness in the Normal Cornea: Three-dimensional Display With Artemis Very High-frequency Digital Ultrasound. Journal of Refractive Surgery, 24(6), 571â581.

Authors 5

Dan Z. Reinstein (first)
Timothy J. Archer (additional)
Marine Gobbe (additional)
Ronald H. Silverman (additional)
D. Jackson Coleman (additional)

References 35 Referenced 293

Hogan MJ, Alvarado JA, Weddell JE. Histology of the Human Eye. Philadelphia, Pa: Saunders Publishing; 1971. / Histology of the Human Eye by Hogan MJ (1971)
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Patel SV, McLaren JW, Hodge DO, Bourne WM. Confocal microscopy in vivo in corneas of long-term contact lens wearers. Invest Ophthalmol Vis Sci. 2002;43:995–1003. / Invest Ophthalmol Vis Sci / Confocal microscopy in vivo in corneas of long-term contact lens wearers by Patel SV (2002)
10.1016/S0161-6420(94)31314-5
Reinstein DZ, Silverman RH, Trokel SL, Rondeau MJ, Coleman DJ. High-frequency ultrasound three dimensional pachymetry and keratometry of the cornea. Presented at: Association for Research in Vision and Ophthalmology; May 2–7, 1993; Sarasota, Fla. / Presented at: Association for Research in Vision and Ophthalmology by Reinstein DZ
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Møller-Pedersen T, Li HF, Petroll WM, Cavanagh HD, Jester JV. Confocal microscopic characterization of wound repair after photorefractive keratectomy. Invest Ophthalmol Vis Sci. 1998;39:487–501. / Invest Ophthalmol Vis Sci / Confocal microscopic characterization of wound repair after photorefractive keratectomy by Møller-Pedersen T (1998)
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10.3928/1081-597X-20000301-05
10.3928/1081-597X-20050901-10
10.1016/S0161-6420(94)38032-8

Dates

Type	When
Created	6 years, 4 months ago (April 11, 2019, 11:47 a.m.)
Deposited	10 months, 2 weeks ago (Oct. 14, 2024, 12:50 p.m.)
Indexed	4 hours, 7 minutes ago (Sept. 3, 2025, 5:53 a.m.)
Issued	17 years, 3 months ago (June 1, 2008)
Published	17 years, 3 months ago (June 1, 2008)
Published Print	17 years, 3 months ago (June 1, 2008)

Funders 0

None

BibTeX

@article{Reinstein_2008, title={Epithelial Thickness in the Normal Cornea: Three-dimensional Display With Artemis Very High-frequency Digital Ultrasound}, volume={24}, ISSN={1081-597X}, url={http://dx.doi.org/10.3928/1081597x-20080601-05}, DOI={10.3928/1081597x-20080601-05}, number={6}, journal={Journal of Refractive Surgery}, publisher={SLACK, Inc.}, author={Reinstein, Dan Z. and Archer, Timothy J. and Gobbe, Marine and Silverman, Ronald H. and Coleman, D. Jackson}, year={2008}, month=jun, pages={571–581} }

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