DOI: 10.2337/diab.16.1.35. Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas

Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas

10.2337/diab.16.1.35

Crossref journal-article

American Diabetes Association

Diabetes (1167)

Abstract

A simple method for the isolation of intact islets from the normal rat pancreas is described. The method is based upon disruption of the acinar parenchyma by injecting Hanks solution into the pancreatic duct system followed by incubation of the pancreas in collagenase. Islets can be separated rapidly from this mixture by sedimentation. The isolated islets release insulin in vitro and appear normal by light and electron microscopy after incubation. A centrifugation method is also described for isolation of large quantities of islets for biochemical studies.

Bibliography

Lacy, P. E., & Kostianovsky, M. (1967). Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas. Diabetes, 16(1), 35â39.

Authors 2

Paul E Lacy (first)
Mery Kostianovsky (additional)

References 0 Referenced 2,016

None

Dates

Type	When
Created	10 years, 8 months ago (Dec. 11, 2014, 12:37 a.m.)
Deposited	2 years, 9 months ago (Nov. 2, 2022, 12:12 p.m.)
Indexed	1 day, 11 hours ago (Aug. 26, 2025, 3:15 a.m.)
Issued	58 years, 7 months ago (Jan. 1, 1967)
Published	58 years, 7 months ago (Jan. 1, 1967)
Published Print	58 years, 7 months ago (Jan. 1, 1967)

Funders 0

None

BibTeX

@article{Lacy_1967, title={Method for the Isolation of Intact Islets of Langerhans from the Rat Pancreas}, volume={16}, ISSN={1939-327X}, url={http://dx.doi.org/10.2337/diab.16.1.35}, DOI={10.2337/diab.16.1.35}, number={1}, journal={Diabetes}, publisher={American Diabetes Association}, author={Lacy, Paul E and Kostianovsky, Mery}, year={1967}, month=jan, pages={35–39} }

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