DOI: 10.1002/j.1537-2197.1965.tb06809.x. ELECTRON MICROSCOPY OF CARTERIA AND CHLAMYDOMONAS

ELECTRON MICROSCOPY OF CARTERIA AND CHLAMYDOMONAS

10.1002/j.1537-2197.1965.tb06809.x

Crossref journal-article

Wiley

American Journal of Botany (311)

Abstract

Cultures of Chlamydomonas eugametos, Chl. sp., Carteria eugametos, C. crucifera, C. radiosa, and C. sp. were examined with the electron microscope to determine generic differences between Carteria and Chlamydomonas at the ultrastructural level. The ultrastructure of the flagella, mitochondria, dictyosomes, nuclei and ground substance was noted to be similar in all species. The cellular boundary of all species except Chlamydomonas eugametos contains a 250 A intermediate layer of unknown chemical composition between the fibrillar cellulose wall and the outer capsule layer. Four structural features other than the number of flagella distinguish Carteria from Chlamydomonas: the intermediate layer of the cellular boundary, the chloroplast, the pyrenoid and the eyespot. Only in the Carteria species is the intermediate layer traversed by striations or 12‐mμ‐wide bars. Striations in the cellulose wall surrounding the flagellar channels also appear in Carteria eugametos and C. crucifera. The chloroplast lamellae of the Carteria species are grouped into discrete stacks of invaginated thylakoids termed pseudograna. The chloroplast lamellae of Chlamydomonas are broad and sheet‐like and are also invaginated although less frequently than are the pseudograna of Carteria. The phenomenon of infolding of the chloroplast lamellae is suggested as a general developmental process in the formation of new thylakoids. In Carteria, single thylakoids traverse the pyrenoid and there are 2 rows of granules in the eyespot. Favorable micrographs of the eyespot indicate that the granules may be osmiophilic granules of the chloroplast chemically modified for a photoreceptive function.

Bibliography

Lembi, C. A., & Lang, N. J. (1965). ELECTRON MICROSCOPY OF CARTERIA AND CHLAMYDOMONAS. American Journal of Botany, 52(5), 464â477. Portico.

Authors 2

Carole A. Lembi (first)
Norma J. Lang (additional)

References 32 Referenced 55

10.1111/j.1550-7408.1955.tb02404.x
{'key': 'e_1_2_1_3_1', 'volume-title': 'Plant biochemistry', 'author': 'Bonner J.', 'year': '1950'} / Plant biochemistry by Bonner J. (1950)
{'key': 'e_1_2_1_4_1', 'first-page': '97', 'article-title': 'Morphologie und Biologie des Algenstärkekornes', 'volume': '45', 'author': 'Czurda V.', 'year': '1928', 'journal-title': 'Beih. Bot. Centralbl.'} / Beih. Bot. Centralbl. / Morphologie und Biologie des Algenstärkekornes by Czurda V. (1928)
10.1007/BF01928209
10.1016/B978-0-12-123302-0.50011-3
10.1083/jcb.13.3.437
{'key': 'e_1_2_1_8_1', 'volume-title': 'The structure and reproduction of the algae', 'author': 'Fritsch F. E.', 'year': '1961'} / The structure and reproduction of the algae by Fritsch F. E. (1961)
10.1083/jcb.7.4.697
10.1016/S0022-5320(62)90022-9
10.1016/S0022-5320(62)90038-2
10.1016/0014-4827(58)90112-5
10.2216/i0031-8884-3-1-37.1
10.1111/j.1469-8137.1953.tb07517.x
10.1093/oxfordjournals.aob.a083588
10.1002/j.1537-2197.1963.tb12235.x
10.1111/j.1550-7408.1963.tb01685.x
10.1083/jcb.19.3.631
10.1093/jxb/13.2.325
10.1111/j.1365-2818.1964.tb04483.x
{'key': 'e_1_2_1_21_1', 'first-page': '197', 'article-title': 'Structure and function of the eyespot in unicellular and colonial organisms', 'volume': '60', 'author': 'Mast S. O.', 'year': '1927', 'journal-title': 'Arch. Protistenk.'} / Arch. Protistenk. / Structure and function of the eyespot in unicellular and colonial organisms by Mast S. O. (1927)
10.1146/annurev.pp.13.060162.000331
10.1016/B978-0-12-123302-0.50012-5
1927 A. Pascher Die Süsswasserflora Deutschlands Österreichs und der Schweiz
{'key': 'e_1_2_1_25_1', 'first-page': '224', 'article-title': 'Zur Verwendbarkeit von Rutheniumrot als elektronenmikroskopisches Kontrastierungsmittel', 'volume': '16', 'author': 'Reimann B.', 'year': '1961', 'journal-title': 'Mikroscopie'} / Mikroscopie / Zur Verwendbarkeit von Rutheniumrot als elektronenmikroskopisches Kontrastierungsmittel by Reimann B. (1961)
{'key': 'e_1_2_1_26_1', 'first-page': '1', 'volume-title': 'The structure and function of subcellular components, Biochem', 'author': 'Robertson J. D.', 'year': '1959'} / The structure and function of subcellular components, Biochem by Robertson J. D. (1959)
10.1083/jcb.3.3.463
10.1111/j.1550-7408.1960.tb00712.x
10.1007/BF01247841
{'key': 'e_1_2_1_30_1', 'volume-title': 'Freshwater algae of the United States', 'author': 'Smith G. M.', 'year': '1950'} / Freshwater algae of the United States by Smith G. M. (1950)
10.2307/2439496
10.1016/S0022-5320(63)80024-6
10.1083/jcb.18.2.419

Dates

Type	When
Created	7 years, 6 months ago (Jan. 31, 2018, 1:16 a.m.)
Deposited	1 year, 11 months ago (Sept. 26, 2023, 1:36 a.m.)
Indexed	21 hours, 14 minutes ago (Aug. 29, 2025, 6:22 a.m.)
Issued	60 years, 3 months ago (May 1, 1965)
Published	60 years, 3 months ago (May 1, 1965)
Published Online	60 years, 3 months ago (May 1, 1965)
Published Print	60 years, 3 months ago (May 1, 1965)

Funders 1

National Science Foundation 10.13039/100000001
Region: Americas
gov (National government)
Labels4
1. U.S. National Science Foundation
2. NSF
3. US NSF
4. USA NSF
Awards1
1. G21796

BibTeX

@article{Lembi_1965, title={ELECTRON MICROSCOPY OF CARTERIA AND CHLAMYDOMONAS}, volume={52}, ISSN={1537-2197}, url={http://dx.doi.org/10.1002/j.1537-2197.1965.tb06809.x}, DOI={10.1002/j.1537-2197.1965.tb06809.x}, number={5}, journal={American Journal of Botany}, publisher={Wiley}, author={Lembi, Carole A. and Lang, Norma J.}, year={1965}, month=may, pages={464–477} }

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