DOI: 10.1523/jneurosci.4749-05.2006. Coordination and Modulation of Locomotion Pattern Generators in<i>Drosophila</i>Larvae: Effects of Altered Biogenic Amine Levels by the Tyramine β Hydroxlyase Mutation

Coordination and Modulation of Locomotion Pattern Generators inDrosophilaLarvae: Effects of Altered Biogenic Amine Levels by the Tyramine β Hydroxlyase Mutation

10.1523/jneurosci.4749-05.2006

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

Forward locomotion ofDrosophila melanogasterlarvae is composed of rhythmic waves of contractions that are thought to be produced by segmentally organized central pattern generators. We present a systematic description of spike activity patterns during locomotive contraction waves in semi-intact wild-type and mutant larval preparations. We have shown previously thatTβhnM18mutants, with altered levels of octopamine and tyramine, have a locomotion deficit. By recordingen passantfrom the segmental nerves, we investigated the coordination of the neuronal activity driving contraction waves of the abdominal body-wall muscles. Rhythmic bursts of activity that occurred concurrently with locomotive waves were frequently observed in wild-type larvae but were rarely seen inTβhnM18mutants. These centrally generated patterned activities were eliminated in the distal stumps of both wild-type andTβhnM18larvae after severing the segmental nerve from the CNS. Patterned activities persisted in the proximal stumps deprived of sensory feedback from the periphery. Simultaneous recordings demonstrated a delay in the bursting activity between different segments, with greater delay for segments that were farther apart. In contrast, bilateral recordings within a single segment revealed a well synchronized activity pattern in nerves innervating each hemisegment in both wild-type andTβhnM18larvae. Significantly, rhythmic patterns of bursts and waves could be evoked inTβhnM18mutants by head or tail stimulation despite their highly irregular spontaneous activities. These observations suggest a role of the biogenic amines in the initiation and modulation of motor pattern generation. The technique presented here can be readily extended to examine the locomotion motor program of other mutants.

Bibliography

Fox, L. E., Soll, D. R., & Wu, C.-F. (2006). Coordination and Modulation of Locomotion Pattern Generators inDrosophilaLarvae: Effects of Altered Biogenic Amine Levels by the Tyramine Î² Hydroxlyase Mutation. The Journal of Neuroscience, 26(5), 1486â1498.

Authors 3

Lyle E. Fox (first)
David R. Soll (additional)
Chun-Fang Wu (additional)

References 61 Referenced 149

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10.1242/jeb.121.1.115 / J Exp Biol ((1986))
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Fox LE, Ueda A, Berke B, Peng IF, Wu CF (2004) Movement disorders in Drosophila mutants of potassium channels and biogenic amine pathways. In: Animal models of movement disorders (LeDoux MS, ed.) , pp. 487–504. San Diego: Academic/Elsevier. (10.1016/B978-012088382-0/50045-1)
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{'key': '2023041303163904000_26.5.1486.61', 'first-page': '4047', 'volume': '8', 'year': '(1978)', 'journal-title': 'Proc Natl Acad Sci USA'} / Proc Natl Acad Sci USA ((1978))

Dates

Type	When
Created	19 years, 6 months ago (Feb. 1, 2006, 7:13 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 1:13 p.m.)
Indexed	30 minutes ago (Aug. 26, 2025, 2:23 a.m.)
Issued	19 years, 6 months ago (Feb. 1, 2006)
Published	19 years, 6 months ago (Feb. 1, 2006)
Published Online	19 years, 6 months ago (Feb. 1, 2006)
Published Print	19 years, 6 months ago (Feb. 1, 2006)

Funders 0

None

BibTeX

@article{Fox_2006, title={Coordination and Modulation of Locomotion Pattern Generators inDrosophilaLarvae: Effects of Altered Biogenic Amine Levels by the Tyramine β Hydroxlyase Mutation}, volume={26}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.4749-05.2006}, DOI={10.1523/jneurosci.4749-05.2006}, number={5}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Fox, Lyle E. and Soll, David R. and Wu, Chun-Fang}, year={2006}, month=feb, pages={1486–1498} }

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