DOI: 10.1523/jneurosci.4703-03.2004. Direct Impact of T Cells on Neurons Revealed by Two-Photon Microscopy in Living Brain Tissue

Direct Impact of T Cells on Neurons Revealed by Two-Photon Microscopy in Living Brain Tissue

10.1523/jneurosci.4703-03.2004

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

Encephalitogenic T cells invade the brain during neuroinflammation such as multiple sclerosis (MS), inducing damage to myelin sheaths and oligodendrocytes. Only recently, neuronal structures were reported to be a crucial target in the disease. Here, two-photon microscopy using ion-sensitive dyes revealed that within the complex cellular network of living brain tissue, proteolipid protein (PLP)-specific T cells and T cells recognizing the nonmurine antigen ovalbumin (OVA) directly and independently of the major histocompatibility complex (MHC) contact neurons in which they induce calcium oscillations. T cell contact finally resulted in a lethal increase in neuronal calcium levels. This could be prevented by blocking both perforin and glutamate receptors. For the first time, our data provide direct insight into the activity of T cells in the living brain and their detrimental impact on neurons.

Bibliography

Nitsch, R., Pohl, E. E., Smorodchenko, A., Infante-Duarte, C., Aktas, O., & Zipp, F. (2004). Direct Impact of T Cells on Neurons Revealed by Two-Photon Microscopy in Living Brain Tissue. The Journal of Neuroscience, 24(10), 2458â2464.

Authors 6

Robert Nitsch (first)
Elena E. Pohl (additional)
Alina Smorodchenko (additional)
Carmen Infante-Duarte (additional)
Orhan Aktas (additional)
Frauke Zipp (additional)

References 35 Referenced 119

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Dates

Type	When
Created	21 years, 5 months ago (March 10, 2004, 5:52 p.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 12:32 p.m.)
Indexed	2 days, 22 hours ago (Sept. 3, 2025, 5:51 a.m.)
Issued	21 years, 5 months ago (March 10, 2004)
Published	21 years, 5 months ago (March 10, 2004)
Published Online	21 years, 5 months ago (March 10, 2004)
Published Print	21 years, 5 months ago (March 10, 2004)

Funders 0

None

BibTeX

@article{Nitsch_2004, title={Direct Impact of T Cells on Neurons Revealed by Two-Photon Microscopy in Living Brain Tissue}, volume={24}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.4703-03.2004}, DOI={10.1523/jneurosci.4703-03.2004}, number={10}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Nitsch, Robert and Pohl, Elena E. and Smorodchenko, Alina and Infante-Duarte, Carmen and Aktas, Orhan and Zipp, Frauke}, year={2004}, month=mar, pages={2458–2464} }

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