DOI: 10.1523/jneurosci.3727-03.2004. Stereotyped Odor-Evoked Activity in the Mushroom Body of<i>Drosophila</i>Revealed by Green Fluorescent Protein-Based Ca<sup>2+</sup>Imaging

Stereotyped Odor-Evoked Activity in the Mushroom Body ofDrosophilaRevealed by Green Fluorescent Protein-Based Ca2+Imaging

10.1523/jneurosci.3727-03.2004

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

To study the representation of olfactory information in higher brain centers, we expressed a green fluorescent protein-based Ca2+sensor, G-CaMP, in theDrosophilamushroom body (MB). Using two-photon microscopy, we imaged odor-evoked G-CaMP fluorescence transients in MB neurons [Kenyon cells (KCs)] with single-cell resolution. Odors produced large fluorescence transients in a subset of KC somata and in restricted regions of the calyx, the neuropil of the MB. In different KCs, odor-evoked fluorescence transients showed diverse changes with odor concentration: in some KCs, fluorescence transients were evoked by an odor at concentrations spanning several orders of magnitude, whereas in others only at a narrow concentration range. Different odors produced fluorescence transients in different subsets of KCs. The spatial distributions of KCs showing fluorescence transients evoked by a given odor were similar across individuals. For some odors, individual KCs with fluorescence transients evoked by a particular odor could be found in similar locations in different flies with spatial precisions on the order of the size of KC somata. These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB.

Bibliography

Wang, Y., Guo, H.-F., Pologruto, T. A., Hannan, F., Hakker, I., Svoboda, K., & Zhong, Y. (2004). Stereotyped Odor-Evoked Activity in the Mushroom Body ofDrosophilaRevealed by Green Fluorescent Protein-Based Ca2+Imaging. The Journal of Neuroscience, 24(29), 6507â6514.

Authors 7

Yalin Wang (first)
Hui-Fu Guo (additional)
Thomas A. Pologruto (additional)
Frances Hannan (additional)
Inessa Hakker (additional)
Karel Svoboda (additional)
Yi Zhong (additional)

References 50 Referenced 170

10.1152/jn.1994.71.2.808 / J Neurophysiol (1994)
10.1016/S0960-9822(01)00409-2
10.1242/dev.118.2.401 / Development (1993)
10.1016/S0092-8674(00)80698-4
10.1016/S0092-8674(94)90562-2
10.1016/S0896-6273(00)81093-4
10.1126/science.274.5295.2104
10.1016/S0896-6273(01)00289-6
10.1016/S0896-6273(00)81237-4
10.1038/8144
10.1038/77680
10.1111/j.1469-7793.1999.759ad.x
10.1523/JNEUROSCI.07-05-01361.1987 / J Neurosci (1987)
10.1146/annurev.neuro.20.1.595
10.1038/35102574
10.1002/(SICI)1096-9861(19980420)393:4<457::AID-CNE5>3.0.CO;2-#
10.1146/annurev.neuro.24.1.963
10.1016/S0959-4388(02)00348-3
10.1038/35009107
10.1016/S0092-8674(00)80581-4
10.1016/S0006-3495(00)76809-3
10.1016/S0092-8674(02)00700-6
10.1016/S0092-8674(00)81387-2
10.1038/84397
10.1152/jn.1983.49.1.188 / J Neurophysiol (1983)
10.1152/jn.1999.82.2.1091 / J Neurophysiol (1999)
10.1126/science.1070502
10.1186/1475-925X-2-13
10.1016/S0960-9822(02)00656-5
10.1016/0092-8674(94)90015-9
10.1016/0006-8993(88)90170-9
10.1016/S0896-6273(00)80803-X
10.1152/jn.1995.74.2.733 / J Neurophysiol (1995)
Skoulakis E, Acevedo S (2003) Characterization of a novel non-associative behavior, “protection from habituation,” defined by D14-3-3zeta mutants.
10.1126/science.6289435
10.1007/BF00213783
10.1016/j.neuron.2003.08.011
10.1523/JNEUROSCI.23-27-09246.2003 / J Neurosci (2003)
10.1038/385161a0
10.1038/295405a0
10.1016/S0092-8674(00)80582-6
10.1016/S0092-8674(00)00021-0
10.1016/S0896-6273(01)00506-2
10.1016/S0092-8674(03)00004-7
10.1016/S0896-6273(01)00196-9
10.1152/jn.1997.77.1.186 / J Neurophysiol (1997)
10.1016/S0092-8674(02)00707-9
10.1002/cne.10155
10.1523/JNEUROSCI.23-01-00064.2003 / J Neurosci (2003)
10.1038/35102506

Dates

Type	When
Created	21 years, 1 month ago (July 21, 2004, 6:11 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 12:14 p.m.)
Indexed	1 month, 1 week ago (July 19, 2025, 11:18 p.m.)
Issued	21 years, 1 month ago (July 21, 2004)
Published	21 years, 1 month ago (July 21, 2004)
Published Online	21 years, 1 month ago (July 21, 2004)
Published Print	21 years, 1 month ago (July 21, 2004)

Funders 0

None

BibTeX

@article{Wang_2004, title={Stereotyped Odor-Evoked Activity in the Mushroom Body ofDrosophilaRevealed by Green Fluorescent Protein-Based Ca2+Imaging}, volume={24}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.3727-03.2004}, DOI={10.1523/jneurosci.3727-03.2004}, number={29}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Wang, Yalin and Guo, Hui-Fu and Pologruto, Thomas A. and Hannan, Frances and Hakker, Inessa and Svoboda, Karel and Zhong, Yi}, year={2004}, month=jul, pages={6507–6514} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        20
      ]
    ],
    "date-time": "2025-07-20T03:18:46Z",
    "timestamp": 1752981526503
  },
  "reference-count": 50,
  "publisher": "Society for Neuroscience",
  "issue": "29",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            1,
            21
          ]
        ],
        "date-time": "2005-01-21T00:00:00Z",
        "timestamp": 1106265600000
      },
      "content-version": "vor",
      "delay-in-days": 184,
      "URL": "https://creativecommons.org/licenses/by-nc-sa/4.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2004,
        7,
        21
      ]
    ]
  },
  "abstract": "<jats:p>To study the representation of olfactory information in higher brain centers, we expressed a green fluorescent protein-based Ca<jats:sup>2+</jats:sup>sensor, G-CaMP, in the<jats:italic>Drosophila</jats:italic>mushroom body (MB). Using two-photon microscopy, we imaged odor-evoked G-CaMP fluorescence transients in MB neurons [Kenyon cells (KCs)] with single-cell resolution. Odors produced large fluorescence transients in a subset of KC somata and in restricted regions of the calyx, the neuropil of the MB. In different KCs, odor-evoked fluorescence transients showed diverse changes with odor concentration: in some KCs, fluorescence transients were evoked by an odor at concentrations spanning several orders of magnitude, whereas in others only at a narrow concentration range. Different odors produced fluorescence transients in different subsets of KCs. The spatial distributions of KCs showing fluorescence transients evoked by a given odor were similar across individuals. For some odors, individual KCs with fluorescence transients evoked by a particular odor could be found in similar locations in different flies with spatial precisions on the order of the size of KC somata. These results indicate that odor-evoked activity can have remarkable spatial specificity in the MB.</jats:p>",
  "DOI": "10.1523/jneurosci.3727-03.2004",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2004,
        7,
        21
      ]
    ],
    "date-time": "2004-07-21T22:11:36Z",
    "timestamp": 1090447896000
  },
  "page": "6507-6514",
  "source": "Crossref",
  "is-referenced-by-count": 170,
  "title": "Stereotyped Odor-Evoked Activity in the Mushroom Body of<i>Drosophila</i>Revealed by Green Fluorescent Protein-Based Ca<sup>2+</sup>Imaging",
  "prefix": "10.1523",
  "volume": "24",
  "author": [
    {
      "given": "Yalin",
      "family": "Wang",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Hui-Fu",
      "family": "Guo",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Thomas A.",
      "family": "Pologruto",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Frances",
      "family": "Hannan",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Inessa",
      "family": "Hakker",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Karel",
      "family": "Svoboda",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Yi",
      "family": "Zhong",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "393",
  "published-online": {
    "date-parts": [
      [
        2004,
        7,
        21
      ]
    ]
  },
  "reference": [
    {
      "key": "2023041303074086000_24.29.6507.1",
      "doi-asserted-by": "crossref",
      "first-page": "808",
      "DOI": "10.1152/jn.1994.71.2.808",
      "volume": "71",
      "year": "1994",
      "journal-title": "J Neurophysiol"
    },
    {
      "key": "2023041303074086000_24.29.6507.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(01)00409-2"
    },
    {
      "key": "2023041303074086000_24.29.6507.3",
      "doi-asserted-by": "crossref",
      "first-page": "401",
      "DOI": "10.1242/dev.118.2.401",
      "volume": "118",
      "year": "1993",
      "journal-title": "Development"
    },
    {
      "key": "2023041303074086000_24.29.6507.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80698-4"
    },
    {
      "key": "2023041303074086000_24.29.6507.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(94)90562-2"
    },
    {
      "key": "2023041303074086000_24.29.6507.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)81093-4"
    },
    {
      "key": "2023041303074086000_24.29.6507.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.274.5295.2104"
    },
    {
      "key": "2023041303074086000_24.29.6507.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(01)00289-6"
    },
    {
      "key": "2023041303074086000_24.29.6507.9",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)81237-4"
    },
    {
      "key": "2023041303074086000_24.29.6507.10",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/8144"
    },
    {
      "key": "2023041303074086000_24.29.6507.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/77680"
    },
    {
      "key": "2023041303074086000_24.29.6507.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1469-7793.1999.759ad.x"
    },
    {
      "key": "2023041303074086000_24.29.6507.13",
      "doi-asserted-by": "crossref",
      "first-page": "1361",
      "DOI": "10.1523/JNEUROSCI.07-05-01361.1987",
      "volume": "7",
      "year": "1987",
      "journal-title": "J Neurosci"
    },
    {
      "key": "2023041303074086000_24.29.6507.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.neuro.20.1.595"
    },
    {
      "key": "2023041303074086000_24.29.6507.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35102574"
    },
    {
      "key": "2023041303074086000_24.29.6507.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1096-9861(19980420)393:4<457::AID-CNE5>3.0.CO;2-#"
    },
    {
      "key": "2023041303074086000_24.29.6507.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.neuro.24.1.963"
    },
    {
      "key": "2023041303074086000_24.29.6507.18",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-4388(02)00348-3"
    },
    {
      "key": "2023041303074086000_24.29.6507.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35009107"
    },
    {
      "key": "2023041303074086000_24.29.6507.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80581-4"
    },
    {
      "key": "2023041303074086000_24.29.6507.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(00)76809-3"
    },
    {
      "key": "2023041303074086000_24.29.6507.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)00700-6"
    },
    {
      "key": "2023041303074086000_24.29.6507.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81387-2"
    },
    {
      "key": "2023041303074086000_24.29.6507.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/84397"
    },
    {
      "key": "2023041303074086000_24.29.6507.25",
      "doi-asserted-by": "crossref",
      "first-page": "188",
      "DOI": "10.1152/jn.1983.49.1.188",
      "volume": "49",
      "year": "1983",
      "journal-title": "J Neurophysiol"
    },
    {
      "key": "2023041303074086000_24.29.6507.26",
      "doi-asserted-by": "crossref",
      "first-page": "1091",
      "DOI": "10.1152/jn.1999.82.2.1091",
      "volume": "82",
      "year": "1999",
      "journal-title": "J Neurophysiol"
    },
    {
      "key": "2023041303074086000_24.29.6507.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1070502"
    },
    {
      "key": "2023041303074086000_24.29.6507.28",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1475-925X-2-13"
    },
    {
      "key": "2023041303074086000_24.29.6507.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(02)00656-5"
    },
    {
      "key": "2023041303074086000_24.29.6507.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(94)90015-9"
    },
    {
      "key": "2023041303074086000_24.29.6507.31",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(88)90170-9"
    },
    {
      "key": "2023041303074086000_24.29.6507.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80803-X"
    },
    {
      "key": "2023041303074086000_24.29.6507.33",
      "doi-asserted-by": "crossref",
      "first-page": "733",
      "DOI": "10.1152/jn.1995.74.2.733",
      "volume": "74",
      "year": "1995",
      "journal-title": "J Neurophysiol"
    },
    {
      "key": "2023041303074086000_24.29.6507.34",
      "unstructured": "Skoulakis E, Acevedo S (2003) Characterization of a novel non-associative behavior, \u201cprotection from habituation,\u201d defined by D14-3-3zeta mutants."
    },
    {
      "key": "2023041303074086000_24.29.6507.35",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.6289435"
    },
    {
      "key": "2023041303074086000_24.29.6507.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00213783"
    },
    {
      "key": "2023041303074086000_24.29.6507.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.neuron.2003.08.011"
    },
    {
      "key": "2023041303074086000_24.29.6507.38",
      "doi-asserted-by": "crossref",
      "first-page": "9246",
      "DOI": "10.1523/JNEUROSCI.23-27-09246.2003",
      "volume": "23",
      "year": "2003",
      "journal-title": "J Neurosci"
    },
    {
      "key": "2023041303074086000_24.29.6507.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/385161a0"
    },
    {
      "key": "2023041303074086000_24.29.6507.40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/295405a0"
    },
    {
      "key": "2023041303074086000_24.29.6507.41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80582-6"
    },
    {
      "key": "2023041303074086000_24.29.6507.42",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)00021-0"
    },
    {
      "key": "2023041303074086000_24.29.6507.43",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(01)00506-2"
    },
    {
      "key": "2023041303074086000_24.29.6507.44",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(03)00004-7"
    },
    {
      "key": "2023041303074086000_24.29.6507.45",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(01)00196-9"
    },
    {
      "key": "2023041303074086000_24.29.6507.46",
      "doi-asserted-by": "crossref",
      "first-page": "186",
      "DOI": "10.1152/jn.1997.77.1.186",
      "volume": "77",
      "year": "1997",
      "journal-title": "J Neurophysiol"
    },
    {
      "key": "2023041303074086000_24.29.6507.47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(02)00707-9"
    },
    {
      "key": "2023041303074086000_24.29.6507.48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.10155"
    },
    {
      "key": "2023041303074086000_24.29.6507.49",
      "doi-asserted-by": "crossref",
      "first-page": "64",
      "DOI": "10.1523/JNEUROSCI.23-01-00064.2003",
      "volume": "23",
      "year": "2003",
      "journal-title": "J Neurosci"
    },
    {
      "key": "2023041303074086000_24.29.6507.50",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35102506"
    }
  ],
  "container-title": "The Journal of Neuroscience",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1523/JNEUROSCI.3727-03.2004",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        4,
        13
      ]
    ],
    "date-time": "2023-04-13T16:14:28Z",
    "timestamp": 1681402468000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.3727-03.2004"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2004,
        7,
        21
      ]
    ]
  },
  "references-count": 50,
  "journal-issue": {
    "issue": "29",
    "published-online": {
      "date-parts": [
        [
          2004,
          7,
          21
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          2004,
          7,
          21
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1523/JNEUROSCI.3727-03.2004"
  ],
  "URL": "http://dx.doi.org/10.1523/JNEUROSCI.3727-03.2004",
  "relation": {
    "has-review": [
      {
        "id-type": "doi",
        "id": "10.3410/f.1020264.236426",
        "asserted-by": "object"
      },
      {
        "id-type": "doi",
        "id": "10.3410/f.1020264.232471",
        "asserted-by": "object"
      }
    ]
  },
  "ISSN": [
    "0270-6474",
    "1529-2401"
  ],
  "subject": [],
  "container-title-short": "J. Neurosci.",
  "published": {
    "date-parts": [
      [
        2004,
        7,
        21
      ]
    ]
  }
}