DOI: 10.1523/jneurosci.23-26-08881.2003. Upregulation of Sodium Channel Na<sub>v</sub>1.3 and Functional Involvement in Neuronal Hyperexcitability Associated with Central Neuropathic Pain after Spinal Cord Injury

Upregulation of Sodium Channel Nav1.3 and Functional Involvement in Neuronal Hyperexcitability Associated with Central Neuropathic Pain after Spinal Cord Injury

10.1523/jneurosci.23-26-08881.2003

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

Spinal cord injury (SCI) can result in hyperexcitability of dorsal horn neurons and central neuropathic pain. We hypothesized that these phenomena are consequences, in part, of dysregulated expression of voltage-gated sodium channels. Because the rapidly repriming TTX-sensitive sodium channel Nav1.3 has been implicated in peripheral neuropathic pain, we investigated its role in central neuropathic pain after SCI. In this study, adult male Sprague Dawley rats underwent T9 spinal contusion injury. Four weeks after injury when extracellular recordings demonstrated hyperexcitability of L3-L5 dorsal horn multireceptive nociceptive neurons, and when pain-related behaviors were evident, quantitative RT-PCR,in situhybridization, and immunocytochemistry revealed an upregulation of Nav1.3 in dorsal horn nociceptive neurons. Intrathecal administration of antisense oligodeoxynucleotides (ODNs) targeting Nav1.3 resulted in decreased expression of Nav1.3 mRNA and protein, reduced hyperexcitability of multireceptive dorsal horn neurons, and attenuated mechanical allodynia and thermal hyperalgesia after SCI. Expression of Nav1.3 protein and hyperexcitability in dorsal horn neurons as well as pain-related behaviors returned after cessation of antisense delivery. Responses to normally noxious stimuli and motor function were unchanged in SCI animals administered Nav1.3 antisense, and administration of mismatch ODNs had no effect. These results demonstrate for the first time that Nav1.3 is upregulated in second-order dorsal horn sensory neurons after nervous system injury, showing that SCI can trigger changes in sodium channel expression, and suggest a functional link between Nav1.3 expression and neuronal hyperexcitability associated with central neuropathic pain.

Bibliography

Hains, B. C., Klein, J. P., Saab, C. Y., Craner, M. J., Black, J. A., & Waxman, S. G. (2003). Upregulation of Sodium Channel Nav1.3 and Functional Involvement in Neuronal Hyperexcitability Associated with Central Neuropathic Pain after Spinal Cord Injury. The Journal of Neuroscience, 23(26), 8881â8892.

Authors 6

Bryan C. Hains (first)
Joshua P. Klein (additional)
Carl Y. Saab (additional)
Matthew J. Craner (additional)
Joel A. Black (additional)
Stephen G. Waxman (additional)

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Dates

Type	When
Created	7 years, 4 months ago (April 12, 2018, 7:25 p.m.)
Deposited	2 years, 6 months ago (Feb. 23, 2023, 11:14 a.m.)
Indexed	2 months ago (July 1, 2025, 6:29 p.m.)
Issued	21 years, 11 months ago (Oct. 1, 2003)
Published	21 years, 11 months ago (Oct. 1, 2003)
Published Online	21 years, 11 months ago (Oct. 1, 2003)
Published Print	21 years, 11 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{Hains_2003, title={Upregulation of Sodium Channel Nav1.3 and Functional Involvement in Neuronal Hyperexcitability Associated with Central Neuropathic Pain after Spinal Cord Injury}, volume={23}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.23-26-08881.2003}, DOI={10.1523/jneurosci.23-26-08881.2003}, number={26}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Hains, Bryan C. and Klein, Joshua P. and Saab, Carl Y. and Craner, Matthew J. and Black, Joel A. and Waxman, Stephen G.}, year={2003}, month=oct, pages={8881–8892} }

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