DOI: 10.1523/jneurosci.23-03-01006.2003. Molecular Evidence for the Functional Role of Dopamine D<sub>3</sub>Receptor in the Morphine-Induced Rewarding Effect and Hyperlocomotion

Molecular Evidence for the Functional Role of Dopamine D3Receptor in the Morphine-Induced Rewarding Effect and Hyperlocomotion

10.1523/jneurosci.23-03-01006.2003

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

The aim of the present study was to investigate the role of dopamine D3receptors in the rewarding effect and hyperlocomotion induced by a prototypical μ-opioid receptor agonist morphine using dopamine D3receptor knock-out mice. The μ-opioid receptor in the brain determined by the [tylosil-3,5-3H(N)]-[d-Ala2,N-MePhe4,Gly-ol5]enkephalin binding assay was not significantly changed by a deletion of the dopamine D3receptor gene. Furthermore, we found that no significant differences in G-protein activation by morphine in the limbic forebrain and lower midbrain were noted between the two genotypes. These results suggest that the function of the μ-opioid receptor itself was not affected by a deletion of the dopamine D3receptor gene. To ascertain the morphine-induced rewarding effect in both genotypes, the conditioned place preference paradigm was performed. Deletion of the dopamine D3receptor gene resulted in a remarkable enhancement of the morphine-induced rewarding effect. Furthermore, knock-out mice with deletions of the dopamine D3receptor revealed a dramatic potentiation of morphine-induced hyperlocomotion. Under these conditions, a loss of the dopamine D3receptor gene had no effect on the basal levels of dopamine and the increased dopamine turnover by morphine in the limbic forebrain. These findings provide further evidence that dopamine D3receptor contributes to the postsynaptically negative modulation of the mesolimbic dopaminergic pathway that is associated with the rewarding effect and hyperlocomotion through the stimulation of μ-opioid receptors induced by morphine in the mouse.

Bibliography

Narita, M., Mizuo, K., Mizoguchi, H., Sakata, M., Narita, M., Tseng, L. F., & Suzuki, T. (2003). Molecular Evidence for the Functional Role of Dopamine D3Receptor in the Morphine-Induced Rewarding Effect and Hyperlocomotion. The Journal of Neuroscience, 23(3), 1006â1012.

Authors 7

Minoru Narita (first)
Keisuke Mizuo (additional)
Hirokazu Mizoguchi (additional)
Mamoru Sakata (additional)
Michiko Narita (additional)
Leon F. Tseng (additional)
Tsutomu Suzuki (additional)

References 41 Referenced 52

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Dates

Type	When
Created	7 years, 4 months ago (April 13, 2018, 6:30 p.m.)
Deposited	1 month, 3 weeks ago (July 3, 2025, 1:33 p.m.)
Indexed	1 month, 3 weeks ago (July 4, 2025, 12:11 a.m.)
Issued	22 years, 6 months ago (Feb. 1, 2003)
Published	22 years, 6 months ago (Feb. 1, 2003)
Published Online	22 years, 6 months ago (Feb. 1, 2003)
Published Print	22 years, 6 months ago (Feb. 1, 2003)

Funders 0

None

BibTeX

@article{Narita_2003, title={Molecular Evidence for the Functional Role of Dopamine D3Receptor in the Morphine-Induced Rewarding Effect and Hyperlocomotion}, volume={23}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.23-03-01006.2003}, DOI={10.1523/jneurosci.23-03-01006.2003}, number={3}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Narita, Minoru and Mizuo, Keisuke and Mizoguchi, Hirokazu and Sakata, Mamoru and Narita, Michiko and Tseng, Leon F. and Suzuki, Tsutomu}, year={2003}, month=feb, pages={1006–1012} }

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