DOI: 10.1523/jneurosci.21-14-05017.2001. Cyclin-Dependent Kinases and P53 Pathways Are Activated Independently and Mediate Bax Activation in Neurons after DNA Damage

Cyclin-Dependent Kinases and P53 Pathways Are Activated Independently and Mediate Bax Activation in Neurons after DNA Damage

10.1523/jneurosci.21-14-05017.2001

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

DNA damage has been implicated as one important initiator of cell death in neuropathological conditions such as stroke. Accordingly, it is important to understand the signaling processes that control neuronal death induced by this stimulus. Previous evidence has shown that the death of embryonic cortical neurons treated with the DNA-damaging agent camptothecin is dependent on the tumor suppressor p53 and cyclin-dependent kinase (CDK) activity and that the inhibition of either pathway alone leads to enhanced and prolonged survival. We presently show that p53 and CDKs are activated independently on parallel pathways. An increase in p53 protein levels, nuclear localization, and DNA binding that result from DNA damage are not affected by the inhibition of CDK activity. Conversely, no decrease in retinoblastoma protein (pRb) phosphorylation was observed in p53-deficient neurons that were treated with camptothecin. However, either p53 deficiency or the inhibition of CDK activity alone inhibited Bax translocation, cytochromecrelease, and caspase-3-like activation. Taken together, our results indicate that p53 and CDK are activated independently and then act in concert to control Bax-mediated apoptosis.

Bibliography

Morris, E. J., Keramaris, E., Rideout, H. J., Slack, R. S., Dyson, N. J., Stefanis, L., & Park, D. S. (2001). Cyclin-Dependent Kinases and P53 Pathways Are Activated Independently and Mediate Bax Activation in Neurons after DNA Damage. The Journal of Neuroscience, 21(14), 5017â5026.

Authors 7

Erick J. Morris (first)
Elizabeth Keramaris (additional)
Hardy J. Rideout (additional)
Ruth S. Slack (additional)
Nicholas J. Dyson (additional)
Leonidas Stefanis (additional)
David S. Park (additional)

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Dates

Type	When
Created	7 years, 4 months ago (April 13, 2018, 6:25 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 10:05 a.m.)
Indexed	1 month, 4 weeks ago (July 7, 2025, 1:46 a.m.)
Issued	24 years, 1 month ago (July 15, 2001)
Published	24 years, 1 month ago (July 15, 2001)
Published Online	24 years, 1 month ago (July 15, 2001)
Published Print	24 years, 1 month ago (July 15, 2001)

Funders 0

None

BibTeX

@article{Morris_2001, title={Cyclin-Dependent Kinases and P53 Pathways Are Activated Independently and Mediate Bax Activation in Neurons after DNA Damage}, volume={21}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.21-14-05017.2001}, DOI={10.1523/jneurosci.21-14-05017.2001}, number={14}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Morris, Erick J. and Keramaris, Elizabeth and Rideout, Hardy J. and Slack, Ruth S. and Dyson, Nicholas J. and Stefanis, Leonidas and Park, David S.}, year={2001}, month=jul, pages={5017–5026} }

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