DOI: 10.1523/jneurosci.21-04-01117.2001. Functional Expression of the New Gap Junction Gene Connexin47 Transcribed in Mouse Brain and Spinal Cord Neurons

Functional Expression of the New Gap Junction Gene Connexin47 Transcribed in Mouse Brain and Spinal Cord Neurons

10.1523/jneurosci.21-04-01117.2001

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

A new mouse gap junction gene that codes for a protein of 46,551 Da has been identified and designated connexin47 (Cx47). It mapped as a single-copy gene to mouse chromosome 11. In human HeLa cells andXenopusoocytes, expression of mouse Cx47 or a fusion protein of Cx47 and enhanced green fluorescent protein induced intercellular channels that displayed strong sensitivity to transjunctional voltage. Tracer injections in Cx47-transfected HeLa cells revealed intercellular diffusion of neurobiotin, Lucifer yellow, and 4′,6-diamidino-2-phenylindole. Recordings of single channels yielded a unitary conductance of 55 pS main state and 8 pS substate. Cx47 mRNA expression was high in spinal cord and brain but was not found in retina, liver, heart, and lung. A low level of Cx47 expression was detected in ovaries.In situhybridizations demonstrated high expression in α motor neurons of the spinal cord, pyramidal cells of the cortex and hippocampus, granular and molecular layers of the dentate gyrus, and Purkinje cells of the cerebellum as well as several nuclei of the brainstem. This expression pattern is distinct from, although partially overlapping with, that of the neuronally expressed connexin36 gene. Thus, electrical synapses in adult mammalian brain are likely to consist of different connexin proteins depending on the neuronal subtype.

Bibliography

Teubner, B., Odermatt, B., GÃ¼ldenagel, M., SÃ¶hl, G., Degen, J., Bukauskas, F. F., Kronengold, J., Verselis, V. K., Jung, Y. T., Kozak, C. A., Schilling, K., & Willecke, K. (2001). Functional Expression of the New Gap Junction Gene Connexin47 Transcribed in Mouse Brain and Spinal Cord Neurons. The Journal of Neuroscience, 21(4), 1117â1126.

Authors 12

Barbara Teubner (first)
Benjamin Odermatt (additional)
Martin Güldenagel (additional)
Goran Söhl (additional)
Joachim Degen (additional)
Feliksas F. Bukauskas (additional)
Jack Kronengold (additional)
Vytas K. Verselis (additional)
Yong Tae Jung (additional)
Christine A. Kozak (additional)
Karl Schilling (additional)
Klaus Willecke (additional)

References 52 Referenced 113

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{'key': '2023041302474063000_21.4.1117.51', 'first-page': '76', 'article-title': 'Biological functions of connexin genes revealed by human genetic defects, dominant negative approaches and targeted deletions in the mouse.', 'volume': '219', 'author': 'Willecke', 'year': '1999', 'journal-title': 'Novartis Found Symp'} / Novartis Found Symp / Biological functions of connexin genes revealed by human genetic defects, dominant negative approaches and targeted deletions in the mouse. by Willecke (1999)
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Dates

Type	When
Created	7 years, 5 months ago (April 4, 2018, 8:12 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 9:51 a.m.)
Indexed	4 months, 2 weeks ago (April 22, 2025, 4:12 a.m.)
Issued	24 years, 6 months ago (Feb. 15, 2001)
Published	24 years, 6 months ago (Feb. 15, 2001)
Published Online	24 years, 6 months ago (Feb. 15, 2001)
Published Print	24 years, 6 months ago (Feb. 15, 2001)

Funders 0

None

BibTeX

@article{Teubner_2001, title={Functional Expression of the New Gap Junction Gene Connexin47 Transcribed in Mouse Brain and Spinal Cord Neurons}, volume={21}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.21-04-01117.2001}, DOI={10.1523/jneurosci.21-04-01117.2001}, number={4}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Teubner, Barbara and Odermatt, Benjamin and Güldenagel, Martin and Söhl, Goran and Degen, Joachim and Bukauskas, Feliksas F. and Kronengold, Jack and Verselis, Vytas K. and Jung, Yong Tae and Kozak, Christine A. and Schilling, Karl and Willecke, Klaus}, year={2001}, month=feb, pages={1117–1126} }

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