DOI: 10.1523/jneurosci.21-01-00230.2001. Expression of Neuronal Connexin36 in AII Amacrine Cells of the Mammalian Retina

Expression of Neuronal Connexin36 in AII Amacrine Cells of the Mammalian Retina

10.1523/jneurosci.21-01-00230.2001

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

We have studied the expression pattern of neuronal connexin36 (Cx36) in the mouse and rat retina. In vertical sections of both retinas, a polyclonal antibody directed against Cx36 produced punctate labeling in the inner plexiform layer (IPL). Intense immunoreactivity was localized to the entire OFF sublamina of the IPL, and much weaker staining could be observed in the ON sublamina. Double-labeling experiments in the rat retina with antibodies directed against parvalbumin indicate that Cx36 is expressed on dendrites of AII amacrine cells. Cx36-like immunoreactivity in sublamina a of the IPL did not overlap with lobular appendages or cell bodies of AII amacrine cells. In a mouse retinal slice preparation, AII amacrine and ON cone bipolar cells were intracellularly injected with Neurobiotin and counterstained with antibody against Cx36. Punctate labeling appeared to be in register with dendritic arborization of AII amacrines and cone bipolar cells in the ON sublamina of the IPL. Whereas AII amacrine cells isolated from the rat retina clearly displayed Cx36-like immunoreactivity, isolated ON cone bipolar cells were negative for Cx36. Axon terminals of rod bipolar cells were decorated with Cx36-positive contacts but did not express Cx36 themselves.These results indicate that Cx36 is expressed by AII amacrine cells in homologous and heterologous gap junctions made with AII amacrines and cone bipolar cells, respectively. The heterologous gap junctions appear to be heterotypic, because ON cone bipolar cells do not express Cx36.

Bibliography

Feigenspan, A., Teubner, B., Willecke, K., & Weiler, R. (2001). Expression of Neuronal Connexin36 in AII Amacrine Cells of the Mammalian Retina. The Journal of Neuroscience, 21(1), 230â239.

Authors 4

Andreas Feigenspan (first)
Barbara Teubner (additional)
Klaus Willecke (additional)
Reto Weiler (additional)

References 54 Referenced 171

10.1002/(SICI)1097-4547(20000315)59:6<813::AID-JNR14>3.0.CO;2-#
10.1007/BF01868459
10.1002/bies.950180906
10.1002/cne.903320404
10.1046/j.1460-9568.1998.00163.x
10.1002/jemt.1070310510
10.1523/JNEUROSCI.06-02-00331.1986
10.1074/jbc.271.30.17903
10.1002/(SICI)1098-1136(199809)24:1<1::AID-GLIA1>3.0.CO;2-A / Glia / From glue (“nervenkitt”) to glia: a prologue. by Dermietzel (1998)
10.1038/28184 / Nature / Electrical coupling underlies high frequency oscillations in the hippocampus in vitro. by Draguhn (1999)
10.1002/cne.903610310
10.1016/0006-8993(75)90983-X
10.1038/47029
10.1146/annurev.bi.65.070196.002355
10.1002/cne.903010308
10.1002/1096-9861(20000918)425:2<193::AID-CNE3>3.0.CO;2-N
10.1016/S0896-6273(00)80313-X
10.1016/S0021-9258(18)46052-8 / J Biol Chem / Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping. by Haefliger (1992)
10.1523/JNEUROSCI.12-12-04911.1992
10.1002/(SICI)1096-9861(19980706)396:3<310::AID-CNE3>3.0.CO;2-5
10.1007/BF01236124
10.1126/science.186.4158.47
10.1002/(SICI)1096-9861(19991018)413:2<305::AID-CNE10>3.0.CO;2-E
10.1002/(SICI)1096-9861(19960226)366:1<15::AID-CNE2>3.0.CO;2-N
10.1017/S0952523800003175
10.1038/377734a0
10.1016/0304-3940(88)90025-0
10.1152/jn.1982.47.5.928 / J Neurophysiol / AII amacrine cells quicken time course of rod signals in the cat retina. by Nelson (1982)
10.1091/mbc.7.2.233
10.1523/JNEUROSCI.18-19-07625.1998
10.1098/rspb.1980.0060 / Proc R Soc Lond B Biol Sci / Amacrine cells, displaced amacrine cells and interplexiform cells in the retina of the rat. by Perry (1980)
10.1523/JNEUROSCI.14-08-05131.1994
10.1016/S0014-5793(98)00479-7
10.1523/JNEUROSCI.19-22-09848.1999
10.1038/377676a0 / Nature / Tuning retinal circuits. by Sterling (1995)
10.1073/pnas.93.25.14906
10.1002/cne.903250203
10.1002/cne.903470111
10.1038/73936
10.1007/s002320001094
10.1016/0304-3940(91)90024-N
10.1016/1350-9462(94)90014-0 / Prog Retin Eye Res / Patterns of neuronal coupling in the retina. by Vaney (1994)
Vaney DI (1996) Cell coupling in the retina. in Gap junctions in the nervous system, eds Spray DC Dermietzel R (Landes, Austin, TX), pp 79–102. (10.1007/978-3-662-21935-5_5)
{'key': '2023041302471838000_21.1.230.44', 'first-page': '267', 'article-title': 'Neuronal coupling in rod signal pathways of the retina.', 'volume': '38', 'author': 'Vaney', 'year': '1997', 'journal-title': 'Invest Ophthalmol Vis Sci'} / Invest Ophthalmol Vis Sci / Neuronal coupling in rod signal pathways of the retina. by Vaney (1997)
Vaney DI (1999) Neuronal coupling in the central nervous system: lessons from the retina. Gap junction mediated intercellular signalling in health and disease (Wiley, Chichester, UK), pp 113–133. (10.1002/9780470515587.ch8)
10.1002/cne.903100203
10.1523/JNEUROSCI.07-12-04115.1987
10.1002/cne.903320403
Weiler R (1996) The modulation of gap junction permeability in the retina. in Gap junctions in the nervous system, eds Spray DC Dermietzel R (Landes, Austin, TX), pp 103–122. (10.1007/978-3-662-21935-5_6)
Weiler R, Feigenspan A, Teubner B, Willecke K (2000a) Cellular localization of the murine connexin Cx36 in the mammalian retina. ARVO Abstr: B387.
10.1016/S0165-0173(99)00071-5
10.1091/mbc.3.7.711
10.1046/j.1460-9568.1999.00607.x
{'key': '2023041302471838000_21.1.230.54', 'first-page': '1', 'article-title': 'The diversity of connexin genes encoding gap junctional proteins.', 'volume': '56', 'author': 'Willecke', 'year': '1991', 'journal-title': 'Eur J Cell Biol'} / Eur J Cell Biol / The diversity of connexin genes encoding gap junctional proteins. by Willecke (1991)

Dates

Type	When
Created	7 years, 5 months ago (April 5, 2018, 6:33 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 9:44 a.m.)
Indexed	1 month ago (July 30, 2025, 11:30 a.m.)
Issued	24 years, 8 months ago (Jan. 1, 2001)
Published	24 years, 8 months ago (Jan. 1, 2001)
Published Online	24 years, 8 months ago (Jan. 1, 2001)
Published Print	24 years, 8 months ago (Jan. 1, 2001)

Funders 0

None

BibTeX

@article{Feigenspan_2001, title={Expression of Neuronal Connexin36 in AII Amacrine Cells of the Mammalian Retina}, volume={21}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.21-01-00230.2001}, DOI={10.1523/jneurosci.21-01-00230.2001}, number={1}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Feigenspan, Andreas and Teubner, Barbara and Willecke, Klaus and Weiler, Reto}, year={2001}, month=jan, pages={230–239} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        30
      ]
    ],
    "date-time": "2025-07-30T15:30:16Z",
    "timestamp": 1753889416737
  },
  "reference-count": 54,
  "publisher": "Society for Neuroscience",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            7,
            1
          ]
        ],
        "date-time": "2001-07-01T00:00:00Z",
        "timestamp": 993945600000
      },
      "content-version": "vor",
      "delay-in-days": 181,
      "URL": "https://creativecommons.org/licenses/by-nc-sa/4.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        1,
        1
      ]
    ]
  },
  "abstract": "<jats:p>We have studied the expression pattern of neuronal connexin36 (Cx36) in the mouse and rat retina. In vertical sections of both retinas, a polyclonal antibody directed against Cx36 produced punctate labeling in the inner plexiform layer (IPL). Intense immunoreactivity was localized to the entire OFF sublamina of the IPL, and much weaker staining could be observed in the ON sublamina. Double-labeling experiments in the rat retina with antibodies directed against parvalbumin indicate that Cx36 is expressed on dendrites of AII amacrine cells. Cx36-like immunoreactivity in sublamina a of the IPL did not overlap with lobular appendages or cell bodies of AII amacrine cells. In a mouse retinal slice preparation, AII amacrine and ON cone bipolar cells were intracellularly injected with Neurobiotin and counterstained with antibody against Cx36. Punctate labeling appeared to be in register with dendritic arborization of AII amacrines and cone bipolar cells in the ON sublamina of the IPL. Whereas AII amacrine cells isolated from the rat retina clearly displayed Cx36-like immunoreactivity, isolated ON cone bipolar cells were negative for Cx36. Axon terminals of rod bipolar cells were decorated with Cx36-positive contacts but did not express Cx36 themselves.</jats:p><jats:p>These results indicate that Cx36 is expressed by AII amacrine cells in homologous and heterologous gap junctions made with AII amacrines and cone bipolar cells, respectively. The heterologous gap junctions appear to be heterotypic, because ON cone bipolar cells do not express Cx36.</jats:p>",
  "DOI": "10.1523/jneurosci.21-01-00230.2001",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2018,
        4,
        5
      ]
    ],
    "date-time": "2018-04-05T22:33:47Z",
    "timestamp": 1522967627000
  },
  "page": "230-239",
  "source": "Crossref",
  "is-referenced-by-count": 171,
  "title": "Expression of Neuronal Connexin36 in AII Amacrine Cells of the Mammalian Retina",
  "prefix": "10.1523",
  "volume": "21",
  "author": [
    {
      "given": "Andreas",
      "family": "Feigenspan",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Barbara",
      "family": "Teubner",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Klaus",
      "family": "Willecke",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Reto",
      "family": "Weiler",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "393",
  "published-online": {
    "date-parts": [
      [
        2001,
        1,
        1
      ]
    ]
  },
  "reference": [
    {
      "key": "2023041302471838000_21.1.230.1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-4547(20000315)59:6<813::AID-JNR14>3.0.CO;2-#"
    },
    {
      "key": "2023041302471838000_21.1.230.2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01868459"
    },
    {
      "key": "2023041302471838000_21.1.230.3",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bies.950180906"
    },
    {
      "key": "2023041302471838000_21.1.230.4",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.903320404"
    },
    {
      "key": "2023041302471838000_21.1.230.5",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1460-9568.1998.00163.x"
    },
    {
      "key": "2023041302471838000_21.1.230.6",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1070310510"
    },
    {
      "key": "2023041302471838000_21.1.230.7",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.06-02-00331.1986"
    },
    {
      "key": "2023041302471838000_21.1.230.8",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.30.17903"
    },
    {
      "key": "2023041302471838000_21.1.230.9",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1002/(SICI)1098-1136(199809)24:1<1::AID-GLIA1>3.0.CO;2-A",
      "article-title": "From glue (\u201cnervenkitt\u201d) to glia: a prologue.",
      "volume": "4",
      "author": "Dermietzel",
      "year": "1998",
      "journal-title": "Glia"
    },
    {
      "key": "2023041302471838000_21.1.230.10",
      "doi-asserted-by": "crossref",
      "first-page": "189",
      "DOI": "10.1038/28184",
      "article-title": "Electrical coupling underlies high frequency oscillations in the hippocampus in vitro.",
      "volume": "394",
      "author": "Draguhn",
      "year": "1999",
      "journal-title": "Nature"
    },
    {
      "key": "2023041302471838000_21.1.230.11",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.903610310"
    },
    {
      "key": "2023041302471838000_21.1.230.12",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-8993(75)90983-X"
    },
    {
      "key": "2023041302471838000_21.1.230.13",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/47029"
    },
    {
      "key": "2023041302471838000_21.1.230.14",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.65.070196.002355"
    },
    {
      "key": "2023041302471838000_21.1.230.15",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.903010308"
    },
    {
      "key": "2023041302471838000_21.1.230.16",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/1096-9861(20000918)425:2<193::AID-CNE3>3.0.CO;2-N"
    },
    {
      "key": "2023041302471838000_21.1.230.17",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80313-X"
    },
    {
      "key": "2023041302471838000_21.1.230.18",
      "doi-asserted-by": "crossref",
      "first-page": "2057",
      "DOI": "10.1016/S0021-9258(18)46052-8",
      "article-title": "Four novel members of the connexin family of gap junction proteins. Molecular cloning, expression, and chromosome mapping.",
      "volume": "267",
      "author": "Haefliger",
      "year": "1992",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "2023041302471838000_21.1.230.19",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.12-12-04911.1992"
    },
    {
      "key": "2023041302471838000_21.1.230.20",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1096-9861(19980706)396:3<310::AID-CNE3>3.0.CO;2-5"
    },
    {
      "key": "2023041302471838000_21.1.230.21",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01236124"
    },
    {
      "key": "2023041302471838000_21.1.230.22",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.186.4158.47"
    },
    {
      "key": "2023041302471838000_21.1.230.23",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1096-9861(19991018)413:2<305::AID-CNE10>3.0.CO;2-E"
    },
    {
      "key": "2023041302471838000_21.1.230.24",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1096-9861(19960226)366:1<15::AID-CNE2>3.0.CO;2-N"
    },
    {
      "key": "2023041302471838000_21.1.230.25",
      "doi-asserted-by": "publisher",
      "DOI": "10.1017/S0952523800003175"
    },
    {
      "key": "2023041302471838000_21.1.230.26",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/377734a0"
    },
    {
      "key": "2023041302471838000_21.1.230.27",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(88)90025-0"
    },
    {
      "key": "2023041302471838000_21.1.230.28",
      "doi-asserted-by": "crossref",
      "first-page": "928",
      "DOI": "10.1152/jn.1982.47.5.928",
      "article-title": "AII amacrine cells quicken time course of rod signals in the cat retina.",
      "volume": "47",
      "author": "Nelson",
      "year": "1982",
      "journal-title": "J Neurophysiol"
    },
    {
      "key": "2023041302471838000_21.1.230.29",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.7.2.233"
    },
    {
      "key": "2023041302471838000_21.1.230.30",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.18-19-07625.1998"
    },
    {
      "key": "2023041302471838000_21.1.230.31",
      "doi-asserted-by": "crossref",
      "first-page": "415",
      "DOI": "10.1098/rspb.1980.0060",
      "article-title": "Amacrine cells, displaced amacrine cells and interplexiform cells in the retina of the rat.",
      "volume": "208",
      "author": "Perry",
      "year": "1980",
      "journal-title": "Proc R Soc Lond B Biol Sci"
    },
    {
      "key": "2023041302471838000_21.1.230.32",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.14-08-05131.1994"
    },
    {
      "key": "2023041302471838000_21.1.230.33",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-5793(98)00479-7"
    },
    {
      "key": "2023041302471838000_21.1.230.34",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.19-22-09848.1999"
    },
    {
      "key": "2023041302471838000_21.1.230.35",
      "doi-asserted-by": "crossref",
      "first-page": "676",
      "DOI": "10.1038/377676a0",
      "article-title": "Tuning retinal circuits.",
      "volume": "377",
      "author": "Sterling",
      "year": "1995",
      "journal-title": "Nature"
    },
    {
      "key": "2023041302471838000_21.1.230.36",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.25.14906"
    },
    {
      "key": "2023041302471838000_21.1.230.37",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.903250203"
    },
    {
      "key": "2023041302471838000_21.1.230.38",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.903470111"
    },
    {
      "key": "2023041302471838000_21.1.230.39",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/73936"
    },
    {
      "key": "2023041302471838000_21.1.230.40",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s002320001094"
    },
    {
      "key": "2023041302471838000_21.1.230.41",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-3940(91)90024-N"
    },
    {
      "key": "2023041302471838000_21.1.230.42",
      "doi-asserted-by": "crossref",
      "first-page": "302",
      "DOI": "10.1016/1350-9462(94)90014-0",
      "article-title": "Patterns of neuronal coupling in the retina.",
      "volume": "13",
      "author": "Vaney",
      "year": "1994",
      "journal-title": "Prog Retin Eye Res"
    },
    {
      "key": "2023041302471838000_21.1.230.43",
      "doi-asserted-by": "crossref",
      "unstructured": "Vaney DI (1996) Cell coupling in the retina. in Gap junctions in the nervous system, eds Spray DC Dermietzel R (Landes, Austin, TX), pp 79\u2013102.",
      "DOI": "10.1007/978-3-662-21935-5_5"
    },
    {
      "key": "2023041302471838000_21.1.230.44",
      "first-page": "267",
      "article-title": "Neuronal coupling in rod signal pathways of the retina.",
      "volume": "38",
      "author": "Vaney",
      "year": "1997",
      "journal-title": "Invest Ophthalmol Vis Sci"
    },
    {
      "key": "2023041302471838000_21.1.230.45",
      "doi-asserted-by": "crossref",
      "unstructured": "Vaney DI (1999) Neuronal coupling in the central nervous system: lessons from the retina. Gap junction mediated intercellular signalling in health and disease (Wiley, Chichester, UK), pp 113\u2013133.",
      "DOI": "10.1002/9780470515587.ch8"
    },
    {
      "key": "2023041302471838000_21.1.230.46",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.903100203"
    },
    {
      "key": "2023041302471838000_21.1.230.47",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.07-12-04115.1987"
    },
    {
      "key": "2023041302471838000_21.1.230.48",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cne.903320403"
    },
    {
      "key": "2023041302471838000_21.1.230.49",
      "doi-asserted-by": "crossref",
      "unstructured": "Weiler R (1996) The modulation of gap junction permeability in the retina. in Gap junctions in the nervous system, eds Spray DC Dermietzel R (Landes, Austin, TX), pp 103\u2013122.",
      "DOI": "10.1007/978-3-662-21935-5_6"
    },
    {
      "key": "2023041302471838000_21.1.230.50",
      "unstructured": "Weiler R, Feigenspan A, Teubner B, Willecke K (2000a) Cellular localization of the murine connexin Cx36 in the mammalian retina. ARVO Abstr: B387."
    },
    {
      "key": "2023041302471838000_21.1.230.51",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0165-0173(99)00071-5"
    },
    {
      "key": "2023041302471838000_21.1.230.52",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.3.7.711"
    },
    {
      "key": "2023041302471838000_21.1.230.53",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1460-9568.1999.00607.x"
    },
    {
      "key": "2023041302471838000_21.1.230.54",
      "first-page": "1",
      "article-title": "The diversity of connexin genes encoding gap junctional proteins.",
      "volume": "56",
      "author": "Willecke",
      "year": "1991",
      "journal-title": "Eur J Cell Biol"
    }
  ],
  "container-title": "The Journal of Neuroscience",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://syndication.highwire.org/content/doi/10.1523/JNEUROSCI.21-01-00230.2001",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        4,
        13
      ]
    ],
    "date-time": "2023-04-13T13:44:30Z",
    "timestamp": 1681393470000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.21-01-00230.2001"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        1,
        1
      ]
    ]
  },
  "references-count": 54,
  "journal-issue": {
    "issue": "1",
    "published-online": {
      "date-parts": [
        [
          2001,
          1,
          1
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          2001,
          1,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1523/JNEUROSCI.21-01-00230.2001"
  ],
  "URL": "http://dx.doi.org/10.1523/JNEUROSCI.21-01-00230.2001",
  "relation": {},
  "ISSN": [
    "0270-6474",
    "1529-2401"
  ],
  "subject": [],
  "container-title-short": "J. Neurosci.",
  "published": {
    "date-parts": [
      [
        2001,
        1,
        1
      ]
    ]
  }
}