DOI: 10.1523/jneurosci.20-08-02944.2000. Learning Performance of Normal and Mutant<i>Drosophila</i>after Repeated Conditioning Trials with Discrete Stimuli

Learning Performance of Normal and MutantDrosophilaafter Repeated Conditioning Trials with Discrete Stimuli

10.1523/jneurosci.20-08-02944.2000

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

A new olfactory conditioning procedure is described using short training trials with discrete presentation of conditioned stimuli (CS) and unconditioned stimuli (US). A short odor presentation along with a single-shock stimulus produced modest but reliable and reproducible learning. Multiple trials presented sequentially improved performance with increasing trial number. Trial spacing had a significant impact on performance. Two trials presented with a short intertrial interval (ITI) produced no improvement over a single trial; two trials with a 15 min ITI significantly boosted performance. This effect required two associative trials, because substituting one of the trials with the CS alone, US alone, or an unpaired CS–US failed to boost performance. The increase in initial performance with two trials decayed within 15 min after training. Thus, the effect is short-lived. The utility of using a battery of tests, including a single short trial, two massed trials, and two spaced trials, to investigate parameters of memory formation in several mutants was demonstrated.

Bibliography

Beck, C. D. O., Schroeder, B., & Davis, R. L. (2000). Learning Performance of Normal and MutantDrosophilaafter Repeated Conditioning Trials with Discrete Stimuli. The Journal of Neuroscience, 20(8), 2944â2953.

Authors 3

C. D. O. Beck (first)
Bradley Schroeder (additional)
Ronald L. Davis (additional)

References 37 Referenced 115

10.1038/289079a0
10.1016/S0896-6273(00)80016-1
10.1126/science.175.4020.451
{'key': '2023041302445278000_20.8.2944.4', 'first-page': '3599', 'article-title': 'Molecular analysis of cDNA clones and the corresponding genomic coding region of the Drosophila dunce+ locus, the structure gene for cAMP phosphodiesterase.', 'volume': '86', 'author': 'Chen', 'year': '1986', 'journal-title': 'Proc Nat Acad Sci USA'} / Proc Nat Acad Sci USA / Molecular analysis of cDNA clones and the corresponding genomic coding region of the Drosophila dunce+ locus, the structure gene for cAMP phosphodiesterase. by Chen (1986)
10.1126/science.280.5360.77
10.1016/0896-6273(93)90178-T
10.1152/physrev.1996.76.2.299 / Physiol Rev / Physiology and biochemistry of Drosophila learning mutants. by Davis (1996)
10.1083/jcb.90.1.101
10.1126/science.8303280
10.1073/pnas.73.5.1684
10.1037/h0070025
10.1146/annurev.psych.49.1.585
10.1016/0896-6273(93)90337-Q
10.1038/35079
10.1523/JNEUROSCI.15-03-01617.1995
10.1016/0896-6273(92)90026-A
10.1006/nlme.1998.3858
Jellies JA (1981) Associative olfactory conditioning in Drosophila melanogaster and memory retention through metamorphosis. Master thesis (Illinois State University).
10.1016/S0960-9822(06)00022-4
10.1016/0092-8674(92)90185-F
10.1016/0092-8674(84)90316-7 / Cell / Loss of calcium/calmodulin responsiveness in adenylate cyclase of rutabaga, a Drosophila learning mutant. by Livingstone (1984)
10.1016/S0166-4328(97)02307-3
10.1016/S0092-8674(00)80484-5
10.1016/S0896-6273(00)80673-X
10.1126/science.3775383
10.1037/0735-7044.113.1.103
10.1016/0896-6273(91)90253-V
10.1523/JNEUROSCI.18-20-08534.1998
10.1146/annurev.ne.11.030188.001553
10.1016/S0896-6273(00)80224-X
10.1016/0896-6273(93)90178-T
10.1037/0882-7974.11.4.607
10.1007/BF01350033
10.1016/0092-8674(94)90398-0
10.1037/0033-2909.108.3.442
10.1101/lm.5.1.166
{'key': '2023041302445278000_20.8.2944.37', 'first-page': '123', 'article-title': 'Trial spacing and trial distribution effects in Pavlovian conditioning: contributions of a comparator mechanism.', 'volume': '20', 'author': 'Yin', 'year': '1994', 'journal-title': 'J Exp Psychol'} / J Exp Psychol / Trial spacing and trial distribution effects in Pavlovian conditioning: contributions of a comparator mechanism. by Yin (1994)

Dates

Type	When
Created	7 years, 4 months ago (April 3, 2018, 1:31 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 9:16 a.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 7:50 a.m.)
Issued	25 years, 4 months ago (April 15, 2000)
Published	25 years, 4 months ago (April 15, 2000)
Published Online	25 years, 4 months ago (April 15, 2000)
Published Print	25 years, 4 months ago (April 15, 2000)

Funders 0

None

BibTeX

@article{Beck_2000, title={Learning Performance of Normal and MutantDrosophilaafter Repeated Conditioning Trials with Discrete Stimuli}, volume={20}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.20-08-02944.2000}, DOI={10.1523/jneurosci.20-08-02944.2000}, number={8}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Beck, C. D. O. and Schroeder, Bradley and Davis, Ronald L.}, year={2000}, month=apr, pages={2944–2953} }

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