Thalamic-Evoked Synaptic Interactions in Barrel Cortex Revealed by Optical Imaging
10.1523/jneurosci.20-04-01529.2000
Crossref journal-article
Society for Neuroscience
The Journal of Neuroscience (393)
Abstract

We used optical imaging of voltage-sensitive dye signals to study the spatiotemporal spread of activity in the mouse barrel cortex, evoked by stimulation of thalamocortical afferents in anin vitroslice preparation. Stimulation of the thalamus, at low current intensity, results in activity largely restricted to a single barrel, and to the border between layers Vb and VI. Low concentrations of the GABAAreceptor antagonist bicuculline increase the amplitude of the optical signals, without affecting their spatiotemporal propagation. Higher concentrations of bicuculline result in paroxysmal activity, which propagates via intracolumnar and intercolumnar excitatory pathways. Enhancing the activity of NMDA receptors, by removing Mg2+from the extracellular solution, dramatically alters the spatiotemporal pattern of excitation: activity spreads to supragranular and infragranular layers and adjacent barrel columns. This enhanced propagation is suppressed by the NMDA receptor antagonist AP5. A similar enhancement of activity propagation can be produced by stimulating the thalamus with a short, high-frequency pulse train. Application of AP5 suppresses the frequency-dependent spread of activity. These findings indicate that the spatiotemporal spread of activity in the barrel cortex is altered by varying the temporal patterns of thalamic inputs, via an NMDA receptor-mediated mechanism, and suggest that a similar process occurs during repetitive whisking activity.

Bibliography

Laaris, N., Carlson, G. C., & Keller, A. (2000). Thalamic-Evoked Synaptic Interactions in Barrel Cortex Revealed by Optical Imaging. The Journal of Neuroscience, 20(4), 1529–1537.

Authors 3
  1. Nora Laaris (first)
  2. Greg C. Carlson (additional)
  3. Asaf Keller (additional)
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Dates
Type When
Created 7 years, 4 months ago (April 3, 2018, 1:33 p.m.)
Deposited 2 years, 4 months ago (April 13, 2023, 9:12 a.m.)
Indexed 2 months, 3 weeks ago (June 11, 2025, 4:05 p.m.)
Issued 25 years, 6 months ago (Feb. 15, 2000)
Published 25 years, 6 months ago (Feb. 15, 2000)
Published Online 25 years, 6 months ago (Feb. 15, 2000)
Published Print 25 years, 6 months ago (Feb. 15, 2000)
Funders 0

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@article{Laaris_2000, title={Thalamic-Evoked Synaptic Interactions in Barrel Cortex Revealed by Optical Imaging}, volume={20}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.20-04-01529.2000}, DOI={10.1523/jneurosci.20-04-01529.2000}, number={4}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Laaris, Nora and Carlson, Greg C. and Keller, Asaf}, year={2000}, month=feb, pages={1529–1537} }