DOI: 10.1523/jneurosci.20-02-00709.2000. A Highly Conserved Enhancer in the<i>Dlx5/Dlx6</i>Intergenic Region is the Site of Cross-Regulatory Interactions between<i>Dlx</i>Genes in the Embryonic Forebrain

A Highly Conserved Enhancer in theDlx5/Dlx6Intergenic Region is the Site of Cross-Regulatory Interactions betweenDlxGenes in the Embryonic Forebrain

10.1523/jneurosci.20-02-00709.2000

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

FourDlxhomeobox genes,Dlx1,Dlx2,Dlx5, andDlx6are expressed in the same primordia of the mouse forebrain with temporally overlapping patterns. The four genes are organized as two tail-to-tail pairs,Dlx1/Dlx2andDlx5/Dlx6, a genomic arrangement conserved in distantly related vertebrates like zebrafish. TheDlx5/Dlx6intergenic region contains two sequences of a few hundred base pairs, remarkably well conserved between mouse and zebrafish. Reporter transgenes containing these two sequences are expressed in the forebrain of transgenic mice and zebrafish with patterns highly similar to endogenousDlx5andDlx6expression. The activity of the transgene is drastically reduced in mouse mutants lacking bothDlx1andDlx2,consistent with the decrease in endogenousDlx5andDlx6expression. These results suggest that cross-regulation by Dlx proteins, mediated by the intergenic sequences, is essential forDlx5andDlx6expression in the forebrain. This hypothesis is supported by cotransfection and DNA-protein binding experiments. We propose that theDlxgenes are part of a highly conserved developmental pathway that regulates forebrain development.

Bibliography

Zerucha, T., StÃ¼hmer, T., Hatch, G., Park, B. K., Long, Q., Yu, G., Gambarotta, A., Schultz, J. R., Rubenstein, J. L. R., & Ekker, M. (2000). A Highly Conserved Enhancer in theDlx5/Dlx6Intergenic Region is the Site of Cross-Regulatory Interactions betweenDlxGenes in the Embryonic Forebrain. The Journal of Neuroscience, 20(2), 709â721.

Authors 10

Ted Zerucha (first)
Thorsten Stühmer (additional)
Gary Hatch (additional)
Byung K. Park (additional)
Qiaoming Long (additional)
Guoying Yu (additional)
Adrianna Gambarotta (additional)
Joshua R. Schultz (additional)
John L. R. Rubenstein (additional)
Marc Ekker (additional)

References 45 Referenced 293

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Dates

Type	When
Created	7 years, 5 months ago (April 5, 2018, 6:34 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 9:04 a.m.)
Indexed	2 days, 10 hours ago (Sept. 4, 2025, 9:21 a.m.)
Issued	25 years, 7 months ago (Jan. 15, 2000)
Published	25 years, 7 months ago (Jan. 15, 2000)
Published Online	25 years, 7 months ago (Jan. 15, 2000)
Published Print	25 years, 7 months ago (Jan. 15, 2000)

Funders 0

None

BibTeX

@article{Zerucha_2000, title={A Highly Conserved Enhancer in theDlx5/Dlx6Intergenic Region is the Site of Cross-Regulatory Interactions betweenDlxGenes in the Embryonic Forebrain}, volume={20}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.20-02-00709.2000}, DOI={10.1523/jneurosci.20-02-00709.2000}, number={2}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Zerucha, Ted and Stühmer, Thorsten and Hatch, Gary and Park, Byung K. and Long, Qiaoming and Yu, Guoying and Gambarotta, Adrianna and Schultz, Joshua R. and Rubenstein, John L. R. and Ekker, Marc}, year={2000}, month=jan, pages={709–721} }

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    ]
  }
}