DOI: 10.1523/jneurosci.19-21-09445.1999. Differentiation of Mammalian Vestibular Hair Cells from Conditionally Immortal, Postnatal Supporting Cells

Differentiation of Mammalian Vestibular Hair Cells from Conditionally Immortal, Postnatal Supporting Cells

10.1523/jneurosci.19-21-09445.1999

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

We provide evidence from a newly established, conditionally immortal cell line (UB/UE-1) that vestibular supporting cells from the mammalian inner ear can differentiate postnatally into more than one variant of hair cell. A clonal supporting cell line was established from pure utricular sensory epithelia of H2kbtsA58 transgenic mice 2 d after birth. Cell proliferation was dependent on conditional expression of the immortalizing gene, the “T” antigen from the SV40 virus. Proliferating cells expressed cytokeratins, and patch-clamp recordings revealed that they all expressed small membrane currents with little time-dependence. They stopped dividing within 2 d of being transferred to differentiating conditions, and within a week they formed three defined populations expressing membrane currents characteristic of supporting cells and two kinds of neonatal hair cell. The cells expressed several characteristic features of normal hair cells, including the transcription factor Brn3.1, a functional acetylcholine receptor composed of α9 subunits, and the cytoskeletal proteins myosin VI, myosin VIIa, and fimbrin. Immunofluorescence labeling and electron microscopy showed that the cells formed complex cytoskeletal arrays on their upper surfaces with structural features resembling those at the apices of normal hair cells. The cell line UB/UE-1 provides a valuablein vitropreparation in which the expression of numerous structural and physiological components can be initiated or upregulated during early stages of mammalian hair cell commitment and differentiation.

Bibliography

Lawlor, P., Marcotti, W., Rivolta, M. N., Kros, C. J., & Holley, M. C. (1999). Differentiation of Mammalian Vestibular Hair Cells from Conditionally Immortal, Postnatal Supporting Cells. The Journal of Neuroscience, 19(21), 9445â9458.

Authors 5

Patrick Lawlor (first)
Walter Marcotti (additional)
Marcelo N. Rivolta (additional)
Corné J. Kros (additional)
Matthew C. Holley (additional)

References 56 Referenced 41

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Dates

Type	When
Created	7 years, 5 months ago (April 3, 2018, 10:20 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 8:59 a.m.)
Indexed	1 year, 3 months ago (May 17, 2024, 9:39 a.m.)
Issued	25 years, 10 months ago (Nov. 1, 1999)
Published	25 years, 10 months ago (Nov. 1, 1999)
Published Online	25 years, 10 months ago (Nov. 1, 1999)
Published Print	25 years, 10 months ago (Nov. 1, 1999)

Funders 0

None

BibTeX

@article{Lawlor_1999, title={Differentiation of Mammalian Vestibular Hair Cells from Conditionally Immortal, Postnatal Supporting Cells}, volume={19}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.19-21-09445.1999}, DOI={10.1523/jneurosci.19-21-09445.1999}, number={21}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Lawlor, Patrick and Marcotti, Walter and Rivolta, Marcelo N. and Kros, Corné J. and Holley, Matthew C.}, year={1999}, month=nov, pages={9445–9458} }

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