DOI: 10.1523/jneurosci.19-09-03629.1999. Cholinergic Modulation of Neostriatal Output: A Functional Antagonism between Different Types of Muscarinic Receptors

Cholinergic Modulation of Neostriatal Output: A Functional Antagonism between Different Types of Muscarinic Receptors

10.1523/jneurosci.19-09-03629.1999

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

It is demonstrated that acetylcholine released from cholinergic interneurons modulates the excitability of neostriatal projection neurons. Physostigmine and neostigmine increase input resistance (RN) and enhance evoked discharge of spiny projection neurons in a manner similar to muscarine. Muscarinic RNincrease occurs in the whole subthreshold voltage range (−100 to −45 mV), remains in the presence of TTX and Cd2+, and can be blocked by the relatively selective M1,4muscarinic receptor antagonist pirenzepine but not by M2or M3selective antagonists. Cs+occludes muscarinic effects at potentials more negative than −80 mV. A Na+reduction in the bath occludes muscarinic effects at potentials more positive than −70 mV. Thus, muscarinic effects involve different ionic conductances: inward rectifying and cationic. The relatively selective M2receptor antagonist AF-DX 116 does not block muscarinic effects on the projection neuron but, surprisingly, has the ability to mimic agonistic actions increasing RNand firing. Both effects are blocked by pirenzepine. HPLC measurements of acetylcholine demonstrate that AF-DX 116 but not pirenzepine greatly increases endogenous acetylcholine release in brain slices. Therefore, the effects of the M2antagonist on the projection neurons were attributable to autoreceptor block on cholinergic interneurons. These experiments show distinct opposite functions of muscarinic M1- and M2-type receptors in neostriatal output, i.e., the firing of projection neurons. The results suggest that the use of more selective antimuscarinics may be more profitable for the treatment of motor deficits.

Bibliography

Galarraga, E., HernÃ¡ndez-LÃ³pez, S., Reyes, A., Miranda, I., Bermudez-Rattoni, F., Vilchis, C., & Bargas, J. (1999). Cholinergic Modulation of Neostriatal Output: A Functional Antagonism between Different Types of Muscarinic Receptors. The Journal of Neuroscience, 19(9), 3629â3638.

Authors 7

Elvira Galarraga (first)
Salvador Hernández-López (additional)
Arturo Reyes (additional)
Isabel Miranda (additional)
Federico Bermudez-Rattoni (additional)
Carmen Vilchis (additional)
José Bargas (additional)

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Dates

Type	When
Created	7 years, 5 months ago (April 3, 2018, 8:45 a.m.)
Deposited	2 months ago (July 3, 2025, 7:15 a.m.)
Indexed	2 months ago (July 3, 2025, 7:40 a.m.)
Issued	26 years, 4 months ago (May 1, 1999)
Published	26 years, 4 months ago (May 1, 1999)
Published Online	26 years, 4 months ago (May 1, 1999)
Published Print	26 years, 4 months ago (May 1, 1999)

Funders 0

None

BibTeX

@article{Galarraga_1999, title={Cholinergic Modulation of Neostriatal Output: A Functional Antagonism between Different Types of Muscarinic Receptors}, volume={19}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.19-09-03629.1999}, DOI={10.1523/jneurosci.19-09-03629.1999}, number={9}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Galarraga, Elvira and Hernández-López, Salvador and Reyes, Arturo and Miranda, Isabel and Bermudez-Rattoni, Federico and Vilchis, Carmen and Bargas, José}, year={1999}, month=may, pages={3629–3638} }

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