DOI: 10.1523/jneurosci.19-04-01263.1999. Rapid, Activation-Induced Redistribution of Ionotropic Glutamate Receptors in Cultured Hippocampal Neurons

Rapid, Activation-Induced Redistribution of Ionotropic Glutamate Receptors in Cultured Hippocampal Neurons

10.1523/jneurosci.19-04-01263.1999

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

We have examined the membrane localization of an AMPA receptor subunit (GluR1) and an NMDA receptor subunit (NR1) endogenously expressed in primary cultures of rat hippocampal neurons. In unstimulated cultures, both GluR1 and NR1 subunits were concentrated in SV2-positive synaptic clusters associated with dendritic shafts and spines. Within 5 min after the addition of 100 μmglutamate to the culture medium, a rapid and selective redistribution of GluR1 subunits away from a subset of synaptic sites was observed. This redistribution of GluR1 subunits was also induced by AMPA, did not require NMDA receptor activation, did not result from ligand-induced neurotoxicity, and was reversible after the removal of agonist. The activation-induced redistribution of GluR1 subunits was associated with a pronounced (∼50%) decrease in the frequency of miniature EPSCs, consistent with a role of GluR1 subunit redistribution in mediating rapid regulation of synaptic efficacy. We conclude that ionotropic glutamate receptors are regulated in native neurons by rapid, subtype-specific membrane trafficking, which may modulate synaptic transmission in response to physiological or pathophysiological activation.

Bibliography

Lissin, D. V., Carroll, R. C., Nicoll, R. A., Malenka, R. C., & Zastrow, M. von. (1999). Rapid, Activation-Induced Redistribution of Ionotropic Glutamate Receptors in Cultured Hippocampal Neurons. The Journal of Neuroscience, 19(4), 1263â1272.

Authors 5

Dmitri V. Lissin (first)
Reed C. Carroll (additional)
Roger A. Nicoll (additional)
Robert C. Malenka (additional)
Mark von Zastrow (additional)

References 26 Referenced 175

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Dates

Type	When
Created	7 years, 4 months ago (April 4, 2018, 1:20 p.m.)
Deposited	1 month, 3 weeks ago (July 3, 2025, 8:40 a.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 8:21 a.m.)
Issued	26 years, 6 months ago (Feb. 15, 1999)
Published	26 years, 6 months ago (Feb. 15, 1999)
Published Online	26 years, 6 months ago (Feb. 15, 1999)
Published Print	26 years, 6 months ago (Feb. 15, 1999)

Funders 0

None

BibTeX

@article{Lissin_1999, title={Rapid, Activation-Induced Redistribution of Ionotropic Glutamate Receptors in Cultured Hippocampal Neurons}, volume={19}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.19-04-01263.1999}, DOI={10.1523/jneurosci.19-04-01263.1999}, number={4}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Lissin, Dmitri V. and Carroll, Reed C. and Nicoll, Roger A. and Malenka, Robert C. and Zastrow, Mark von}, year={1999}, month=feb, pages={1263–1272} }

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