DOI: 10.1523/jneurosci.18-01-00093.1998. Amphiphysin I Antisense Oligonucleotides Inhibit Neurite Outgrowth in Cultured Hippocampal Neurons

Amphiphysin I Antisense Oligonucleotides Inhibit Neurite Outgrowth in Cultured Hippocampal Neurons

10.1523/jneurosci.18-01-00093.1998

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

Amphiphysin I is an SH3 domain-containing neuronal protein, enriched in axon terminals, which was reported to act as a physiological binding partner for dynamin I in synaptic vesicle endocytosis. Rvs167 and Rvs161, the yeast homologs of amphiphysin I, have been implicated in endocytosis, actin function, and cell polarity. Now we have explored the possibility that amphiphysin I also may have a role in actin dynamics and cell polarity by testing the effect of amphiphysin I suppression on neurite outgrowth. Freshly plated hippocampal neurons were exposed to antisense oligonucleotides via a new delivery system based on a polycationic amphipathic polymer, PS980. Western blot analysis revealed that amphiphysin I levels steadily increased with neuronal differentiation, whereas in antisense-treated cultures amphiphysin I levels were reduced to ∼10% of control levels at 48 hr. Concomitantly, a collapse of growth cones and a severe inhibition of neurite outgrowth and axon formation were observed. A similar effect was observed previously after dynamin I suppression in the same culture system (Torre et al., 1994). We also have found that amphiphysin I and dynamin I colocalize in developing neurons at all developmental stages and that a pool of both proteins is colocalized with actin patches at the leading edge of growth cones. Our findings suggest a conserved role of the amphiphysin protein family in the dynamics of the cortical cell cytoskeleton and provide new evidence for a close functional link between amphiphysin I and dynamin I.

Bibliography

Mundigl, O., Ochoa, G.-C., David, C., Slepnev, V. I., Kabanov, A., & De Camilli, P. (1998). Amphiphysin I Antisense Oligonucleotides Inhibit Neurite Outgrowth in Cultured Hippocampal Neurons. The Journal of Neuroscience, 18(1), 93â103.

Authors 6

Olaf Mundigl (first)
Gian-Carlo Ochoa (additional)
Carol David (additional)
Vladimir I. Slepnev (additional)
Alexander Kabanov (additional)
Pietro De Camilli (additional)

References 61 Referenced 84

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Dates

Type	When
Created	7 years, 5 months ago (April 3, 2018, 11:06 a.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 7:49 a.m.)
Indexed	11 months, 4 weeks ago (Sept. 9, 2024, 3:17 a.m.)
Issued	27 years, 8 months ago (Jan. 1, 1998)
Published	27 years, 8 months ago (Jan. 1, 1998)
Published Online	27 years, 8 months ago (Jan. 1, 1998)
Published Print	27 years, 8 months ago (Jan. 1, 1998)

Funders 0

None

BibTeX

@article{Mundigl_1998, title={Amphiphysin I Antisense Oligonucleotides Inhibit Neurite Outgrowth in Cultured Hippocampal Neurons}, volume={18}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.18-01-00093.1998}, DOI={10.1523/jneurosci.18-01-00093.1998}, number={1}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Mundigl, Olaf and Ochoa, Gian-Carlo and David, Carol and Slepnev, Vladimir I. and Kabanov, Alexander and De Camilli, Pietro}, year={1998}, month=jan, pages={93–103} }

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