DOI: 10.1523/jneurosci.17-21-08061.1997. <i>Caenorhabditis elegans rab-3</i>Mutant Synapses Exhibit Impaired Function and Are Partially Depleted of Vesicles

Caenorhabditis elegans rab-3Mutant Synapses Exhibit Impaired Function and Are Partially Depleted of Vesicles

10.1523/jneurosci.17-21-08061.1997

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

Rab molecules regulate vesicular trafficking in many different exocytic and endocytic transport pathways in eukaryotic cells. In neurons, rab3 has been proposed to play a crucial role in regulating synaptic vesicle release. To elucidate the role of rab3 in synaptic transmission, we isolated and characterizedCaenorhabditis elegans rab-3mutants. Similar to the mouse rab3A mutants, these mutants survived and exhibited only mild behavioral abnormalities. In contrast to the mouse mutants, synaptic transmission was perturbed in these animals. Extracellular electrophysiological recordings revealed that synaptic transmission in the pharyngeal nervous system was impaired. Furthermore,rab-3animals were resistant to the acetylcholinesterase inhibitor aldicarb, suggesting that cholinergic transmission was generally depressed. Last, synaptic vesicle populations were redistributed inrab-3mutants. In motor neurons, vesicle populations at synapses were depleted to 40% of normal levels, whereas in intersynaptic regions of the axon, vesicle populations were elevated. On the basis of the morphological defects at neuromuscular junctions, we postulate that RAB-3 may regulate recruitment of vesicles to the active zone or sequestration of vesicles near release sites.

Bibliography

Nonet, M. L., Staunton, J. E., Kilgard, M. P., Fergestad, T., Hartwieg, E., Horvitz, H. R., Jorgensen, E. M., & Meyer, B. J. (1997). Caenorhabditis elegans rab-3Mutant Synapses Exhibit Impaired Function and Are Partially Depleted of Vesicles. The Journal of Neuroscience, 17(21), 8061â8073.

Authors 8

Michael L. Nonet (first)
Jane E. Staunton (additional)
Michael P. Kilgard (additional)
Tim Fergestad (additional)
Erika Hartwieg (additional)
H. Robert Horvitz (additional)
Erik M. Jorgensen (additional)
Barbara J. Meyer (additional)

References 61 Referenced 326

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Dates

Type	When
Created	7 years, 4 months ago (April 2, 2018, 8:21 p.m.)
Deposited	2 years, 4 months ago (April 13, 2023, 7:43 a.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:30 p.m.)
Issued	27 years, 9 months ago (Nov. 1, 1997)
Published	27 years, 9 months ago (Nov. 1, 1997)
Published Online	27 years, 9 months ago (Nov. 1, 1997)
Published Print	27 years, 9 months ago (Nov. 1, 1997)

Funders 0

None

BibTeX

@article{Nonet_1997, title={Caenorhabditis elegans rab-3Mutant Synapses Exhibit Impaired Function and Are Partially Depleted of Vesicles}, volume={17}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.17-21-08061.1997}, DOI={10.1523/jneurosci.17-21-08061.1997}, number={21}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Nonet, Michael L. and Staunton, Jane E. and Kilgard, Michael P. and Fergestad, Tim and Hartwieg, Erika and Horvitz, H. Robert and Jorgensen, Erik M. and Meyer, Barbara J.}, year={1997}, month=nov, pages={8061–8073} }

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