DOI: 10.1523/jneurosci.17-03-00960.1997. Multiple Ionic Conductances of the Human Dopamine Transporter: The Actions of Dopamine and Psychostimulants

Multiple Ionic Conductances of the Human Dopamine Transporter: The Actions of Dopamine and Psychostimulants

10.1523/jneurosci.17-03-00960.1997

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

Electrophysiological and pharmacological studies of a cloned human dopamine transporter (hDAT) were undertaken to investigate the mechanisms of transporter function and the actions of drugs at this target. Using two-electrode voltage-clamp techniques with hDAT-expressingXenopus laevisoocytes, we show that hDAT can be considered electrogenic by two criteria. (1) Uptake of hDAT substrates gives rise to a pharmacologically appropriate “transport-associated” current. (2) The velocity of DA uptake measured in oocytes clamped at various membrane potentials was voltage-dependent, increasing with hyperpolarization. Concurrent measurement of transport-associated current and substrate flux in individual oocytes revealed that charge movement during substrate translocation was greater than would be expected for a transport mechanism with fixed stoichiometry of 2 Na+and 1 Cl−per DA+molecule. In addition to the transport-associated current, hDAT also mediates a constitutive leak current, the voltage and ionic dependencies of which differ markedly from those of the transport-associated current. Ion substitution experiments suggest that alkali cations and protons are carried by the hDAT leak conductance. In contrast to the transport-associated functions, the leak does not require Na+or Cl−, and DAT ligands readily interact with the transporter even in the absence of these ions. The currents that hDAT mediates provide a functional assay that readily distinguishes the modes of action of amphetamine-like “DA-releasing” drugs from cocaine-like translocation blockers. In addition, the voltage dependence of DA uptake suggests a mechanism through which presynaptic DA autoreceptor activation may accelerate the termination of dopaminergic neurotransmissionin vivo.

Bibliography

Sonders, M. S., Zhu, S.-J., Zahniser, N. R., Kavanaugh, M. P., & Amara, S. G. (1997). Multiple Ionic Conductances of the Human Dopamine Transporter: The Actions of Dopamine and Psychostimulants. The Journal of Neuroscience, 17(3), 960â974.

Authors 5

Mark S. Sonders (first)
Si-Jia Zhu (additional)
Nancy R. Zahniser (additional)
Michael P. Kavanaugh (additional)
Susan G. Amara (additional)

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Dates

Type	When
Created	7 years, 4 months ago (April 3, 2018, 11 a.m.)
Deposited	1 month, 3 weeks ago (July 3, 2025, 7:23 a.m.)
Indexed	1 month ago (July 25, 2025, 6:03 a.m.)
Issued	28 years, 6 months ago (Feb. 1, 1997)
Published	28 years, 6 months ago (Feb. 1, 1997)
Published Online	28 years, 6 months ago (Feb. 1, 1997)
Published Print	28 years, 6 months ago (Feb. 1, 1997)

Funders 0

None

BibTeX

@article{Sonders_1997, title={Multiple Ionic Conductances of the Human Dopamine Transporter: The Actions of Dopamine and Psychostimulants}, volume={17}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.17-03-00960.1997}, DOI={10.1523/jneurosci.17-03-00960.1997}, number={3}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Sonders, Mark S. and Zhu, Si-Jia and Zahniser, Nancy R. and Kavanaugh, Michael P. and Amara, Susan G.}, year={1997}, month=feb, pages={960–974} }

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