DOI: 10.1523/jneurosci.13-10-04422.1993. Bcl-2 affects survival but not neuronal differentiation of PC12 cells

Bcl-2 affects survival but not neuronal differentiation of PC12 cells

10.1523/jneurosci.13-10-04422.1993

Crossref journal-article

Society for Neuroscience

The Journal of Neuroscience (393)

Abstract

Past studies have shown that serum-free cultures of PC12 cells are a useful model system for studying the mechanisms of neuronal death after neurotrophic factor deprivation. These cultures, as well as NGF- deprived cultures of sympathetic neurons, manifest and endonuclease activity that leads to “apoptotic” internucleosomal DNA cleavage. Overexpression of the proto-oncogene bcl-2 blocks apoptotic death in various cell types. To study the actions of this protein in neuronal cells, we derived PC12 cell lines stably transfected with a cDNA encoding human bcl-2. It is reported here that lines expressing high levels of the exogenous bcl-2 protein are protected from both death and apoptotic DNA fragmentation caused by removal of trophic support. However, expression of high levels of exogenous bcl-2 neither mimics nor interferes with promotion of neurite outgrowth by NGF.

Bibliography

Batistatou, A., Merry, D., Korsmeyer, S., & Greene, L. (1993). Bcl-2 affects survival but not neuronal differentiation of PC12 cells. The Journal of Neuroscience, 13(10), 4422â4428.

Authors 4

A Batistatou (first)
DE Merry (additional)
SJ Korsmeyer (additional)
LA Greene (additional)

References 0 Referenced 100

None

Dates

Type	When
Created	7 years, 5 months ago (March 30, 2018, 8:45 p.m.)
Deposited	2 years, 4 months ago (April 18, 2023, 9:56 a.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 10:32 a.m.)
Issued	31 years, 10 months ago (Oct. 1, 1993)
Published	31 years, 10 months ago (Oct. 1, 1993)
Published Online	31 years, 10 months ago (Oct. 1, 1993)
Published Print	31 years, 10 months ago (Oct. 1, 1993)

Funders 0

None

BibTeX

@article{Batistatou_1993, title={Bcl-2 affects survival but not neuronal differentiation of PC12 cells}, volume={13}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.13-10-04422.1993}, DOI={10.1523/jneurosci.13-10-04422.1993}, number={10}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={Batistatou, A and Merry, DE and Korsmeyer, SJ and Greene, LA}, year={1993}, month=oct, pages={4422–4428} }

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