Crossref journal-article
Society for Neuroscience
The Journal of Neuroscience (393)
Abstract

We have previously shown that retinoic acid-treated cultures of the P19 line of embryonal carcinoma cells differentiate into neurons, glia, and fibroblast-like cells (Jones-Villeneuve et al., 1982). We report here that the monoclonal antibody HNK-1 reacts with the neurons at a very early stage of their differentiation and is, therefore, an early marker of the neuronal lineage. Cells in differentiated P 19 cultures synthesized acetylcholine but not catecholamines, suggesting that at least some of the neurons are cholinergic. The neurons also carry high- affinity uptake sites for GABA but not for serotonin. In long-term cultures, neuronal processes differentiated into axons and dendrites, which formed synapses. This biological system should prove valuable for examining the development and maturation of cholinergic neurons, since their differentiation occurs in cell culture.

Bibliography

McBurney, M., Reuhl, K., Ally, A., Nasipuri, S., Bell, J., & Craig, J. (1988). Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture. The Journal of Neuroscience, 8(3), 1063–1073.

Authors 6
  1. MW McBurney (first)
  2. KR Reuhl (additional)
  3. AI Ally (additional)
  4. S Nasipuri (additional)
  5. JC Bell (additional)
  6. J Craig (additional)
References 0 Referenced 169

None

Dates
Type When
Created 7 years, 5 months ago (March 30, 2018, 5:10 p.m.)
Deposited 2 years, 4 months ago (April 18, 2023, 9:46 a.m.)
Indexed 1 month, 2 weeks ago (July 14, 2025, 11:32 p.m.)
Issued 37 years, 5 months ago (March 1, 1988)
Published 37 years, 5 months ago (March 1, 1988)
Published Online 37 years, 5 months ago (March 1, 1988)
Published Print 37 years, 5 months ago (March 1, 1988)
Funders 0

None

@article{McBurney_1988, title={Differentiation and maturation of embryonal carcinoma-derived neurons in cell culture}, volume={8}, ISSN={1529-2401}, url={http://dx.doi.org/10.1523/jneurosci.08-03-01063.1988}, DOI={10.1523/jneurosci.08-03-01063.1988}, number={3}, journal={The Journal of Neuroscience}, publisher={Society for Neuroscience}, author={McBurney, MW and Reuhl, KR and Ally, AI and Nasipuri, S and Bell, JC and Craig, J}, year={1988}, month=mar, pages={1063–1073} }