DOI: 10.1515/znc-1987-11-1215. Protein Sequence and Structure of N-terminal Amino Acids of Subunit Delta of Spinach Photosynthetic ATP-Synthase CF<sub>1</sub>

Protein Sequence and Structure of N-terminal Amino Acids of Subunit Delta of Spinach Photosynthetic ATP-Synthase CF1

10.1515/znc-1987-11-1215

Crossref journal-article

Walter de Gruyter GmbH

Zeitschrift für Naturforschung C (374)

Abstract

Chloroplast ATP-synthase (CF1) subunit delta (δ) has been isolated from spinach thylakoids in the presence of SDS. By automated Edman degradation and online analysis of PTH derivatives the 35 N-terminal amino acid residues were sequenced. The mature protein starts with: NH2-Val-Asp-Ser-Thr-Ala-Ser-Arg-Tyr-Ala-. This protein sequence allows alignment of spinach δ with the sequences of Z. mays 25 kDa polypeptide, the δ subunit of Rps. blastica, Rsp. rubrum and E. coli F1, and of bovine OSCP, but not with mitochondrial δ. Secondary structure calculations and helical wheel plots reveal a conserved secondary structure. The analyzed N-terminal sequences probably build a short amphipathic alpha helix with two adjacent turns. The such aligned polar residues around Tyr8 of subunit δ are suitable to channel protons.

Bibliography

Berzborn, R. J., Finke, W., Otto, J., & Meyer, H. E. . (1987). Protein Sequence and Structure of N-terminal Amino Acids of Subunit Delta of Spinach Photosynthetic ATP-Synthase CF1. Zeitschrift FÃ¼r Naturforschung C, 42(11â12), 1231â1238.

Authors 4

Richard J. Berzborn (first)
Werner Finke (additional)
Joachim Otto (additional)
Helmut E . Meyer (additional)

References 0 Referenced 11

None

Dates

Type	When
Created	7 years ago (Aug. 2, 2018, 1:49 p.m.)
Deposited	4 years, 2 months ago (June 22, 2021, 5:37 p.m.)
Indexed	2 years, 7 months ago (Jan. 5, 2023, 9:19 p.m.)
Issued	37 years, 8 months ago (Dec. 1, 1987)
Published	37 years, 8 months ago (Dec. 1, 1987)
Published Online	11 years, 2 months ago (June 2, 2014)
Published Print	37 years, 8 months ago (Dec. 1, 1987)

Funders 0

None

BibTeX

@article{Berzborn_1987, title={Protein Sequence and Structure of N-terminal Amino Acids of Subunit Delta of Spinach Photosynthetic ATP-Synthase CF1}, volume={42}, ISSN={0939-5075}, url={http://dx.doi.org/10.1515/znc-1987-11-1215}, DOI={10.1515/znc-1987-11-1215}, number={11–12}, journal={Zeitschrift für Naturforschung C}, publisher={Walter de Gruyter GmbH}, author={Berzborn, Richard J. and Finke, Werner and Otto, Joachim and Meyer, Helmut E .}, year={1987}, month=dec, pages={1231–1238} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        1,
        6
      ]
    ],
    "date-time": "2023-01-06T02:19:25Z",
    "timestamp": 1672971565276
  },
  "reference-count": 0,
  "publisher": "Walter de Gruyter GmbH",
  "issue": "11-12",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1987,
            12,
            1
          ]
        ],
        "date-time": "1987-12-01T00:00:00Z",
        "timestamp": 565315200000
      },
      "content-version": "unspecified",
      "delay-in-days": 0,
      "URL": "http://creativecommons.org/licenses/by-nc-nd/3.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        12,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Chloroplast ATP-synthase (CF<jats:sub>1</jats:sub>) subunit delta (\u03b4) has been isolated from spinach thylakoids in the presence of SDS. By automated Edman degradation and online analysis of PTH derivatives the 35 N-terminal amino acid residues were sequenced. The mature protein starts with: NH<jats:sub>2</jats:sub>-Val-Asp-Ser-Thr-Ala-Ser-Arg-Tyr-Ala-. This protein sequence allows alignment of spinach \u03b4 with the sequences of Z. mays 25 kDa polypeptide, the \u03b4 subunit of Rps. blastica, Rsp. rubrum and E. coli F<jats:sub>1</jats:sub>, and of bovine OSCP, but not with mitochondrial \u03b4.</jats:p>\n               <jats:p>Secondary structure calculations and helical wheel plots reveal a conserved secondary structure. The analyzed N-terminal sequences probably build a short amphipathic alpha helix with two adjacent turns. The such aligned polar residues around Tyr<jats:sub>8</jats:sub> of subunit \u03b4 are suitable to channel protons. </jats:p>",
  "DOI": "10.1515/znc-1987-11-1215",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2018,
        8,
        2
      ]
    ],
    "date-time": "2018-08-02T17:49:42Z",
    "timestamp": 1533232182000
  },
  "page": "1231-1238",
  "source": "Crossref",
  "is-referenced-by-count": 11,
  "title": "Protein Sequence and Structure of N-terminal Amino Acids of Subunit Delta of Spinach Photosynthetic ATP-Synthase CF<sub>1</sub>",
  "prefix": "10.1515",
  "volume": "42",
  "author": [
    {
      "given": "Richard J.",
      "family": "Berzborn",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Lehrstuhl f\u00fcr Biochemie der Pflanzen, Fakult\u00e4t f\u00fcr Biologie, Ruhr-Universit\u00e4t Bochum, Postfach 102148, D -4630 Bochum 1, Bundesrepublik Deutschland"
        }
      ]
    },
    {
      "given": "Werner",
      "family": "Finke",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Lehrstuhl f\u00fcr Biochemie der Pflanzen, Fakult\u00e4t f\u00fcr Biologie, Ruhr-Universit\u00e4t Bochum, Postfach 102148, D -4630 Bochum 1, Bundesrepublik Deutschland"
        }
      ]
    },
    {
      "given": "Joachim",
      "family": "Otto",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Lehrstuhl f\u00fcr Biochemie der Pflanzen, Fakult\u00e4t f\u00fcr Biologie, Ruhr-Universit\u00e4t Bochum, Postfach 102148, D -4630 Bochum 1, Bundesrepublik Deutschland"
        }
      ]
    },
    {
      "given": "Helmut E .",
      "family": "Meyer",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Institut f\u00fcr Physiologische Chemie, Fakult\u00e4t f\u00fcr Medizin, Ruhr-Universit\u00e4t Bochum"
        }
      ]
    }
  ],
  "member": "374",
  "published-online": {
    "date-parts": [
      [
        2014,
        6,
        2
      ]
    ]
  },
  "container-title": "Zeitschrift f\u00fcr Naturforschung C",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://www.degruyter.com/view/j/znc.1987.42.issue-11-12/znc-1987-11-1215/znc-1987-11-1215.xml",
      "content-type": "text/html",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://www.degruyter.com/document/doi/10.1515/znc-1987-11-1215/xml",
      "content-type": "application/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://www.degruyter.com/document/doi/10.1515/znc-1987-11-1215/pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        6,
        22
      ]
    ],
    "date-time": "2021-06-22T21:37:13Z",
    "timestamp": 1624397833000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.degruyter.com/document/doi/10.1515/znc-1987-11-1215/html"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        12,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "11-12",
    "published-online": {
      "date-parts": [
        [
          2014,
          6,
          2
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          1987,
          12,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1515/znc-1987-11-1215"
  ],
  "URL": "http://dx.doi.org/10.1515/znc-1987-11-1215",
  "relation": {},
  "ISSN": [
    "1865-7125",
    "0939-5075"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1987,
        12,
        1
      ]
    ]
  }
}