DOI: 10.1515/znc-1979-3-410. NADPH-Dependent Thioredoxin Reductase and a New Thioredoxin from Wheat

NADPH-Dependent Thioredoxin Reductase and a New Thioredoxin from Wheat

10.1515/znc-1979-3-410

Crossref journal-article

Walter de Gruyter GmbH

Zeitschrift für Naturforschung C (374)

Abstract

Abstract An album in fraction extracted from wheat flour contains thioredoxin reductase (Mr = 65,000) and a heat-stable thioredoxin (Mr = 15,000) which are separated on DEAE cellulose and further purified by gel filtration. W heat thioredoxin stimulates E. coli ribonucleotide reductase but not chloroplast fructose-bis-phosphatase. The enzyme is NADPH -dependent (Km = 3.2 X 10-6 ᴍ) . In presence of the thioredoxin it slowly reduces other proteins like insulin or ribonuclease. Therefore it is most likely identical with a protein disulfide reductase (of unknown specificity) previously described in wheat. This new thioredoxin system is a counterpart of the ferredoxin-dependent system found in photosynthetic plant cells, suggesting different, specific mechanisms for regeneration of reduced thioredoxins in germinating seeds and green plants.

Bibliography

Suske, G., Wagner, W., & Follmann, H. (1979). NADPH-Dependent Thioredoxin Reductase and a New Thioredoxin from Wheat. Zeitschrift FÃ¼r Naturforschung C, 34(3â4), 214â221.

Authors 3

Guntram Suske (first)
Wolfgang Wagner (additional)
Hartmut Follmann (additional)

References 0 Referenced 48

None

Dates

Type	When
Created	7 years, 1 month ago (Aug. 2, 2018, 1:32 p.m.)
Deposited	4 years, 2 months ago (June 22, 2021, 5:28 p.m.)
Indexed	1 year, 10 months ago (Oct. 19, 2023, 8:25 p.m.)
Issued	46 years, 5 months ago (April 1, 1979)
Published	46 years, 5 months ago (April 1, 1979)
Published Online	11 years, 3 months ago (June 2, 2014)
Published Print	46 years, 5 months ago (April 1, 1979)

Funders 0

None

BibTeX

@article{Suske_1979, title={NADPH-Dependent Thioredoxin Reductase and a New Thioredoxin from Wheat}, volume={34}, ISSN={0939-5075}, url={http://dx.doi.org/10.1515/znc-1979-3-410}, DOI={10.1515/znc-1979-3-410}, number={3–4}, journal={Zeitschrift für Naturforschung C}, publisher={Walter de Gruyter GmbH}, author={Suske, Guntram and Wagner, Wolfgang and Follmann, Hartmut}, year={1979}, month=apr, pages={214–221} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        20
      ]
    ],
    "date-time": "2023-10-20T00:25:12Z",
    "timestamp": 1697761512670
  },
  "reference-count": 0,
  "publisher": "Walter de Gruyter GmbH",
  "issue": "3-4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1979,
            4,
            1
          ]
        ],
        "date-time": "1979-04-01T00:00:00Z",
        "timestamp": 291772800000
      },
      "content-version": "unspecified",
      "delay-in-days": 0,
      "URL": "http://creativecommons.org/licenses/by-nc-nd/3.0/"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1979,
        4,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p> An album in fraction extracted from wheat flour contains thioredoxin reductase (Mr = 65,000) and a heat-stable thioredoxin (M<jats:sub>r</jats:sub> = 15,000) which are separated on DEAE cellulose and further purified by gel filtration. W heat thioredoxin stimulates E. coli ribonucleotide reductase but not chloroplast fructose-bis-phosphatase. The enzyme is NADPH -dependent (K<jats:sub>m</jats:sub> = 3.2 X 10<jats:sup>-6</jats:sup> \u1d0d) . In presence of the thioredoxin it slowly reduces other proteins like insulin or ribonuclease. Therefore it is most likely identical with a protein disulfide reductase (of unknown specificity) previously described in wheat. This new thioredoxin system is a counterpart of the ferredoxin-dependent system found in photosynthetic plant cells, suggesting different, specific mechanisms for regeneration of reduced thioredoxins in germinating seeds and green plants.</jats:p>",
  "DOI": "10.1515/znc-1979-3-410",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2018,
        8,
        2
      ]
    ],
    "date-time": "2018-08-02T17:32:07Z",
    "timestamp": 1533231127000
  },
  "page": "214-221",
  "source": "Crossref",
  "is-referenced-by-count": 48,
  "title": "NADPH-Dependent Thioredoxin Reductase and a New Thioredoxin from Wheat",
  "prefix": "10.1515",
  "volume": "34",
  "author": [
    {
      "given": "Guntram",
      "family": "Suske",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Fachbereich Chemie (Biochemie) der Philipps-Universit\u00e4t Marburg, Lahnberge, D-3550 Marburg"
        }
      ]
    },
    {
      "given": "Wolfgang",
      "family": "Wagner",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Fachbereich Chemie (Biochemie) der Philipps-Universit\u00e4t Marburg, Lahnberge, D-3550 Marburg"
        }
      ]
    },
    {
      "given": "Hartmut",
      "family": "Follmann",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Fachbereich Chemie (Biochemie) der Philipps-Universit\u00e4t Marburg, Lahnberge, D-3550 Marburg"
        }
      ]
    }
  ],
  "member": "374",
  "published-online": {
    "date-parts": [
      [
        2014,
        6,
        2
      ]
    ]
  },
  "container-title": "Zeitschrift f\u00fcr Naturforschung C",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.degruyter.com/view/journals/znc/34/3-4/article-p214.xml",
      "content-type": "text/html",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://www.degruyter.com/document/doi/10.1515/znc-1979-3-410/xml",
      "content-type": "application/xml",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://www.degruyter.com/document/doi/10.1515/znc-1979-3-410/pdf",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        6,
        22
      ]
    ],
    "date-time": "2021-06-22T21:28:00Z",
    "timestamp": 1624397280000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.degruyter.com/document/doi/10.1515/znc-1979-3-410/html"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1979,
        4,
        1
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "3-4",
    "published-online": {
      "date-parts": [
        [
          2014,
          6,
          2
        ]
      ]
    },
    "published-print": {
      "date-parts": [
        [
          1979,
          4,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1515/znc-1979-3-410"
  ],
  "URL": "http://dx.doi.org/10.1515/znc-1979-3-410",
  "relation": {},
  "ISSN": [
    "1865-7125",
    "0939-5075"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1979,
        4,
        1
      ]
    ]
  }
}