DOI: 10.1515/znc-1976-7-819. On the Reconstitution of Photophosphorylation in CF<sub>1</sub>-Extracted Chloroplasts

On the Reconstitution of Photophosphorylation in CF1-Extracted Chloroplasts

10.1515/znc-1976-7-819

Crossref journal-article

Walter de Gruyter GmbH

Zeitschrift für Naturforschung C (374)

Abstract

Abstract 1. A method for the reproducible preparation of CF1-depleted thylakoid membranes was established. By repeated washes with pyrophosphate solution and subsequent treatment with sucrose media, containing micromolar concentrations of Ca2+ or Mg2+, different degrees of CF1 depletion and uncoupling were adjusted. 2. The membranes were completely uncoupled when about 50% CF1 was removed. Re-coupling with isolated CF1 was maximal at this point. It decreased with progressive CF1 extraction. 3. The re-attached CF1 particles regained their ability of energy-dependent adenine nucleotide exchange, an essential reaction in photophosphorylation. 4. CF1-depleted membranes showed two main morphological alterations, a. unstacknig of the grana, and b. a perforation of the thylakoid membranes in those chloroplasts which showed a poor recoupling activity. Whereas CF1-depletion and unstacking were found to be reversible, the formation of holes was an irreversible effect. 5. The decreasing re-coupling activity was discussed in context to the irreversible perforation of the thylakoid membranes.

Bibliography

Hesse, H., Jank-Ladwig, R., & Strotmann, H. (1976). On the Reconstitution of Photophosphorylation in CF1-Extracted Chloroplasts. Zeitschrift FÃ¼r Naturforschung C, 31(7â8), 445â451.

Authors 3

H Hesse (first)
Rita Jank-Ladwig (additional)
H Strotmann (additional)

References 0 Referenced 26

None

Dates

Type	When
Created	7 years ago (Aug. 2, 2018, 1:26 p.m.)
Deposited	4 years, 2 months ago (June 22, 2021, 9:56 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 9:59 a.m.)
Issued	49 years ago (Aug. 1, 1976)
Published	49 years ago (Aug. 1, 1976)
Published Online	11 years, 2 months ago (June 2, 2014)
Published Print	49 years ago (Aug. 1, 1976)

Funders 0

None

BibTeX

@article{Hesse_1976, title={On the Reconstitution of Photophosphorylation in CF1-Extracted Chloroplasts}, volume={31}, ISSN={0939-5075}, url={http://dx.doi.org/10.1515/znc-1976-7-819}, DOI={10.1515/znc-1976-7-819}, number={7–8}, journal={Zeitschrift für Naturforschung C}, publisher={Walter de Gruyter GmbH}, author={Hesse, H and Jank-Ladwig, Rita and Strotmann, H}, year={1976}, month=aug, pages={445–451} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p> 1. A method for the reproducible preparation of CF<jats:sub>1</jats:sub>-depleted thylakoid membranes was established. By repeated washes with pyrophosphate solution and subsequent treatment with sucrose media, containing micromolar concentrations of Ca<jats:sup>2+</jats:sup> or Mg<jats:sup>2+</jats:sup>, different degrees of CF<jats:sub>1</jats:sub> depletion and uncoupling were adjusted.</jats:p>\n               <jats:p>2. The membranes were completely uncoupled when about 50% CF<jats:sub>1</jats:sub> was removed. Re-coupling with isolated CF<jats:sub>1</jats:sub> was maximal at this point. It decreased with progressive CF<jats:sub>1</jats:sub> extraction.</jats:p>\n               <jats:p>3. The re-attached CF<jats:sub>1</jats:sub> particles regained their ability of energy-dependent adenine nucleotide exchange, an essential reaction in photophosphorylation.</jats:p>\n               <jats:p>4. CF<jats:sub>1</jats:sub>-depleted membranes showed two main morphological alterations,</jats:p>\n               <jats:p>a. unstacknig of the grana, and</jats:p>\n               <jats:p>b. a perforation of the thylakoid membranes in those chloroplasts which showed a poor recoupling activity. Whereas CF<jats:sub>1</jats:sub>-depletion and unstacking were found to be reversible, the formation of holes was an irreversible effect.</jats:p>\n               <jats:p>5. The decreasing re-coupling activity was discussed in context to the irreversible perforation of the thylakoid membranes.</jats:p>",
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