Abstract
An improved method for the electron microscopic study of sectioned tissue cultures of human brain tumors is based on commercial perforated cellophane sheets upon which the cells are grown. Specimens are fixed with buffered osmium tetroxide, imbedded horizontally in Metacrylate or Vestopal without separating them from the cellophane sheet and the monolayer cut in sections of 500 A. Electron micrographs show a very good preservation of intracellular and intercellular structures.
Dates
Type | When |
---|---|
Created | 10 years, 5 months ago (March 3, 2015, 12:43 p.m.) |
Deposited | 4 years, 2 months ago (June 23, 2021, 7:45 a.m.) |
Indexed | 3 years, 4 months ago (March 30, 2022, 1:56 p.m.) |
Issued | 60 years, 3 months ago (May 1, 1965) |
Published | 60 years, 3 months ago (May 1, 1965) |
Published Online | 11 years, 2 months ago (June 2, 2014) |
Published Print | 60 years, 3 months ago (May 1, 1965) |
@article{Zang_1965, title={Ein Verfahren zur elektronenmikroskopischen Untersuchung von Gewebekulturen im Wuchsverband}, volume={20}, ISSN={0932-0776}, url={http://dx.doi.org/10.1515/znb-1965-0513}, DOI={10.1515/znb-1965-0513}, number={5}, journal={Zeitschrift für Naturforschung B}, publisher={Walter de Gruyter GmbH}, author={Zang, K. D. and Luh, S. and Hager, H.}, year={1965}, month=may, pages={477–476b} }