DOI: 10.1515/bc.2000.122. Helical Tubes of FtsZ from Methanococcus jannaschii

Helical Tubes of FtsZ from Methanococcus jannaschii

10.1515/bc.2000.122

Crossref journal-article

Walter de Gruyter GmbH

Biological Chemistry (374)

Abstract

AbstractBacterial cell division depends on the formation of a cytokinetic ring structure, the Z-ring. The bacterial tubulin homologue FtsZ is required for Z-ring formation. FtsZ assembles into various polymeric formsin vitro, indicating a structural role in the septum of bacteria. We have used recombinant FtsZ1 protein fromM. jannaschiito produce helical tubes and sheets with high yield using the GTP analogue GMPCPP [guanylyl-(α,β)-methylene-diphosphate]. The sheets appear identical to the previously reported Ca++-induced sheets of FtsZ fromM. jannaschiithat were shown to consist of ‘thick’-filaments in which two protofilaments run in parallel. Tubes assembled either in Ca++or in GMPCPP contain filaments whose dimensions indicate that they could be equivalent to the ‘thick’-filaments in sheets. Some tubes are hollow but others are filled by additional protein density. Helical FtsZ tubes differ from eukaryotic microtubules in that the filaments curve around the filament axis with a pitch of ~ 430 Å for Ca++-induced tubes or 590–620 Å for GMPCPP. However, their assemblyin vitroas well-ordered polymers over distances comparable to the inner circumference of a bacterium may indicate a rolein vivo. Their size and stability make them suitable for use in motility assays.

Bibliography

LÃ¶we, J., & Amos, L. A. (2000). Helical Tubes of FtsZ from Methanococcus jannaschii. Biological Chemistry, 381(9â10), 993â999.

Authors 2

Jan Löwe (first)
Linda A. Amos (additional)

References 35 Referenced 37

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Dates

Type	When
Created	20 years, 10 months ago (Oct. 11, 2004, 12:25 p.m.)
Deposited	4 years, 4 months ago (April 21, 2021, 5:14 p.m.)
Indexed	1 year, 5 months ago (March 11, 2024, 7:05 p.m.)
Issued	24 years, 11 months ago (Sept. 13, 2000)
Published	24 years, 11 months ago (Sept. 13, 2000)
Published Print	24 years, 11 months ago (Sept. 13, 2000)

Funders 0

None

BibTeX

@article{L_we_2000, title={Helical Tubes of FtsZ from Methanococcus jannaschii}, volume={381}, ISSN={1431-6730}, url={http://dx.doi.org/10.1515/bc.2000.122}, DOI={10.1515/bc.2000.122}, number={9–10}, journal={Biological Chemistry}, publisher={Walter de Gruyter GmbH}, author={Löwe, Jan and Amos, Linda A.}, year={2000}, month=sep, pages={993–999} }

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