DOI: 10.1371/journal.ppat.1002758. Identification of a General O-linked Protein Glycosylation System in Acinetobacter baumannii and Its Role in Virulence and Biofilm Formation

Identification of a General O-linked Protein Glycosylation System in Acinetobacter baumannii and Its Role in Virulence and Biofilm Formation

10.1371/journal.ppat.1002758

Crossref journal-article

Public Library of Science (PLoS)

PLoS Pathogens (340)

Bibliography

Iwashkiw, J. A., Seper, A., Weber, B. S., Scott, N. E., Vinogradov, E., Stratilo, C., Reiz, B., Cordwell, S. J., Whittal, R., Schild, S., & Feldman, M. F. (2012). Identification of a General O-linked Protein Glycosylation System in Acinetobacter baumannii and Its Role in Virulence and Biofilm Formation. PLoS Pathogens, 8(6), e1002758.

Authors 11

Jeremy A. Iwashkiw (first)
Andrea Seper (additional)
Brent S. Weber (additional)
Nichollas E. Scott (additional)
Evgeny Vinogradov (additional)
Chad Stratilo (additional)
Bela Reiz (additional)
Stuart J. Cordwell (additional)
Randy Whittal (additional)
Stefan Schild (additional)
Mario F. Feldman (additional)

References 71 Referenced 194

10.1016/j.jhin.2009.03.032 / J Hosp Infect / <italic>Acinetobacter</italic>: an old friend, but a new enemy. by KJ Towner (2009)
10.1128/CMR.00058-07 / Clin Microbiol Rev / <italic>Acinetobacter baumannii</italic>: emergence of a successful pathogen. by AY Peleg (2008)
10.1007/s00134-003-1661-y / Intensive Care Med / <italic>Acinetobacter baumannii</italic>: a threat for the ICU? by J Rello (2003)
10.1111/j.1472-765X.2005.01791.x / Lett Appl Microbiol / The emergence of multidrug resistant <italic>Acinetobacter</italic> species: a major concern in the hospital setting. by GW Hanlon (2005)
10.1101/gad.1510307 / Genes Dev / New insights into <italic>Acinetobacter baumannii</italic> pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis. by MG Smith (2007)
10.1086/500202 / Clin Infect Dis / The epidemiology and control of <italic>Acinetobacter baumannii</italic> in health care facilities. by PE Fournier (2006)
10.1016/j.ijantimicag.2008.02.013 / Int J Antimicrob Agents / <italic>Acinetobacter baumannii</italic>: a universal threat to public health? by H Giamarellou (2008)
10.1086/652759 / Clin Infect Dis / Multidrug-resistant <italic>Acinetobacter baumannii</italic>: an emerging pathogen among older adults in community hospitals and nursing homes. by DM Sengstock (2010)
10.1128/AAC.01097-08 / Antimicrob Agents Chemother / In vitro activities of tigecycline, minocycline, and colistin-tigecycline combination against multi- and pandrug-resistant clinical isolates of <italic>Acinetobacter baumannii</italic> group. by LA Arroyo (2009)
{'key': 'ref10', 'first-page': '309', 'article-title': '<italic>Acinetobacter baumannii</italic> outer membrane protein A targets the nucleus and induces cytotoxicity.', 'volume': '10', 'author': 'CH Choi', 'year': '2008', 'journal-title': 'Cell Microbiol'} / Cell Microbiol / <italic>Acinetobacter baumannii</italic> outer membrane protein A targets the nucleus and induces cytotoxicity. by CH Choi (2008)
10.1099/mic.0.2008/019471-0 / Microbiology / Characterization of a two-component regulatory system from <italic>Acinetobacter baumannii</italic> that controls biofilm formation and cellular morphology. by AP Tomaras (2008)
10.1128/JB.00647-09 / J Bacteriol / The <italic>pga</italic>ABCD locus of <italic>Acinetobacter baumannii</italic> encodes the production of poly-beta-1-6-N-acetylglucosamine, which is critical for biofilm formation. by AH Choi (2009)
10.1016/j.ijantimicag.2009.10.024 / Int J Antimicrob Agents / Multidrug-resistant <italic>Acinetobacter baumannii</italic>: mechanisms of virulence and resistance. by NC Gordon (2010)
10.1016/S0944-5013(01)80004-5 / Microbiol Res / Detection and characterization of quorum sensing signal molecules in <italic>Acinetobacter</italic> strains. by RH Gonzalez (2001)
10.1038/nrmicro2383 / Nat Rev Microbiol / Protein glycosylation in bacteria: sweeter than ever. by H Nothaft (2010)
10.1073/pnas.0809504106 / Proc Natl Acad Sci U S A / Broad spectrum <italic>O</italic>-linked protein glycosylation in the human pathogen <italic>Neisseria gonorrhoeae</italic>. by A Vik (2009)
10.1016/j.cell.2009.02.041 / Cell / A general <italic>O</italic>-glycosylation system important to the physiology of a major human intestinal symbiont. by CM Fletcher (2009)
10.1038/sj.emboj.7601024 / Embo J / Two distinct but interchangeable mechanisms for flipping of lipid-linked oligosaccharides. by C Alaimo (2006)
10.1128/JB.01318-07 / J Bacteriol / Functional characterization of bacterial oligosaccharyltransferases involved in <italic>O</italic>-linked protein glycosylation. by A Faridmoayer (2007)
10.1074/mcp.M000031-MCP201 / Mol Cell Proteomics / Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the <italic>N</italic>-linked glycoproteome of <italic>Campylobacter jejuni</italic>. by NE Scott (2011)
10.1016/j.bbrc.2006.06.182 / Biochem Biophys Res Commun / Pilin glycosylation in <italic>Neisseria meningitidis</italic> occurs by a similar pathway to wzy-dependent <italic>O</italic>-antigen biosynthesis in <italic>Escherichia coli</italic>. by PM Power (2006)
{'key': 'ref22', 'article-title': 'Characterization of exogenous bacterial oligosaccharyltransferases in <italic>Escherichia coli</italic> reveals the potential for <italic>O</italic>-linked protein glycosylation in <italic>Vibrio cholerae</italic> and <italic>Burkholderia thailandensis</italic>. Glycobiology.', 'author': 'C Gebhart', 'year': '2012'} / Characterization of exogenous bacterial oligosaccharyltransferases in <italic>Escherichia coli</italic> reveals the potential for <italic>O</italic>-linked protein glycosylation in <italic>Vibrio cholerae</italic> and <italic>Burkholderia thailandensis</italic>. Glycobiology. by C Gebhart (2012)
10.1093/glycob/cwq148 / Glycobiology / Analogies and homologies in lipopolysaccharide and glycoprotein biosynthesis in bacteria. by I Hug (2011)
10.1093/nar/gkp919 / Nucleic Acids Res / MicrobesOnline: an integrated portal for comparative and functional genomics. by PS Dehal (2010)
10.1128/JCM.36.5.1245-1250.1998 / J Clin Microbiol / Specificity of rabbit antisera against lipopolysaccharide of <italic>Acinetobacter</italic>. by R Pantophlet (1998)
10.1021/pr101299j / J Proteome Res / Proteomic and Functional Analyses Reveal a Unique Lifestyle for <italic>Acinetobacter baumannii</italic> Biofilms and a Key Role for Histidine Metabolism. by MP Cabral (2011)
10.1002/pmic.200800780 / Proteomics / First evidence of a membrane-bound, tyramine and beta-phenylethylamine producing, tyrosine decarboxylase in <italic>Enterococcus faecalis</italic>: a two-dimensional electrophoresis proteomic study. by E Pessione (2009)
10.2217/fmb.09.5 / Future Microbiol / Regulation of <italic>Acinetobacter baumannii</italic> biofilm formation. by JA Gaddy (2009)
10.1128/IAI.06279-11 / Infect Immun / Role of Acinetobactin-Mediated Iron Acquisition Functions in the Interaction of <italic>Acinetobacter baumannii</italic> Strain ATCC 19606T with Human Lung Epithelial Cells, <italic>Galleria mellonella</italic> Caterpillars, and Mice. by JA Gaddy (2012)
10.1099/jmm.0.037523-0 / J Med Microbiol / Virulence of strains of <italic>Acinetobacter baumannii</italic> is enhanced in <italic>Galleria mellonella</italic> following biofilm adaptation. by M Wand (2011)
10.1371/journal.pone.0022674 / PLoS One / Deciphering the multifactorial nature of <italic>Acinetobacter baumannii</italic> pathogenicity. by LC Antunes (2011)
10.1128/AAC.01533-08 / Antimicrob Agents Chemother / <italic>Galleria mellonella</italic> as a model system to study <italic>Acinetobacter baumannii</italic> pathogenesis and therapeutics. by AY Peleg (2009)
10.1128/AAC.00230-11 / Antimicrob Agents Chemother / <italic>In vivo</italic> efficacy of glycopeptide-colistin combination therapies in a <italic>Galleria mellonella</italic> model of <italic>Acinetobacter baumannii</italic> infection. by M Hornsey (2011)
10.1128/IAI.01233-10 / Infect Immun / <italic>Dictyostelium discoideum</italic> as a model system for identification of <italic>Burkholderia pseudomallei</italic> virulence factors. by BM Hasselbring (2011)
10.1128/JB.182.13.3843-3845.2000 / J Bacteriol / Positive correlation between virulence of <italic>Pseudomonas aeruginosa</italic> mutants in mice and insects. by G Jander (2000)
10.1128/IAI.73.7.3842-3850.2005 / Infect Immun / <italic>Galleria mellonella</italic> as a model system to study <italic>Cryptococcus neoformans</italic> pathogenesis. by E Mylonakis (2005)
10.1093/infdis/jiq086 / J Infect Dis / Impaired virulence and <italic>in vivo</italic> fitness of colistin-resistant <italic>Acinetobacter baumannii</italic>. by R Lopez-Rojas (2010)
10.1111/j.1469-0691.2011.03695.x / Clin Microbiol Infect / <italic>In vitro</italic> and <italic>in vivo</italic> reduced fitness and virulence in ciprofloxacin-resistant <italic>Acinetobacter baumannii</italic>. by Y Smani (2011)
10.1128/IAI.72.7.4275-4278.2004 / Infect Immun / <italic>Pseudomonas aeruginosa</italic> pyocyanin is critical for lung infection in mice. by GW Lau (2004)
10.1073/pnas.1104404108 / Proc Natl Acad Sci U S A / Genome-wide recombination drives diversification of epidemic strains of <italic>Acinetobacter baumannii</italic>. by ES Snitkin (2011)
10.1128/IAI.70.4.2242-2244.2002 / Infect Immun / <italic>Campylobacter</italic> protein glycosylation affects host cell interactions. by CM Szymanski (2002)
10.1128/JB.00101-10 / J Bacteriol / Genetic, structural, and antigenic analyses of glycan diversity in the <italic>O</italic>-linked protein glycosylation systems of human <italic>Neisseria</italic> species. by B Borud (2011)
10.1111/j.1462-5822.2011.01586.x / Cell Microbiol / <italic>Neisseria gonorrhoeae</italic> pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells. by MP Jennings (2011)
10.1074/jbc.M110.194506 / J Biol Chem / Theoretical and experimental characterization of the scope of protein <italic>O</italic>-glycosylation in <italic>Bacteroides fragilis</italic>. by CM Fletcher (2011)
{'key': 'ref45', 'first-page': '1784', 'article-title': '[Structure of lipopolysaccharides from gram-negative bacteria. III. Structure of O-specific polysaccharides].', 'volume': '59', 'author': 'YA Knirel', 'year': '1994', 'journal-title': 'Biokhimiia'} / Biokhimiia / [Structure of lipopolysaccharides from gram-negative bacteria. III. Structure of O-specific polysaccharides]. by YA Knirel (1994)
10.1021/bi052227i / Biochemistry / Peptidoglycan recognition by Pal, an outer membrane lipoprotein. by LM Parsons (2006)
10.1016/j.jtbi.2007.10.039 / J Theor Biol / Microcolony and biofilm formation as a survival strategy for bacteria. by LR Johnson (2008)
10.1007/978-94-007-0940-9_13 / Adv Exp Med Biol / Bacterial extracellular polysaccharides. by K Bazaka (2011)
10.4248/IJOS11026 / Int J Oral Sci / The clinical impact of bacterial biofilms. by N Hoiby (2011)
10.1128/jb.184.4.1140-1154.2002 / J Bacteriol / <italic>Pseudomonas aeruginosa</italic> displays multiple phenotypes during development as a biofilm. by K Sauer (2002)
10.1046/j.1365-2958.1998.01062.x / Mol Microbiol / Flagellar and twitching motility are necessary for <italic>Pseudomonas aeruginosa</italic> biofilm development. by GA O'Toole (1998)
10.1016/j.mib.2008.05.005 / Curr Opin Microbiol / Eat, kill or die: when amoeba meets bacteria. by P Cosson (2008)
10.1128/IAI.64.2.668-673.1996 / Infect Immun / Interaction of <italic>Legionella pneumophila</italic> with <italic>Acanthamoeba castellanii</italic>: uptake by coiling phagocytosis and inhibition of phagosome-lysosome fusion. by JA Bozue (1996)
10.1016/j.femsre.2003.09.002 / FEMS Microbiol Rev / Exploiting the potential of insects for <italic>in vivo</italic> pathogenicity testing of microbial pathogens. by K Kavanagh (2004)
10.1128/IAI.68.10.5716-5723.2000 / Infect Immun / Identification and characterization of the <italic>Brucella abortus</italic> phosphoglucomutase gene: role of lipopolysaccharide in virulence and intracellular multiplication. by JE Ugalde (2000)
10.1016/j.vetmic.2007.06.007 / Vet Microbiol / Glycosylation of type-IV fimbriae of <italic>Dichelobacter nodosus</italic>. by TI Cagatay (2008)
{'key': 'ref57', 'first-page': '237', 'article-title': 'Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.', 'volume': '347', 'author': 'CL Marolda', 'year': '2006', 'journal-title': 'Methods Mol Biol'} / Methods Mol Biol / Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide. by CL Marolda (2006)
10.1002/pmic.200900570 / Proteomics / The effect of interfering ions on search algorithm performance for electron-transfer dissociation data. by DM Good (2010)
10.1016/j.jasms.2009.03.006 / J Am Soc Mass Spectrom / Post-acquisition ETD spectral processing for increased peptide identifications. by DM Good (2009)
10.1111/j.1365-2958.2011.07867.x / Mol Microbiol / Extracellular nucleases and extracellular DNA play important roles in <italic>Vibrio cholerae</italic> biofilm formation. by A Seper (2011)
10.1099/00221287-146-10-2395 / Microbiology 146 ( Pt / Quantification of biofilm structures by the novel computer program COMSTAT. by A Heydorn (2000)
10.1073/pnas.052704399 / Proc Natl Acad Sci U S A / The human pathogen <italic>Pseudomonas aeruginosa</italic> utilizes conserved virulence pathways to infect the social amoeba <italic>Dictyostelium discoideum</italic>. by S Pukatzki (2002)
10.1093/jac/dkh080 / J Antimicrob Chemother / <italic>In vitro</italic> and <italic>in vivo</italic> activity of meropenem and sulbactam against a multidrug-resistant <italic>Acinetobacter baumannii</italic> strain. by WC Ko (2004)
10.1016/j.micpath.2011.09.004 / Microb Pathog / Protection against <italic>Acinetobacter baumannii</italic> infection via its functional deprivation of biofilm associated protein (Bap). by Y Fattahian (2011)
10.1128/IAI.00741-10 / Infect Immun / Vaccination with outer membrane complexes elicits rapid protective immunity to multidrug-resistant <italic>Acinetobacter baumannii</italic>. by MJ McConnell (2011)
10.1084/jem.91.2.231 / J Exp Med / The virulence and immunogenicity of strains derived from <italic>Salmonella typhosa</italic> 58 (Panama carrier). by HC Batson (1950)
10.1016/j.vaccine.2010.10.052 / Vaccine / Active and passive immunization against <italic>Acinetobacter baumannii</italic> using an inactivated whole cell vaccine. by MJ McConnell (2010)
{'key': 'ref68', 'first-page': '97', 'article-title': '[Studies of 26 strains of Moraxella Iwoffi].', 'volume': '80', 'author': 'M Piechaud', 'year': '1951', 'journal-title': 'Ann Inst Pasteur (Paris)'} / Ann Inst Pasteur (Paris) / [Studies of 26 strains of Moraxella Iwoffi]. by M Piechaud (1951)
10.1016/0378-1119(96)00289-2 / Gene / A set of compatible tac promoter expression vectors. by DM Dykxhoorn (1996)
10.1016/0378-1119(90)90494-C / Gene / Analysis and nucleotide sequence of an origin of DNA replication in <italic>Acinetobacter calcoaceticus</italic> and its use for <italic>Escherichia coli</italic> shuttle plasmids. by M Hunger (1990)
10.1016/S0378-1119(98)00130-9 / Gene / A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked <italic>Pseudomonas aeruginosa</italic> mutants. by TT Hoang (1998)

Dates

Type	When
Created	13 years, 2 months ago (June 7, 2012, 5:23 p.m.)
Deposited	5 years, 1 month ago (July 8, 2020, 12:55 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 8:42 a.m.)
Issued	13 years, 2 months ago (June 7, 2012)
Published	13 years, 2 months ago (June 7, 2012)
Published Online	13 years, 2 months ago (June 7, 2012)

Funders 0

None

BibTeX

@article{Iwashkiw_2012, title={Identification of a General O-linked Protein Glycosylation System in Acinetobacter baumannii and Its Role in Virulence and Biofilm Formation}, volume={8}, ISSN={1553-7374}, url={http://dx.doi.org/10.1371/journal.ppat.1002758}, DOI={10.1371/journal.ppat.1002758}, number={6}, journal={PLoS Pathogens}, publisher={Public Library of Science (PLoS)}, author={Iwashkiw, Jeremy A. and Seper, Andrea and Weber, Brent S. and Scott, Nichollas E. and Vinogradov, Evgeny and Stratilo, Chad and Reiz, Bela and Cordwell, Stuart J. and Whittal, Randy and Schild, Stefan and Feldman, Mario F.}, editor={Isberg, Ralph R.}, year={2012}, month=jun, pages={e1002758} }

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      "first-page": "355",
      "DOI": "10.1016/j.jhin.2009.03.032",
      "article-title": "<italic>Acinetobacter</italic>: an old friend, but a new enemy.",
      "volume": "73",
      "author": "KJ Towner",
      "year": "2009",
      "journal-title": "J Hosp Infect"
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    {
      "key": "ref2",
      "doi-asserted-by": "crossref",
      "first-page": "538",
      "DOI": "10.1128/CMR.00058-07",
      "article-title": "<italic>Acinetobacter baumannii</italic>: emergence of a successful pathogen.",
      "volume": "21",
      "author": "AY Peleg",
      "year": "2008",
      "journal-title": "Clin Microbiol Rev"
    },
    {
      "key": "ref3",
      "doi-asserted-by": "crossref",
      "first-page": "350",
      "DOI": "10.1007/s00134-003-1661-y",
      "article-title": "<italic>Acinetobacter baumannii</italic>: a threat for the ICU?",
      "volume": "29",
      "author": "J Rello",
      "year": "2003",
      "journal-title": "Intensive Care Med"
    },
    {
      "key": "ref4",
      "doi-asserted-by": "crossref",
      "first-page": "375",
      "DOI": "10.1111/j.1472-765X.2005.01791.x",
      "article-title": "The emergence of multidrug resistant <italic>Acinetobacter</italic> species: a major concern in the hospital setting.",
      "volume": "41",
      "author": "GW Hanlon",
      "year": "2005",
      "journal-title": "Lett Appl Microbiol"
    },
    {
      "key": "ref5",
      "doi-asserted-by": "crossref",
      "first-page": "601",
      "DOI": "10.1101/gad.1510307",
      "article-title": "New insights into <italic>Acinetobacter baumannii</italic> pathogenesis revealed by high-density pyrosequencing and transposon mutagenesis.",
      "volume": "21",
      "author": "MG Smith",
      "year": "2007",
      "journal-title": "Genes Dev"
    },
    {
      "key": "ref6",
      "doi-asserted-by": "crossref",
      "first-page": "692",
      "DOI": "10.1086/500202",
      "article-title": "The epidemiology and control of <italic>Acinetobacter baumannii</italic> in health care facilities.",
      "volume": "42",
      "author": "PE Fournier",
      "year": "2006",
      "journal-title": "Clin Infect Dis"
    },
    {
      "key": "ref7",
      "doi-asserted-by": "crossref",
      "first-page": "106",
      "DOI": "10.1016/j.ijantimicag.2008.02.013",
      "article-title": "<italic>Acinetobacter baumannii</italic>: a universal threat to public health?",
      "volume": "32",
      "author": "H Giamarellou",
      "year": "2008",
      "journal-title": "Int J Antimicrob Agents"
    },
    {
      "key": "ref8",
      "doi-asserted-by": "crossref",
      "first-page": "1611",
      "DOI": "10.1086/652759",
      "article-title": "Multidrug-resistant <italic>Acinetobacter baumannii</italic>: an emerging pathogen among older adults in community hospitals and nursing homes.",
      "volume": "50",
      "author": "DM Sengstock",
      "year": "2010",
      "journal-title": "Clin Infect Dis"
    },
    {
      "key": "ref9",
      "doi-asserted-by": "crossref",
      "first-page": "1295",
      "DOI": "10.1128/AAC.01097-08",
      "article-title": "In vitro activities of tigecycline, minocycline, and colistin-tigecycline combination against multi- and pandrug-resistant clinical isolates of <italic>Acinetobacter baumannii</italic> group.",
      "volume": "53",
      "author": "LA Arroyo",
      "year": "2009",
      "journal-title": "Antimicrob Agents Chemother"
    },
    {
      "key": "ref10",
      "first-page": "309",
      "article-title": "<italic>Acinetobacter baumannii</italic> outer membrane protein A targets the nucleus and induces cytotoxicity.",
      "volume": "10",
      "author": "CH Choi",
      "year": "2008",
      "journal-title": "Cell Microbiol"
    },
    {
      "key": "ref11",
      "doi-asserted-by": "crossref",
      "first-page": "3398",
      "DOI": "10.1099/mic.0.2008/019471-0",
      "article-title": "Characterization of a two-component regulatory system from <italic>Acinetobacter baumannii</italic> that controls biofilm formation and cellular morphology.",
      "volume": "154",
      "author": "AP Tomaras",
      "year": "2008",
      "journal-title": "Microbiology"
    },
    {
      "key": "ref12",
      "doi-asserted-by": "crossref",
      "first-page": "5953",
      "DOI": "10.1128/JB.00647-09",
      "article-title": "The <italic>pga</italic>ABCD locus of <italic>Acinetobacter baumannii</italic> encodes the production of poly-beta-1-6-N-acetylglucosamine, which is critical for biofilm formation.",
      "volume": "191",
      "author": "AH Choi",
      "year": "2009",
      "journal-title": "J Bacteriol"
    },
    {
      "key": "ref13",
      "doi-asserted-by": "crossref",
      "first-page": "219",
      "DOI": "10.1016/j.ijantimicag.2009.10.024",
      "article-title": "Multidrug-resistant <italic>Acinetobacter baumannii</italic>: mechanisms of virulence and resistance.",
      "volume": "35",
      "author": "NC Gordon",
      "year": "2010",
      "journal-title": "Int J Antimicrob Agents"
    },
    {
      "key": "ref14",
      "doi-asserted-by": "crossref",
      "first-page": "271",
      "DOI": "10.1016/S0944-5013(01)80004-5",
      "article-title": "Detection and characterization of quorum sensing signal molecules in <italic>Acinetobacter</italic> strains.",
      "volume": "155",
      "author": "RH Gonzalez",
      "year": "2001",
      "journal-title": "Microbiol Res"
    },
    {
      "key": "ref15",
      "doi-asserted-by": "crossref",
      "first-page": "765",
      "DOI": "10.1038/nrmicro2383",
      "article-title": "Protein glycosylation in bacteria: sweeter than ever.",
      "volume": "8",
      "author": "H Nothaft",
      "year": "2010",
      "journal-title": "Nat Rev Microbiol"
    },
    {
      "key": "ref16",
      "doi-asserted-by": "crossref",
      "first-page": "4447",
      "DOI": "10.1073/pnas.0809504106",
      "article-title": "Broad spectrum <italic>O</italic>-linked protein glycosylation in the human pathogen <italic>Neisseria gonorrhoeae</italic>.",
      "volume": "106",
      "author": "A Vik",
      "year": "2009",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref17",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1016/j.cell.2009.02.041",
      "article-title": "A general <italic>O</italic>-glycosylation system important to the physiology of a major human intestinal symbiont.",
      "volume": "137",
      "author": "CM Fletcher",
      "year": "2009",
      "journal-title": "Cell"
    },
    {
      "key": "ref18",
      "doi-asserted-by": "crossref",
      "first-page": "967",
      "DOI": "10.1038/sj.emboj.7601024",
      "article-title": "Two distinct but interchangeable mechanisms for flipping of lipid-linked oligosaccharides.",
      "volume": "25",
      "author": "C Alaimo",
      "year": "2006",
      "journal-title": "Embo J"
    },
    {
      "key": "ref19",
      "doi-asserted-by": "crossref",
      "first-page": "8088",
      "DOI": "10.1128/JB.01318-07",
      "article-title": "Functional characterization of bacterial oligosaccharyltransferases involved in <italic>O</italic>-linked protein glycosylation.",
      "volume": "189",
      "author": "A Faridmoayer",
      "year": "2007",
      "journal-title": "J Bacteriol"
    },
    {
      "key": "ref20",
      "doi-asserted-by": "crossref",
      "first-page": "M000031",
      "DOI": "10.1074/mcp.M000031-MCP201",
      "article-title": "Simultaneous glycan-peptide characterization using hydrophilic interaction chromatography and parallel fragmentation by CID, higher energy collisional dissociation, and electron transfer dissociation MS applied to the <italic>N</italic>-linked glycoproteome of <italic>Campylobacter jejuni</italic>.",
      "volume": "10",
      "author": "NE Scott",
      "year": "2011",
      "journal-title": "Mol Cell Proteomics"
    },
    {
      "key": "ref21",
      "doi-asserted-by": "crossref",
      "first-page": "904",
      "DOI": "10.1016/j.bbrc.2006.06.182",
      "article-title": "Pilin glycosylation in <italic>Neisseria meningitidis</italic> occurs by a similar pathway to wzy-dependent <italic>O</italic>-antigen biosynthesis in <italic>Escherichia coli</italic>.",
      "volume": "347",
      "author": "PM Power",
      "year": "2006",
      "journal-title": "Biochem Biophys Res Commun"
    },
    {
      "key": "ref22",
      "article-title": "Characterization of exogenous bacterial oligosaccharyltransferases in <italic>Escherichia coli</italic> reveals the potential for <italic>O</italic>-linked protein glycosylation in <italic>Vibrio cholerae</italic> and <italic>Burkholderia thailandensis</italic>. Glycobiology.",
      "author": "C Gebhart",
      "year": "2012"
    },
    {
      "key": "ref23",
      "doi-asserted-by": "crossref",
      "first-page": "138",
      "DOI": "10.1093/glycob/cwq148",
      "article-title": "Analogies and homologies in lipopolysaccharide and glycoprotein biosynthesis in bacteria.",
      "volume": "21",
      "author": "I Hug",
      "year": "2011",
      "journal-title": "Glycobiology"
    },
    {
      "key": "ref24",
      "doi-asserted-by": "crossref",
      "first-page": "D396",
      "DOI": "10.1093/nar/gkp919",
      "article-title": "MicrobesOnline: an integrated portal for comparative and functional genomics.",
      "volume": "38",
      "author": "PS Dehal",
      "year": "2010",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref25",
      "doi-asserted-by": "crossref",
      "first-page": "1245",
      "DOI": "10.1128/JCM.36.5.1245-1250.1998",
      "article-title": "Specificity of rabbit antisera against lipopolysaccharide of <italic>Acinetobacter</italic>.",
      "volume": "36",
      "author": "R Pantophlet",
      "year": "1998",
      "journal-title": "J Clin Microbiol"
    },
    {
      "key": "ref26",
      "doi-asserted-by": "crossref",
      "first-page": "3399",
      "DOI": "10.1021/pr101299j",
      "article-title": "Proteomic and Functional Analyses Reveal a Unique Lifestyle for <italic>Acinetobacter baumannii</italic> Biofilms and a Key Role for Histidine Metabolism.",
      "volume": "10",
      "author": "MP Cabral",
      "year": "2011",
      "journal-title": "J Proteome Res"
    },
    {
      "key": "ref27",
      "doi-asserted-by": "crossref",
      "first-page": "2695",
      "DOI": "10.1002/pmic.200800780",
      "article-title": "First evidence of a membrane-bound, tyramine and beta-phenylethylamine producing, tyrosine decarboxylase in <italic>Enterococcus faecalis</italic>: a two-dimensional electrophoresis proteomic study.",
      "volume": "9",
      "author": "E Pessione",
      "year": "2009",
      "journal-title": "Proteomics"
    },
    {
      "key": "ref28",
      "doi-asserted-by": "crossref",
      "first-page": "273",
      "DOI": "10.2217/fmb.09.5",
      "article-title": "Regulation of <italic>Acinetobacter baumannii</italic> biofilm formation.",
      "volume": "4",
      "author": "JA Gaddy",
      "year": "2009",
      "journal-title": "Future Microbiol"
    },
    {
      "key": "ref29",
      "doi-asserted-by": "crossref",
      "first-page": "1015",
      "DOI": "10.1128/IAI.06279-11",
      "article-title": "Role of Acinetobactin-Mediated Iron Acquisition Functions in the Interaction of <italic>Acinetobacter baumannii</italic> Strain ATCC 19606T with Human Lung Epithelial Cells, <italic>Galleria mellonella</italic> Caterpillars, and Mice.",
      "volume": "80",
      "author": "JA Gaddy",
      "year": "2012",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref30",
      "doi-asserted-by": "crossref",
      "first-page": "470",
      "DOI": "10.1099/jmm.0.037523-0",
      "article-title": "Virulence of strains of <italic>Acinetobacter baumannii</italic> is enhanced in <italic>Galleria mellonella</italic> following biofilm adaptation.",
      "volume": "61",
      "author": "M Wand",
      "year": "2011",
      "journal-title": "J Med Microbiol"
    },
    {
      "key": "ref31",
      "doi-asserted-by": "crossref",
      "first-page": "e22674",
      "DOI": "10.1371/journal.pone.0022674",
      "article-title": "Deciphering the multifactorial nature of <italic>Acinetobacter baumannii</italic> pathogenicity.",
      "volume": "6",
      "author": "LC Antunes",
      "year": "2011",
      "journal-title": "PLoS One"
    },
    {
      "key": "ref32",
      "doi-asserted-by": "crossref",
      "first-page": "2605",
      "DOI": "10.1128/AAC.01533-08",
      "article-title": "<italic>Galleria mellonella</italic> as a model system to study <italic>Acinetobacter baumannii</italic> pathogenesis and therapeutics.",
      "volume": "53",
      "author": "AY Peleg",
      "year": "2009",
      "journal-title": "Antimicrob Agents Chemother"
    },
    {
      "key": "ref33",
      "doi-asserted-by": "crossref",
      "first-page": "3534",
      "DOI": "10.1128/AAC.00230-11",
      "article-title": "<italic>In vivo</italic> efficacy of glycopeptide-colistin combination therapies in a <italic>Galleria mellonella</italic> model of <italic>Acinetobacter baumannii</italic> infection.",
      "volume": "55",
      "author": "M Hornsey",
      "year": "2011",
      "journal-title": "Antimicrob Agents Chemother"
    },
    {
      "key": "ref34",
      "doi-asserted-by": "crossref",
      "first-page": "2079",
      "DOI": "10.1128/IAI.01233-10",
      "article-title": "<italic>Dictyostelium discoideum</italic> as a model system for identification of <italic>Burkholderia pseudomallei</italic> virulence factors.",
      "volume": "79",
      "author": "BM Hasselbring",
      "year": "2011",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref35",
      "doi-asserted-by": "crossref",
      "first-page": "3843",
      "DOI": "10.1128/JB.182.13.3843-3845.2000",
      "article-title": "Positive correlation between virulence of <italic>Pseudomonas aeruginosa</italic> mutants in mice and insects.",
      "volume": "182",
      "author": "G Jander",
      "year": "2000",
      "journal-title": "J Bacteriol"
    },
    {
      "key": "ref36",
      "doi-asserted-by": "crossref",
      "first-page": "3842",
      "DOI": "10.1128/IAI.73.7.3842-3850.2005",
      "article-title": "<italic>Galleria mellonella</italic> as a model system to study <italic>Cryptococcus neoformans</italic> pathogenesis.",
      "volume": "73",
      "author": "E Mylonakis",
      "year": "2005",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref37",
      "doi-asserted-by": "crossref",
      "first-page": "545",
      "DOI": "10.1093/infdis/jiq086",
      "article-title": "Impaired virulence and <italic>in vivo</italic> fitness of colistin-resistant <italic>Acinetobacter baumannii</italic>.",
      "volume": "203",
      "author": "R Lopez-Rojas",
      "year": "2010",
      "journal-title": "J Infect Dis"
    },
    {
      "key": "ref38",
      "doi-asserted-by": "crossref",
      "first-page": "E1",
      "DOI": "10.1111/j.1469-0691.2011.03695.x",
      "article-title": "<italic>In vitro</italic> and <italic>in vivo</italic> reduced fitness and virulence in ciprofloxacin-resistant <italic>Acinetobacter baumannii</italic>.",
      "volume": "18",
      "author": "Y Smani",
      "year": "2011",
      "journal-title": "Clin Microbiol Infect"
    },
    {
      "key": "ref39",
      "doi-asserted-by": "crossref",
      "first-page": "4275",
      "DOI": "10.1128/IAI.72.7.4275-4278.2004",
      "article-title": "<italic>Pseudomonas aeruginosa</italic> pyocyanin is critical for lung infection in mice.",
      "volume": "72",
      "author": "GW Lau",
      "year": "2004",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref40",
      "doi-asserted-by": "crossref",
      "first-page": "13758",
      "DOI": "10.1073/pnas.1104404108",
      "article-title": "Genome-wide recombination drives diversification of epidemic strains of <italic>Acinetobacter baumannii</italic>.",
      "volume": "108",
      "author": "ES Snitkin",
      "year": "2011",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref41",
      "doi-asserted-by": "crossref",
      "first-page": "2242",
      "DOI": "10.1128/IAI.70.4.2242-2244.2002",
      "article-title": "<italic>Campylobacter</italic> protein glycosylation affects host cell interactions.",
      "volume": "70",
      "author": "CM Szymanski",
      "year": "2002",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref42",
      "doi-asserted-by": "crossref",
      "first-page": "2816",
      "DOI": "10.1128/JB.00101-10",
      "article-title": "Genetic, structural, and antigenic analyses of glycan diversity in the <italic>O</italic>-linked protein glycosylation systems of human <italic>Neisseria</italic> species.",
      "volume": "192",
      "author": "B Borud",
      "year": "2011",
      "journal-title": "J Bacteriol"
    },
    {
      "key": "ref43",
      "doi-asserted-by": "crossref",
      "first-page": "885",
      "DOI": "10.1111/j.1462-5822.2011.01586.x",
      "article-title": "<italic>Neisseria gonorrhoeae</italic> pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells.",
      "volume": "13",
      "author": "MP Jennings",
      "year": "2011",
      "journal-title": "Cell Microbiol"
    },
    {
      "key": "ref44",
      "doi-asserted-by": "crossref",
      "first-page": "3219",
      "DOI": "10.1074/jbc.M110.194506",
      "article-title": "Theoretical and experimental characterization of the scope of protein <italic>O</italic>-glycosylation in <italic>Bacteroides fragilis</italic>.",
      "volume": "286",
      "author": "CM Fletcher",
      "year": "2011",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref45",
      "first-page": "1784",
      "article-title": "[Structure of lipopolysaccharides from gram-negative bacteria. III. Structure of O-specific polysaccharides].",
      "volume": "59",
      "author": "YA Knirel",
      "year": "1994",
      "journal-title": "Biokhimiia"
    },
    {
      "key": "ref46",
      "doi-asserted-by": "crossref",
      "first-page": "2122",
      "DOI": "10.1021/bi052227i",
      "article-title": "Peptidoglycan recognition by Pal, an outer membrane lipoprotein.",
      "volume": "45",
      "author": "LM Parsons",
      "year": "2006",
      "journal-title": "Biochemistry"
    },
    {
      "key": "ref47",
      "doi-asserted-by": "crossref",
      "first-page": "24",
      "DOI": "10.1016/j.jtbi.2007.10.039",
      "article-title": "Microcolony and biofilm formation as a survival strategy for bacteria.",
      "volume": "251",
      "author": "LR Johnson",
      "year": "2008",
      "journal-title": "J Theor Biol"
    },
    {
      "key": "ref48",
      "doi-asserted-by": "crossref",
      "first-page": "213",
      "DOI": "10.1007/978-94-007-0940-9_13",
      "article-title": "Bacterial extracellular polysaccharides.",
      "volume": "715",
      "author": "K Bazaka",
      "year": "2011",
      "journal-title": "Adv Exp Med Biol"
    },
    {
      "key": "ref49",
      "doi-asserted-by": "crossref",
      "first-page": "55",
      "DOI": "10.4248/IJOS11026",
      "article-title": "The clinical impact of bacterial biofilms.",
      "volume": "3",
      "author": "N Hoiby",
      "year": "2011",
      "journal-title": "Int J Oral Sci"
    },
    {
      "key": "ref50",
      "doi-asserted-by": "crossref",
      "first-page": "1140",
      "DOI": "10.1128/jb.184.4.1140-1154.2002",
      "article-title": "<italic>Pseudomonas aeruginosa</italic> displays multiple phenotypes during development as a biofilm.",
      "volume": "184",
      "author": "K Sauer",
      "year": "2002",
      "journal-title": "J Bacteriol"
    },
    {
      "key": "ref51",
      "doi-asserted-by": "crossref",
      "first-page": "295",
      "DOI": "10.1046/j.1365-2958.1998.01062.x",
      "article-title": "Flagellar and twitching motility are necessary for <italic>Pseudomonas aeruginosa</italic> biofilm development.",
      "volume": "30",
      "author": "GA O'Toole",
      "year": "1998",
      "journal-title": "Mol Microbiol"
    },
    {
      "key": "ref52",
      "doi-asserted-by": "crossref",
      "first-page": "271",
      "DOI": "10.1016/j.mib.2008.05.005",
      "article-title": "Eat, kill or die: when amoeba meets bacteria.",
      "volume": "11",
      "author": "P Cosson",
      "year": "2008",
      "journal-title": "Curr Opin Microbiol"
    },
    {
      "key": "ref53",
      "doi-asserted-by": "crossref",
      "first-page": "668",
      "DOI": "10.1128/IAI.64.2.668-673.1996",
      "article-title": "Interaction of <italic>Legionella pneumophila</italic> with <italic>Acanthamoeba castellanii</italic>: uptake by coiling phagocytosis and inhibition of phagosome-lysosome fusion.",
      "volume": "64",
      "author": "JA Bozue",
      "year": "1996",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref54",
      "doi-asserted-by": "crossref",
      "first-page": "101",
      "DOI": "10.1016/j.femsre.2003.09.002",
      "article-title": "Exploiting the potential of insects for <italic>in vivo</italic> pathogenicity testing of microbial pathogens.",
      "volume": "28",
      "author": "K Kavanagh",
      "year": "2004",
      "journal-title": "FEMS Microbiol Rev"
    },
    {
      "key": "ref55",
      "doi-asserted-by": "crossref",
      "first-page": "5716",
      "DOI": "10.1128/IAI.68.10.5716-5723.2000",
      "article-title": "Identification and characterization of the <italic>Brucella abortus</italic> phosphoglucomutase gene: role of lipopolysaccharide in virulence and intracellular multiplication.",
      "volume": "68",
      "author": "JE Ugalde",
      "year": "2000",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref56",
      "doi-asserted-by": "crossref",
      "first-page": "160",
      "DOI": "10.1016/j.vetmic.2007.06.007",
      "article-title": "Glycosylation of type-IV fimbriae of <italic>Dichelobacter nodosus</italic>.",
      "volume": "126",
      "author": "TI Cagatay",
      "year": "2008",
      "journal-title": "Vet Microbiol"
    },
    {
      "key": "ref57",
      "first-page": "237",
      "article-title": "Micromethods for the characterization of lipid A-core and O-antigen lipopolysaccharide.",
      "volume": "347",
      "author": "CL Marolda",
      "year": "2006",
      "journal-title": "Methods Mol Biol"
    },
    {
      "key": "ref58",
      "doi-asserted-by": "crossref",
      "first-page": "164",
      "DOI": "10.1002/pmic.200900570",
      "article-title": "The effect of interfering ions on search algorithm performance for electron-transfer dissociation data.",
      "volume": "10",
      "author": "DM Good",
      "year": "2010",
      "journal-title": "Proteomics"
    },
    {
      "key": "ref59",
      "doi-asserted-by": "crossref",
      "first-page": "1435",
      "DOI": "10.1016/j.jasms.2009.03.006",
      "article-title": "Post-acquisition ETD spectral processing for increased peptide identifications.",
      "volume": "20",
      "author": "DM Good",
      "year": "2009",
      "journal-title": "J Am Soc Mass Spectrom"
    },
    {
      "key": "ref60",
      "doi-asserted-by": "crossref",
      "first-page": "1015",
      "DOI": "10.1111/j.1365-2958.2011.07867.x",
      "article-title": "Extracellular nucleases and extracellular DNA play important roles in <italic>Vibrio cholerae</italic> biofilm formation.",
      "volume": "82",
      "author": "A Seper",
      "year": "2011",
      "journal-title": "Mol Microbiol"
    },
    {
      "key": "ref61",
      "doi-asserted-by": "crossref",
      "first-page": "2395",
      "DOI": "10.1099/00221287-146-10-2395",
      "article-title": "Quantification of biofilm structures by the novel computer program COMSTAT.",
      "volume": "10)",
      "author": "A Heydorn",
      "year": "2000",
      "journal-title": "Microbiology 146 ( Pt"
    },
    {
      "key": "ref62",
      "doi-asserted-by": "crossref",
      "first-page": "3159",
      "DOI": "10.1073/pnas.052704399",
      "article-title": "The human pathogen <italic>Pseudomonas aeruginosa</italic> utilizes conserved virulence pathways to infect the social amoeba <italic>Dictyostelium discoideum</italic>.",
      "volume": "99",
      "author": "S Pukatzki",
      "year": "2002",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref63",
      "doi-asserted-by": "crossref",
      "first-page": "393",
      "DOI": "10.1093/jac/dkh080",
      "article-title": "<italic>In vitro</italic> and <italic>in vivo</italic> activity of meropenem and sulbactam against a multidrug-resistant <italic>Acinetobacter baumannii</italic> strain.",
      "volume": "53",
      "author": "WC Ko",
      "year": "2004",
      "journal-title": "J Antimicrob Chemother"
    },
    {
      "key": "ref64",
      "doi-asserted-by": "crossref",
      "first-page": "402",
      "DOI": "10.1016/j.micpath.2011.09.004",
      "article-title": "Protection against <italic>Acinetobacter baumannii</italic> infection via its functional deprivation of biofilm associated protein (Bap).",
      "volume": "51",
      "author": "Y Fattahian",
      "year": "2011",
      "journal-title": "Microb Pathog"
    },
    {
      "key": "ref65",
      "doi-asserted-by": "crossref",
      "first-page": "518",
      "DOI": "10.1128/IAI.00741-10",
      "article-title": "Vaccination with outer membrane complexes elicits rapid protective immunity to multidrug-resistant <italic>Acinetobacter baumannii</italic>.",
      "volume": "79",
      "author": "MJ McConnell",
      "year": "2011",
      "journal-title": "Infect Immun"
    },
    {
      "key": "ref66",
      "doi-asserted-by": "crossref",
      "first-page": "231",
      "DOI": "10.1084/jem.91.2.231",
      "article-title": "The virulence and immunogenicity of strains derived from <italic>Salmonella typhosa</italic> 58 (Panama carrier).",
      "volume": "91",
      "author": "HC Batson",
      "year": "1950",
      "journal-title": "J Exp Med"
    },
    {
      "key": "ref67",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/j.vaccine.2010.10.052",
      "article-title": "Active and passive immunization against <italic>Acinetobacter baumannii</italic> using an inactivated whole cell vaccine.",
      "volume": "29",
      "author": "MJ McConnell",
      "year": "2010",
      "journal-title": "Vaccine"
    },
    {
      "key": "ref68",
      "first-page": "97",
      "article-title": "[Studies of 26 strains of Moraxella Iwoffi].",
      "volume": "80",
      "author": "M Piechaud",
      "year": "1951",
      "journal-title": "Ann Inst Pasteur (Paris)"
    },
    {
      "key": "ref69",
      "doi-asserted-by": "crossref",
      "first-page": "133",
      "DOI": "10.1016/0378-1119(96)00289-2",
      "article-title": "A set of compatible tac promoter expression vectors.",
      "volume": "177",
      "author": "DM Dykxhoorn",
      "year": "1996",
      "journal-title": "Gene"
    },
    {
      "key": "ref70",
      "doi-asserted-by": "crossref",
      "first-page": "45",
      "DOI": "10.1016/0378-1119(90)90494-C",
      "article-title": "Analysis and nucleotide sequence of an origin of DNA replication in <italic>Acinetobacter calcoaceticus</italic> and its use for <italic>Escherichia coli</italic> shuttle plasmids.",
      "volume": "87",
      "author": "M Hunger",
      "year": "1990",
      "journal-title": "Gene"
    },
    {
      "key": "ref71",
      "doi-asserted-by": "crossref",
      "first-page": "77",
      "DOI": "10.1016/S0378-1119(98)00130-9",
      "article-title": "A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked <italic>Pseudomonas aeruginosa</italic> mutants.",
      "volume": "212",
      "author": "TT Hoang",
      "year": "1998",
      "journal-title": "Gene"
    }
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