DOI: 10.1371/journal.ppat.1000591. Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages

Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages

10.1371/journal.ppat.1000591

Crossref journal-article

Public Library of Science (PLoS)

PLoS Pathogens (340)

Bibliography

Bennett, A. E., Narayan, K., Shi, D., Hartnell, L. M., Gousset, K., He, H., Lowekamp, B. C., Yoo, T. S., Bliss, D., Freed, E. O., & Subramaniam, S. (2009). Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages. PLoS Pathogens, 5(9), e1000591.

Authors 11

Adam E. Bennett (first)
Kedar Narayan (additional)
Dan Shi (additional)
Lisa M. Hartnell (additional)
Karine Gousset (additional)
Haifeng He (additional)
Bradley C. Lowekamp (additional)
Terry S. Yoo (additional)
Donald Bliss (additional)
Eric O. Freed (additional)
Sriram Subramaniam (additional)

References 30 Referenced 139

10.1080/01913120050118530 / Ultrastruct Pathol / Isn't a picture still worth a thousand words? by JM Orenstein (2000)
10.1016/j.jsb.2006.03.006 / J Struct Biol / Site-specific 3D imaging of cells and tissues with a dual beam microscope. by JA Heymann (2006)
10.1016/j.jsb.2008.11.005 / J Struct Biol / 3D imaging of mammalian cells with ion-abrasion scanning electron microscopy. by JA Heymann (2009)
10.1016/j.jsb.2009.02.014 / J Struct Biol / 3D imaging of diatoms with ion-abrasion scanning electron microscopy. by M Hildebrand (2009)
10.1371/journal.ppat.1000015 / PLoS Pathog / Real-time visualization of HIV-1 GAG trafficking in infected macrophages. by K Gousset (2008)
10.1371/journal.pbio.0040435 / PLoS Biol / Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly. by N Jouvenet (2006)
10.1371/journal.ppat.0030036 / PLoS Pathog / HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages. by S Welsch (2007)
10.1002/jmv.10245 / J Med Virol / Long-term survival and virus production in human primary macrophages infected by human immunodeficiency virus. by S Aquaro (2002)
10.1189/jlb.0206126 / J Leukoc Biol / In vitro modeling of the HIV-macrophage reservoir. by A Brown (2006)
10.1073/pnas.98.2.658 / Proc Natl Acad Sci U S A / Macrophage are the principal reservoir and sustain high virus loads in rhesus macaques after the depletion of CD4+ T cells by a highly pathogenic simian immunodeficiency virus/HIV type 1 chimera (SHIV): Implications for HIV-1 infections of humans. by T Igarashi (2001)
10.1038/sj.emboj.7600707 / Embo J / Macrophages archive HIV-1 virions for dissemination in trans. by N Sharova (2005)
10.1189/jlb.0503219 / J Leukoc Biol / Macrophage HIV-1 infection and the gastrointestinal tract reservoir. by PD Smith (2003)
10.1006/viro.1999.0047 / Virology / Rapid and efficient cell-to-cell transmission of human immunodeficiency virus infection from monocyte-derived macrophages to peripheral blood lymphocytes. by JM Carr (1999)
10.1083/jcb.200304008 / J Cell Biol / Infectious HIV-1 assembles in late endosomes in primary macrophages. by A Pelchen-Matthews (2003)
10.1083/jcb.200609050 / J Cell Biol / In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53. by M Deneka (2007)
10.1073/pnas.89.1.70 / Proc Natl Acad Sci U S A / A mutation in the human immunodeficiency virus type 1 transmembrane glycoprotein gp41 dominantly interferes with fusion and infectivity. by EO Freed (1992)
{'key': 'ref17', 'first-page': '99', 'article-title': 'Generation of high-titer pseudotyped retroviral vectors with very broad host range.', 'volume': '43 Pt A', 'author': 'JK Yee', 'year': '1994', 'journal-title': 'Methods Cell Biol'} / Methods Cell Biol / Generation of high-titer pseudotyped retroviral vectors with very broad host range. by JK Yee (1994)
10.1006/jsbi.1997.3919 / J Struct Biol / Dual-axis tomography: an approach with alignment methods that preserve resolution. by DN Mastronarde (1997)
10.1006/jsbi.1996.0013 / J Struct Biol / Computer visualization of three-dimensional image data using IMOD. by JR Kremer (1996)
10.1046/j.1365-2818.2003.01238.x / J Microsc / Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography. by JL Murk (2003)
10.1128/JVI.00109-09 / J Virol / Evidence that productive human immunodeficiency virus type 1 assembly can occur in an intracellular compartment. by A Joshi (2009)
10.1073/pnas.88.18.7998 / Proc Natl Acad Sci U S A / Replication of human immunodeficiency virus type 1 in primary dendritic cell cultures. by E Langhoff (1991)
10.1177/25.9.333019 / J Histochem Cytochem / Immune complex receptors on cell surfaces. III. Topography of macrophage receptors demonstrated by new scanning electron microscopic peroxidase marker. by PE McKeever (1977)
10.1002/jlb.48.3.237 / J Leukoc Biol / Lysis of autologous human macrophages by lymphokine-activated killer cells: interaction of effector cell and target cell conjugates analyzed by scanning electron microscopy. by RJ Streck (1990)
10.1002/(SICI)1097-4636(199801)39:1<40::AID-JBM6>3.0.CO;2-I / J Biomed Mater Res / Macrophage phagocytosis of polyethylene particulate in vitro. by I Voronov (1998)
{'key': 'ref26', 'first-page': '739', 'article-title': 'Gastric oxyntic cell structure as related to secretory activity.', 'volume': '12', 'author': 'T Ogata', 'year': '1997', 'journal-title': 'Histol Histopathol'} / Histol Histopathol / Gastric oxyntic cell structure as related to secretory activity. by T Ogata (1997)
10.1083/jcb.200107075 / J Cell Biol / Generation of high curvature membranes mediated by direct endophilin bilayer interactions. by K Farsad (2001)
10.1038/nature01006 / Nature / Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane. by A Chow (2002)
10.1083/jcb.200103071 / J Cell Biol / Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. by M Kleijmeer (2001)
10.1016/j.patcog.2008.08.009 / Pattern Recognition / Automatic joint classification and segmentation of whole cell 3D images. by R Narasimha (2008)

Dates

Type	When
Created	15 years, 10 months ago (Sept. 25, 2009, 8:13 a.m.)
Deposited	5 years, 3 months ago (May 9, 2020, 6:45 a.m.)
Indexed	3 weeks, 6 days ago (July 25, 2025, 6:30 a.m.)
Issued	15 years, 10 months ago (Sept. 25, 2009)
Published	15 years, 10 months ago (Sept. 25, 2009)
Published Online	15 years, 10 months ago (Sept. 25, 2009)

Funders 0

None

BibTeX

@article{Bennett_2009, title={Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages}, volume={5}, ISSN={1553-7374}, url={http://dx.doi.org/10.1371/journal.ppat.1000591}, DOI={10.1371/journal.ppat.1000591}, number={9}, journal={PLoS Pathogens}, publisher={Public Library of Science (PLoS)}, author={Bennett, Adam E. and Narayan, Kedar and Shi, Dan and Hartnell, Lisa M. and Gousset, Karine and He, Haifeng and Lowekamp, Bradley C. and Yoo, Terry S. and Bliss, Donald and Freed, Eric O. and Subramaniam, Sriram}, editor={Hope, Thomas J.}, year={2009}, month=sep, pages={e1000591} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        25
      ]
    ],
    "date-time": "2025-07-25T10:30:59Z",
    "timestamp": 1753439459679
  },
  "reference-count": 30,
  "publisher": "Public Library of Science (PLoS)",
  "issue": "9",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2009,
            9,
            25
          ]
        ],
        "date-time": "2009-09-25T00:00:00Z",
        "timestamp": 1253836800000
      },
      "content-version": "unspecified",
      "delay-in-days": 0,
      "URL": "https://creativecommons.org/publicdomain/zero/1.0/"
    }
  ],
  "content-domain": {
    "domain": [
      "www.plospathogens.org"
    ],
    "crossmark-restriction": false
  },
  "DOI": "10.1371/journal.ppat.1000591",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        9,
        25
      ]
    ],
    "date-time": "2009-09-25T12:13:53Z",
    "timestamp": 1253880833000
  },
  "page": "e1000591",
  "update-policy": "http://dx.doi.org/10.1371/journal.ppat.corrections_policy",
  "source": "Crossref",
  "is-referenced-by-count": 139,
  "title": "Ion-Abrasion Scanning Electron Microscopy Reveals Surface-Connected Tubular Conduits in HIV-Infected Macrophages",
  "prefix": "10.1371",
  "volume": "5",
  "author": [
    {
      "given": "Adam E.",
      "family": "Bennett",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Kedar",
      "family": "Narayan",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Dan",
      "family": "Shi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Lisa M.",
      "family": "Hartnell",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Karine",
      "family": "Gousset",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Haifeng",
      "family": "He",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Bradley C.",
      "family": "Lowekamp",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Terry S.",
      "family": "Yoo",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Donald",
      "family": "Bliss",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Eric O.",
      "family": "Freed",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Sriram",
      "family": "Subramaniam",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "340",
  "published-online": {
    "date-parts": [
      [
        2009,
        9,
        25
      ]
    ]
  },
  "reference": [
    {
      "key": "ref1",
      "doi-asserted-by": "crossref",
      "first-page": "67",
      "DOI": "10.1080/01913120050118530",
      "article-title": "Isn&apos;t a picture still worth a thousand words?",
      "volume": "24",
      "author": "JM Orenstein",
      "year": "2000",
      "journal-title": "Ultrastruct Pathol"
    },
    {
      "key": "ref2",
      "doi-asserted-by": "crossref",
      "first-page": "63",
      "DOI": "10.1016/j.jsb.2006.03.006",
      "article-title": "Site-specific 3D imaging of cells and tissues with a dual beam microscope.",
      "volume": "155",
      "author": "JA Heymann",
      "year": "2006",
      "journal-title": "J Struct Biol"
    },
    {
      "key": "ref3",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/j.jsb.2008.11.005",
      "article-title": "3D imaging of mammalian cells with ion-abrasion scanning electron microscopy.",
      "volume": "166",
      "author": "JA Heymann",
      "year": "2009",
      "journal-title": "J Struct Biol"
    },
    {
      "key": "ref4",
      "doi-asserted-by": "crossref",
      "first-page": "316",
      "DOI": "10.1016/j.jsb.2009.02.014",
      "article-title": "3D imaging of diatoms with ion-abrasion scanning electron microscopy.",
      "volume": "166",
      "author": "M Hildebrand",
      "year": "2009",
      "journal-title": "J Struct Biol"
    },
    {
      "key": "ref5",
      "doi-asserted-by": "crossref",
      "first-page": "e1000015",
      "DOI": "10.1371/journal.ppat.1000015",
      "article-title": "Real-time visualization of HIV-1 GAG trafficking in infected macrophages.",
      "volume": "4",
      "author": "K Gousset",
      "year": "2008",
      "journal-title": "PLoS Pathog"
    },
    {
      "key": "ref6",
      "doi-asserted-by": "crossref",
      "first-page": "e435",
      "DOI": "10.1371/journal.pbio.0040435",
      "article-title": "Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly.",
      "volume": "4",
      "author": "N Jouvenet",
      "year": "2006",
      "journal-title": "PLoS Biol"
    },
    {
      "key": "ref7",
      "doi-asserted-by": "crossref",
      "first-page": "e36",
      "DOI": "10.1371/journal.ppat.0030036",
      "article-title": "HIV-1 Buds Predominantly at the Plasma Membrane of Primary Human Macrophages.",
      "volume": "3",
      "author": "S Welsch",
      "year": "2007",
      "journal-title": "PLoS Pathog"
    },
    {
      "key": "ref8",
      "doi-asserted-by": "crossref",
      "first-page": "479",
      "DOI": "10.1002/jmv.10245",
      "article-title": "Long-term survival and virus production in human primary macrophages infected by human immunodeficiency virus.",
      "volume": "68",
      "author": "S Aquaro",
      "year": "2002",
      "journal-title": "J Med Virol"
    },
    {
      "key": "ref9",
      "doi-asserted-by": "crossref",
      "first-page": "1127",
      "DOI": "10.1189/jlb.0206126",
      "article-title": "In vitro modeling of the HIV-macrophage reservoir.",
      "volume": "80",
      "author": "A Brown",
      "year": "2006",
      "journal-title": "J Leukoc Biol"
    },
    {
      "key": "ref10",
      "doi-asserted-by": "crossref",
      "first-page": "658",
      "DOI": "10.1073/pnas.98.2.658",
      "article-title": "Macrophage are the principal reservoir and sustain high virus loads in rhesus macaques after the depletion of CD4+ T cells by a highly pathogenic simian immunodeficiency virus/HIV type 1 chimera (SHIV): Implications for HIV-1 infections of humans.",
      "volume": "98",
      "author": "T Igarashi",
      "year": "2001",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref11",
      "doi-asserted-by": "crossref",
      "first-page": "2481",
      "DOI": "10.1038/sj.emboj.7600707",
      "article-title": "Macrophages archive HIV-1 virions for dissemination in trans.",
      "volume": "24",
      "author": "N Sharova",
      "year": "2005",
      "journal-title": "Embo J"
    },
    {
      "key": "ref12",
      "doi-asserted-by": "crossref",
      "first-page": "642",
      "DOI": "10.1189/jlb.0503219",
      "article-title": "Macrophage HIV-1 infection and the gastrointestinal tract reservoir.",
      "volume": "74",
      "author": "PD Smith",
      "year": "2003",
      "journal-title": "J Leukoc Biol"
    },
    {
      "key": "ref13",
      "doi-asserted-by": "crossref",
      "first-page": "319",
      "DOI": "10.1006/viro.1999.0047",
      "article-title": "Rapid and efficient cell-to-cell transmission of human immunodeficiency virus infection from monocyte-derived macrophages to peripheral blood lymphocytes.",
      "volume": "265",
      "author": "JM Carr",
      "year": "1999",
      "journal-title": "Virology"
    },
    {
      "key": "ref14",
      "doi-asserted-by": "crossref",
      "first-page": "443",
      "DOI": "10.1083/jcb.200304008",
      "article-title": "Infectious HIV-1 assembles in late endosomes in primary macrophages.",
      "volume": "162",
      "author": "A Pelchen-Matthews",
      "year": "2003",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "ref15",
      "doi-asserted-by": "crossref",
      "first-page": "329",
      "DOI": "10.1083/jcb.200609050",
      "article-title": "In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53.",
      "volume": "177",
      "author": "M Deneka",
      "year": "2007",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "ref16",
      "doi-asserted-by": "crossref",
      "first-page": "70",
      "DOI": "10.1073/pnas.89.1.70",
      "article-title": "A mutation in the human immunodeficiency virus type 1 transmembrane glycoprotein gp41 dominantly interferes with fusion and infectivity.",
      "volume": "89",
      "author": "EO Freed",
      "year": "1992",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref17",
      "first-page": "99",
      "article-title": "Generation of high-titer pseudotyped retroviral vectors with very broad host range.",
      "volume": "43 Pt A",
      "author": "JK Yee",
      "year": "1994",
      "journal-title": "Methods Cell Biol"
    },
    {
      "key": "ref18",
      "doi-asserted-by": "crossref",
      "first-page": "343",
      "DOI": "10.1006/jsbi.1997.3919",
      "article-title": "Dual-axis tomography: an approach with alignment methods that preserve resolution.",
      "volume": "120",
      "author": "DN Mastronarde",
      "year": "1997",
      "journal-title": "J Struct Biol"
    },
    {
      "key": "ref19",
      "doi-asserted-by": "crossref",
      "first-page": "71",
      "DOI": "10.1006/jsbi.1996.0013",
      "article-title": "Computer visualization of three-dimensional image data using IMOD.",
      "volume": "116",
      "author": "JR Kremer",
      "year": "1996",
      "journal-title": "J Struct Biol"
    },
    {
      "key": "ref20",
      "doi-asserted-by": "crossref",
      "first-page": "81",
      "DOI": "10.1046/j.1365-2818.2003.01238.x",
      "article-title": "Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography.",
      "volume": "212",
      "author": "JL Murk",
      "year": "2003",
      "journal-title": "J Microsc"
    },
    {
      "key": "ref21",
      "doi-asserted-by": "crossref",
      "first-page": "5375",
      "DOI": "10.1128/JVI.00109-09",
      "article-title": "Evidence that productive human immunodeficiency virus type 1 assembly can occur in an intracellular compartment.",
      "volume": "83",
      "author": "A Joshi",
      "year": "2009",
      "journal-title": "J Virol"
    },
    {
      "key": "ref22",
      "doi-asserted-by": "crossref",
      "first-page": "7998",
      "DOI": "10.1073/pnas.88.18.7998",
      "article-title": "Replication of human immunodeficiency virus type 1 in primary dendritic cell cultures.",
      "volume": "88",
      "author": "E Langhoff",
      "year": "1991",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref23",
      "doi-asserted-by": "crossref",
      "first-page": "1063",
      "DOI": "10.1177/25.9.333019",
      "article-title": "Immune complex receptors on cell surfaces. III. Topography of macrophage receptors demonstrated by new scanning electron microscopic peroxidase marker.",
      "volume": "25",
      "author": "PE McKeever",
      "year": "1977",
      "journal-title": "J Histochem Cytochem"
    },
    {
      "key": "ref24",
      "doi-asserted-by": "crossref",
      "first-page": "237",
      "DOI": "10.1002/jlb.48.3.237",
      "article-title": "Lysis of autologous human macrophages by lymphokine-activated killer cells: interaction of effector cell and target cell conjugates analyzed by scanning electron microscopy.",
      "volume": "48",
      "author": "RJ Streck",
      "year": "1990",
      "journal-title": "J Leukoc Biol"
    },
    {
      "key": "ref25",
      "doi-asserted-by": "crossref",
      "first-page": "40",
      "DOI": "10.1002/(SICI)1097-4636(199801)39:1<40::AID-JBM6>3.0.CO;2-I",
      "article-title": "Macrophage phagocytosis of polyethylene particulate in vitro.",
      "volume": "39",
      "author": "I Voronov",
      "year": "1998",
      "journal-title": "J Biomed Mater Res"
    },
    {
      "key": "ref26",
      "first-page": "739",
      "article-title": "Gastric oxyntic cell structure as related to secretory activity.",
      "volume": "12",
      "author": "T Ogata",
      "year": "1997",
      "journal-title": "Histol Histopathol"
    },
    {
      "key": "ref27",
      "doi-asserted-by": "crossref",
      "first-page": "193",
      "DOI": "10.1083/jcb.200107075",
      "article-title": "Generation of high curvature membranes mediated by direct endophilin bilayer interactions.",
      "volume": "155",
      "author": "K Farsad",
      "year": "2001",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "ref28",
      "doi-asserted-by": "crossref",
      "first-page": "988",
      "DOI": "10.1038/nature01006",
      "article-title": "Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane.",
      "volume": "418",
      "author": "A Chow",
      "year": "2002",
      "journal-title": "Nature"
    },
    {
      "key": "ref29",
      "doi-asserted-by": "crossref",
      "first-page": "53",
      "DOI": "10.1083/jcb.200103071",
      "article-title": "Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells.",
      "volume": "155",
      "author": "M Kleijmeer",
      "year": "2001",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "ref30",
      "doi-asserted-by": "crossref",
      "first-page": "1067",
      "DOI": "10.1016/j.patcog.2008.08.009",
      "article-title": "Automatic joint classification and segmentation of whole cell 3D images.",
      "volume": "42",
      "author": "R Narasimha",
      "year": "2008",
      "journal-title": "Pattern Recognition"
    }
  ],
  "container-title": "PLoS Pathogens",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://dx.plos.org/10.1371/journal.ppat.1000591",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2020,
        5,
        9
      ]
    ],
    "date-time": "2020-05-09T10:45:50Z",
    "timestamp": 1589021150000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://dx.plos.org/10.1371/journal.ppat.1000591"
    }
  },
  "subtitle": [],
  "editor": [
    {
      "given": "Thomas J.",
      "family": "Hope",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2009,
        9,
        25
      ]
    ]
  },
  "references-count": 30,
  "journal-issue": {
    "issue": "9",
    "published-online": {
      "date-parts": [
        [
          2009,
          9,
          25
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1371/journal.ppat.1000591",
  "relation": {},
  "ISSN": [
    "1553-7374"
  ],
  "subject": [],
  "container-title-short": "PLoS Pathog",
  "published": {
    "date-parts": [
      [
        2009,
        9,
        25
      ]
    ]
  }
}