DOI: 10.1371/journal.ppat.1000259. A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75

A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75

10.1371/journal.ppat.1000259

Crossref journal-article

Public Library of Science (PLoS)

PLoS Pathogens (340)

Bibliography

Hare, S., Shun, M.-C., Gupta, S. S., Valkov, E., Engelman, A., & Cherepanov, P. (2009). A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75. PLoS Pathogens, 5(1), e1000259.

Authors 6

Stephen Hare (first)
Ming-Chieh Shun (additional)
Saumya Shree Gupta (additional)
Eugene Valkov (additional)
Alan Engelman (additional)
Peter Cherepanov (additional)

References 62 Referenced 132

10.1016/S0065-2660(05)55005-3 / Adv Genet / Retroviral DNA integration–mechanism and consequences. by MK Lewinski (2005)
{'key': 'ref2', 'first-page': '613', 'article-title': 'Retroviral DNA Integration.', 'author': 'R Craigie', 'year': '2002'} / Retroviral DNA Integration. by R Craigie (2002)
10.1073/pnas.90.8.3428 / Proc Natl Acad Sci USA / Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. by FD Bushman (1993)
10.1002/j.1460-2075.1993.tb05996.x / EMBO J / Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. by A Engelman (1993)
10.1128/JVI.66.11.6361-6369.1992 / J Virol / Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. by A Engelman (1992)
10.1126/science.7801124 / Science / Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. by F Dyda (1994)
10.1073/pnas.95.16.9150 / Proc Natl Acad Sci USA / Three new structures of the core domain of HIV-1 integrase: an active site that binds magnesium. by Y Goldgur (1998)
10.1016/j.molcel.2006.03.013 / Mol Cell / Making and breaking nucleic acids: two-Mg2+−ion catalysis and substrate specificity. by W Yang (2006)
10.1038/nsb0797-567 / Nat Struct Biol / Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. by M Cai (1997)
10.1016/S0960-9822(06)00332-0 / Curr Biol / The solution structure of the amino-terminal HHCC domain of HIV-2 integrase: a three-helix bundle stabilized by zinc. by AP Eijkelenboom (1997)
10.1038/nsb0995-807 / Nat Struct Biol / The DNA-binding domain of HIV-1 integrase has an SH3-like fold. by AP Eijkelenboom (1995)
10.1021/bi00031a002 / Biochemistry (Mosc) / Solution structure of the DNA binding domain of HIV-1 integrase. by PJ Lodi (1995)
10.1074/jbc.C200550200 / J Biol Chem / Functional oligomeric state of avian sarcoma virus integrase. by KK Bao (2003)
10.1093/nar/gki241 / Nucleic Acids Res / HIV-1 integrase crosslinked oligomers are active in vitro. by A Faure (2005)
10.1038/sj.emboj.7601005 / EMBO J / Retroviral DNA integration: reaction pathway and critical intermediates. by M Li (2006)
10.1093/emboj/20.24.7333 / EMBO J / Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein. by JY Wang (2001)
10.1371/journal.ppat.1000046 / PLoS Pathog / The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. by A Engelman (2008)
10.1016/j.ccr.2008.05.003 / Cancer Cell / Menin critically links MLL proteins with LEDGF on cancer-associated target genes. by A Yokoyama (2008)
10.1006/bbrc.2001.4887 / Biochem Biophys Res Commun / LEDGF binds to heat shock and stress-related element to activate the expression of stress-related genes. by DP Singh (2001)
10.1038/sj.cdd.4401063 / Cell Death Differ / Caspase cleavage of the nuclear autoantigen LEDGF/p75 abrogates its pro-survival function: implications for autoimmunity in atopic disorders. by X Wu (2002)
10.1074/jbc.M209278200 / J Biol Chem / HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells. by P Cherepanov (2003)
10.1093/nar/gkl885 / Nucleic Acids Res / LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro. by P Cherepanov (2007)
10.1074/jbc.M411681200 / J Biol Chem / The interaction of LEDGF/p75 with integrase is lentivirus-specific and promotes DNA binding. by K Busschots (2005)
10.1128/JVI.78.17.9524-9537.2004 / J Virol / LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes. by M Llano (2004)
10.1074/jbc.M501378200 / J Biol Chem / Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication. by S Emiliani (2005)
10.1016/j.jmb.2006.04.073 / J Mol Biol / Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75. by M Llano (2006)
10.1074/jbc.M303594200 / J Biol Chem / LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells. by G Maertens (2003)
10.1038/nm1329 / Nat Med / A role for LEDGF/p75 in targeting HIV DNA integration. by A Ciuffi (2005)
10.1101/gad.1565107 / Genes Dev / LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. by MC Shun (2007)
10.1371/journal.pone.0001340 / PLoS ONE / Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting. by HM Marshall (2007)
10.1126/science.1132319 / Science / An essential role for LEDGF/p75 in HIV integration. by M Llano (2006)
10.1371/journal.ppat.0030047 / PLoS Pathog / Virus evolution reveals an exclusive role for LEDGF/p75 in chromosomal tethering of HIV. by A Hombrouck (2007)
10.1074/jbc.M406307200 / J Biol Chem / Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. by P Cherepanov (2004)
10.1038/nsmb937 / Nat Struct Mol Biol / Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75. by P Cherepanov (2005)
10.1242/jcs.02299 / J Cell Sci / Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering. by M Vanegas (2005)
10.1073/pnas.0506924102 / Proc Natl Acad Sci USA / Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75. by P Cherepanov (2005)
10.1016/S0021-9258(19)50138-7 / J Biol Chem / Structural implications of spectroscopic characterization of a putative zinc finger peptide from HIV-1 integrase. by CJ Burke (1992)
10.1016/j.virol.2006.08.011 / Virology / Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness. by S Rahman (2007)
10.1128/JVI.79.13.8208-8216.2005 / J Virol / Recombinant human immunodeficiency virus type 1 integrase exhibits a capacity for full-site integration in vitro that is comparable to that of purified preintegration complexes from virus-infected cells. by S Sinha (2005)
10.1074/jbc.M505367200 / J Biol Chem / Processing of viral DNA ends channels the HIV-1 integration reaction to concerted integration. by M Li (2005)
{'key': 'ref41', 'article-title': 'Functional and structural characterization of the integrase from the prototype foamy virus.', 'author': 'E Valkov', 'year': '2008', 'journal-title': 'Nucleic Acids Res'} / Nucleic Acids Res / Functional and structural characterization of the integrase from the prototype foamy virus. by E Valkov (2008)
10.1016/j.virol.2006.12.022 / Virology / LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration. by NK Raghavendra (2007)
10.1128/JVI.02322-06 / J Virol / Transcriptional coactivator LEDGF/p75 modulates human immunodeficiency virus type 1 integrase-mediated concerted integration. by KK Pandey (2007)
10.1016/S0065-3527(08)60309-7 / Adv Virus Res / In vivo analysis of retroviral integrase structure and function. by A Engelman (1999)
10.1016/j.sbi.2008.02.001 / Curr Opin Struct Biol / The structural biology of HIV assembly. by BK Ganser-Pornillos (2008)
10.1093/nar/27.10.2202 / Nucleic Acids Res / Activity of recombinant HIV-1 integrase on mini-HIV DNA. by P Cherepanov (1999)
10.1016/S0969-2126(03)00029-7 / Structure / The unfolding story of three-dimensional domain swapping. by F Rousseau (2003)
{'key': 'ref48', 'article-title': 'In vitro initial attachment of HIV-1 integrase to viral ends: control of the DNA specific interaction by the oligomerization state.', 'author': 'P Lesbats', 'year': '2008', 'journal-title': 'Nucleic Acids Res'} / Nucleic Acids Res / In vitro initial attachment of HIV-1 integrase to viral ends: control of the DNA specific interaction by the oligomerization state. by P Lesbats (2008)
10.1126/science.1088547 / Science / LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. by S Hacein-Bey-Abina (2003)
10.1089/hum.2006.17.960 / Hum Gene Ther / Modulating target site selection during human immunodeficiency virus DNA integration in vitro with an engineered tethering factor. by A Ciuffi (2006)
10.1093/nar/gkl052 / Nucleic Acids Res / A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. by F Turlure (2006)
10.1128/JVI.78.23.12735-12746.2004 / J Virol / Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. by R Lu (2004)
10.1016/S1097-2765(00)80326-3 / Mol Cell / Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. by E Mossessova (2000)
{'key': 'ref54', 'article-title': 'Recent changes to the MOSFLM package for processing film and image plate data.', 'volume': '26', 'author': 'AGW Leslie', 'year': '1992', 'journal-title': 'Joint CCP4+ESF-EAMCB Newsletter on Protein Crystallography'} / Joint CCP4+ESF-EAMCB Newsletter on Protein Crystallography / Recent changes to the MOSFLM package for processing film and image plate data. by AGW Leslie (1992)
{'key': 'ref55', 'first-page': '114', 'article-title': 'Data reduction.', 'author': 'PR Evans', 'year': '1993', 'journal-title': 'Proceedings of CCP4 Study Weekend, 1993, on Data Collection & Processing'} / Proceedings of CCP4 Study Weekend, 1993, on Data Collection & Processing / Data reduction. by PR Evans (1993)
10.1107/S0907444994003112 / Acta Crystallography D / The CCP 4 suite: programs for protein crystallography. (1994)
10.1107/S0021889897006766 / J Appl Cryst / MOLREP: an automated program for molecular replacement. by A Vagin (1997)
10.1107/S0907444996012255 / Acta Crystallogr D Biol Crystallogr / Refinement of macromolecular structures by the maximum-likelihood method. by GN Murshudov (1997)
10.1107/S0907444902016657 / Acta Crystallogr D Biol Crystallogr / PHENIX: building new software for automated crystallographic structure determination. by PD Adams (2002)
10.1107/S0907444904019158 / Acta Crystallogr D Biol Crystallogr / Coot: model-building tools for molecular graphics. by P Emsley (2004)
10.1107/S0021889807021206 / J Appl Cryst / Phaser crystallographic software. by AJ McCoy (2007)
10.1128/JVI.01953-06 / J Virol / Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein. by MC Shun (2007)

Dates

Type	When
Created	16 years, 7 months ago (Jan. 8, 2009, 5:46 p.m.)
Deposited	3 years, 11 months ago (Sept. 29, 2021, 12:49 p.m.)
Indexed	4 weeks ago (Aug. 7, 2025, 4:38 p.m.)
Issued	16 years, 7 months ago (Jan. 9, 2009)
Published	16 years, 7 months ago (Jan. 9, 2009)
Published Online	16 years, 7 months ago (Jan. 9, 2009)

Funders 0

None

BibTeX

@article{Hare_2009, title={A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75}, volume={5}, ISSN={1553-7374}, url={http://dx.doi.org/10.1371/journal.ppat.1000259}, DOI={10.1371/journal.ppat.1000259}, number={1}, journal={PLoS Pathogens}, publisher={Public Library of Science (PLoS)}, author={Hare, Stephen and Shun, Ming-Chieh and Gupta, Saumya Shree and Valkov, Eugene and Engelman, Alan and Cherepanov, Peter}, editor={Ross, Susan}, year={2009}, month=jan, pages={e1000259} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        7
      ]
    ],
    "date-time": "2025-08-07T20:38:51Z",
    "timestamp": 1754599131466
  },
  "reference-count": 62,
  "publisher": "Public Library of Science (PLoS)",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2009,
            1,
            9
          ]
        ],
        "date-time": "2009-01-09T00:00:00Z",
        "timestamp": 1231459200000
      },
      "content-version": "unspecified",
      "delay-in-days": 0,
      "URL": "http://creativecommons.org/licenses/by/4.0/"
    }
  ],
  "content-domain": {
    "domain": [
      "www.plospathogens.org"
    ],
    "crossmark-restriction": false
  },
  "DOI": "10.1371/journal.ppat.1000259",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        1,
        8
      ]
    ],
    "date-time": "2009-01-08T22:46:39Z",
    "timestamp": 1231454799000
  },
  "page": "e1000259",
  "update-policy": "http://dx.doi.org/10.1371/journal.ppat.corrections_policy",
  "source": "Crossref",
  "is-referenced-by-count": 132,
  "title": "A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75",
  "prefix": "10.1371",
  "volume": "5",
  "author": [
    {
      "given": "Stephen",
      "family": "Hare",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Ming-Chieh",
      "family": "Shun",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Saumya Shree",
      "family": "Gupta",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Eugene",
      "family": "Valkov",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Alan",
      "family": "Engelman",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Peter",
      "family": "Cherepanov",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "340",
  "published-online": {
    "date-parts": [
      [
        2009,
        1,
        9
      ]
    ]
  },
  "reference": [
    {
      "key": "ref1",
      "doi-asserted-by": "crossref",
      "first-page": "147",
      "DOI": "10.1016/S0065-2660(05)55005-3",
      "article-title": "Retroviral DNA integration\u2013mechanism and consequences.",
      "volume": "55",
      "author": "MK Lewinski",
      "year": "2005",
      "journal-title": "Adv Genet"
    },
    {
      "key": "ref2",
      "first-page": "613",
      "article-title": "Retroviral DNA Integration.",
      "author": "R Craigie",
      "year": "2002"
    },
    {
      "key": "ref3",
      "doi-asserted-by": "crossref",
      "first-page": "3428",
      "DOI": "10.1073/pnas.90.8.3428",
      "article-title": "Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding.",
      "volume": "90",
      "author": "FD Bushman",
      "year": "1993",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "ref4",
      "doi-asserted-by": "crossref",
      "first-page": "3269",
      "DOI": "10.1002/j.1460-2075.1993.tb05996.x",
      "article-title": "Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex.",
      "volume": "12",
      "author": "A Engelman",
      "year": "1993",
      "journal-title": "EMBO J"
    },
    {
      "key": "ref5",
      "doi-asserted-by": "crossref",
      "first-page": "6361",
      "DOI": "10.1128/JVI.66.11.6361-6369.1992",
      "article-title": "Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro.",
      "volume": "66",
      "author": "A Engelman",
      "year": "1992",
      "journal-title": "J Virol"
    },
    {
      "key": "ref6",
      "doi-asserted-by": "crossref",
      "first-page": "1981",
      "DOI": "10.1126/science.7801124",
      "article-title": "Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases.",
      "volume": "266",
      "author": "F Dyda",
      "year": "1994",
      "journal-title": "Science"
    },
    {
      "key": "ref7",
      "doi-asserted-by": "crossref",
      "first-page": "9150",
      "DOI": "10.1073/pnas.95.16.9150",
      "article-title": "Three new structures of the core domain of HIV-1 integrase: an active site that binds magnesium.",
      "volume": "95",
      "author": "Y Goldgur",
      "year": "1998",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "ref8",
      "doi-asserted-by": "crossref",
      "first-page": "5",
      "DOI": "10.1016/j.molcel.2006.03.013",
      "article-title": "Making and breaking nucleic acids: two-Mg2+\u2212ion catalysis and substrate specificity.",
      "volume": "22",
      "author": "W Yang",
      "year": "2006",
      "journal-title": "Mol Cell"
    },
    {
      "key": "ref9",
      "doi-asserted-by": "crossref",
      "first-page": "567",
      "DOI": "10.1038/nsb0797-567",
      "article-title": "Solution structure of the N-terminal zinc binding domain of HIV-1 integrase.",
      "volume": "4",
      "author": "M Cai",
      "year": "1997",
      "journal-title": "Nat Struct Biol"
    },
    {
      "key": "ref10",
      "doi-asserted-by": "crossref",
      "first-page": "739",
      "DOI": "10.1016/S0960-9822(06)00332-0",
      "article-title": "The solution structure of the amino-terminal HHCC domain of HIV-2 integrase: a three-helix bundle stabilized by zinc.",
      "volume": "7",
      "author": "AP Eijkelenboom",
      "year": "1997",
      "journal-title": "Curr Biol"
    },
    {
      "key": "ref11",
      "doi-asserted-by": "crossref",
      "first-page": "807",
      "DOI": "10.1038/nsb0995-807",
      "article-title": "The DNA-binding domain of HIV-1 integrase has an SH3-like fold.",
      "volume": "2",
      "author": "AP Eijkelenboom",
      "year": "1995",
      "journal-title": "Nat Struct Biol"
    },
    {
      "key": "ref12",
      "doi-asserted-by": "crossref",
      "first-page": "9826",
      "DOI": "10.1021/bi00031a002",
      "article-title": "Solution structure of the DNA binding domain of HIV-1 integrase.",
      "volume": "34",
      "author": "PJ Lodi",
      "year": "1995",
      "journal-title": "Biochemistry (Mosc)"
    },
    {
      "key": "ref13",
      "doi-asserted-by": "crossref",
      "first-page": "1323",
      "DOI": "10.1074/jbc.C200550200",
      "article-title": "Functional oligomeric state of avian sarcoma virus integrase.",
      "volume": "278",
      "author": "KK Bao",
      "year": "2003",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref14",
      "doi-asserted-by": "crossref",
      "first-page": "977",
      "DOI": "10.1093/nar/gki241",
      "article-title": "HIV-1 integrase crosslinked oligomers are active in vitro.",
      "volume": "33",
      "author": "A Faure",
      "year": "2005",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref15",
      "doi-asserted-by": "crossref",
      "first-page": "1295",
      "DOI": "10.1038/sj.emboj.7601005",
      "article-title": "Retroviral DNA integration: reaction pathway and critical intermediates.",
      "volume": "25",
      "author": "M Li",
      "year": "2006",
      "journal-title": "EMBO J"
    },
    {
      "key": "ref16",
      "doi-asserted-by": "crossref",
      "first-page": "7333",
      "DOI": "10.1093/emboj/20.24.7333",
      "article-title": "Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein.",
      "volume": "20",
      "author": "JY Wang",
      "year": "2001",
      "journal-title": "EMBO J"
    },
    {
      "key": "ref17",
      "doi-asserted-by": "crossref",
      "first-page": "e1000046",
      "DOI": "10.1371/journal.ppat.1000046",
      "article-title": "The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication.",
      "volume": "4",
      "author": "A Engelman",
      "year": "2008",
      "journal-title": "PLoS Pathog"
    },
    {
      "key": "ref18",
      "doi-asserted-by": "crossref",
      "first-page": "36",
      "DOI": "10.1016/j.ccr.2008.05.003",
      "article-title": "Menin critically links MLL proteins with LEDGF on cancer-associated target genes.",
      "volume": "14",
      "author": "A Yokoyama",
      "year": "2008",
      "journal-title": "Cancer Cell"
    },
    {
      "key": "ref19",
      "doi-asserted-by": "crossref",
      "first-page": "943",
      "DOI": "10.1006/bbrc.2001.4887",
      "article-title": "LEDGF binds to heat shock and stress-related element to activate the expression of stress-related genes.",
      "volume": "283",
      "author": "DP Singh",
      "year": "2001",
      "journal-title": "Biochem Biophys Res Commun"
    },
    {
      "key": "ref20",
      "doi-asserted-by": "crossref",
      "first-page": "915",
      "DOI": "10.1038/sj.cdd.4401063",
      "article-title": "Caspase cleavage of the nuclear autoantigen LEDGF/p75 abrogates its pro-survival function: implications for autoimmunity in atopic disorders.",
      "volume": "9",
      "author": "X Wu",
      "year": "2002",
      "journal-title": "Cell Death Differ"
    },
    {
      "key": "ref21",
      "doi-asserted-by": "crossref",
      "first-page": "372",
      "DOI": "10.1074/jbc.M209278200",
      "article-title": "HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells.",
      "volume": "278",
      "author": "P Cherepanov",
      "year": "2003",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref22",
      "doi-asserted-by": "crossref",
      "first-page": "113",
      "DOI": "10.1093/nar/gkl885",
      "article-title": "LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro.",
      "volume": "35",
      "author": "P Cherepanov",
      "year": "2007",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref23",
      "doi-asserted-by": "crossref",
      "first-page": "17841",
      "DOI": "10.1074/jbc.M411681200",
      "article-title": "The interaction of LEDGF/p75 with integrase is lentivirus-specific and promotes DNA binding.",
      "volume": "280",
      "author": "K Busschots",
      "year": "2005",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref24",
      "doi-asserted-by": "crossref",
      "first-page": "9524",
      "DOI": "10.1128/JVI.78.17.9524-9537.2004",
      "article-title": "LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes.",
      "volume": "78",
      "author": "M Llano",
      "year": "2004",
      "journal-title": "J Virol"
    },
    {
      "key": "ref25",
      "doi-asserted-by": "crossref",
      "first-page": "25517",
      "DOI": "10.1074/jbc.M501378200",
      "article-title": "Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication.",
      "volume": "280",
      "author": "S Emiliani",
      "year": "2005",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref26",
      "doi-asserted-by": "crossref",
      "first-page": "760",
      "DOI": "10.1016/j.jmb.2006.04.073",
      "article-title": "Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75.",
      "volume": "360",
      "author": "M Llano",
      "year": "2006",
      "journal-title": "J Mol Biol"
    },
    {
      "key": "ref27",
      "doi-asserted-by": "crossref",
      "first-page": "33528",
      "DOI": "10.1074/jbc.M303594200",
      "article-title": "LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells.",
      "volume": "278",
      "author": "G Maertens",
      "year": "2003",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref28",
      "doi-asserted-by": "crossref",
      "first-page": "1287",
      "DOI": "10.1038/nm1329",
      "article-title": "A role for LEDGF/p75 in targeting HIV DNA integration.",
      "volume": "11",
      "author": "A Ciuffi",
      "year": "2005",
      "journal-title": "Nat Med"
    },
    {
      "key": "ref29",
      "doi-asserted-by": "crossref",
      "first-page": "1767",
      "DOI": "10.1101/gad.1565107",
      "article-title": "LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration.",
      "volume": "21",
      "author": "MC Shun",
      "year": "2007",
      "journal-title": "Genes Dev"
    },
    {
      "key": "ref30",
      "doi-asserted-by": "crossref",
      "first-page": "e1340",
      "DOI": "10.1371/journal.pone.0001340",
      "article-title": "Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting.",
      "volume": "2",
      "author": "HM Marshall",
      "year": "2007",
      "journal-title": "PLoS ONE"
    },
    {
      "key": "ref31",
      "doi-asserted-by": "crossref",
      "first-page": "461",
      "DOI": "10.1126/science.1132319",
      "article-title": "An essential role for LEDGF/p75 in HIV integration.",
      "volume": "314",
      "author": "M Llano",
      "year": "2006",
      "journal-title": "Science"
    },
    {
      "key": "ref32",
      "doi-asserted-by": "crossref",
      "first-page": "e47",
      "DOI": "10.1371/journal.ppat.0030047",
      "article-title": "Virus evolution reveals an exclusive role for LEDGF/p75 in chromosomal tethering of HIV.",
      "volume": "3",
      "author": "A Hombrouck",
      "year": "2007",
      "journal-title": "PLoS Pathog"
    },
    {
      "key": "ref33",
      "doi-asserted-by": "crossref",
      "first-page": "48883",
      "DOI": "10.1074/jbc.M406307200",
      "article-title": "Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase.",
      "volume": "279",
      "author": "P Cherepanov",
      "year": "2004",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref34",
      "doi-asserted-by": "crossref",
      "first-page": "526",
      "DOI": "10.1038/nsmb937",
      "article-title": "Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75.",
      "volume": "12",
      "author": "P Cherepanov",
      "year": "2005",
      "journal-title": "Nat Struct Mol Biol"
    },
    {
      "key": "ref35",
      "doi-asserted-by": "crossref",
      "first-page": "1733",
      "DOI": "10.1242/jcs.02299",
      "article-title": "Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering.",
      "volume": "118",
      "author": "M Vanegas",
      "year": "2005",
      "journal-title": "J Cell Sci"
    },
    {
      "key": "ref36",
      "doi-asserted-by": "crossref",
      "first-page": "17308",
      "DOI": "10.1073/pnas.0506924102",
      "article-title": "Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75.",
      "volume": "102",
      "author": "P Cherepanov",
      "year": "2005",
      "journal-title": "Proc Natl Acad Sci USA"
    },
    {
      "key": "ref37",
      "doi-asserted-by": "crossref",
      "first-page": "9639",
      "DOI": "10.1016/S0021-9258(19)50138-7",
      "article-title": "Structural implications of spectroscopic characterization of a putative zinc finger peptide from HIV-1 integrase.",
      "volume": "267",
      "author": "CJ Burke",
      "year": "1992",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref38",
      "doi-asserted-by": "crossref",
      "first-page": "79",
      "DOI": "10.1016/j.virol.2006.08.011",
      "article-title": "Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness.",
      "volume": "357",
      "author": "S Rahman",
      "year": "2007",
      "journal-title": "Virology"
    },
    {
      "key": "ref39",
      "doi-asserted-by": "crossref",
      "first-page": "8208",
      "DOI": "10.1128/JVI.79.13.8208-8216.2005",
      "article-title": "Recombinant human immunodeficiency virus type 1 integrase exhibits a capacity for full-site integration in vitro that is comparable to that of purified preintegration complexes from virus-infected cells.",
      "volume": "79",
      "author": "S Sinha",
      "year": "2005",
      "journal-title": "J Virol"
    },
    {
      "key": "ref40",
      "doi-asserted-by": "crossref",
      "first-page": "29334",
      "DOI": "10.1074/jbc.M505367200",
      "article-title": "Processing of viral DNA ends channels the HIV-1 integration reaction to concerted integration.",
      "volume": "280",
      "author": "M Li",
      "year": "2005",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref41",
      "article-title": "Functional and structural characterization of the integrase from the prototype foamy virus.",
      "author": "E Valkov",
      "year": "2008",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref42",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/j.virol.2006.12.022",
      "article-title": "LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration.",
      "volume": "360",
      "author": "NK Raghavendra",
      "year": "2007",
      "journal-title": "Virology"
    },
    {
      "key": "ref43",
      "doi-asserted-by": "crossref",
      "first-page": "3969",
      "DOI": "10.1128/JVI.02322-06",
      "article-title": "Transcriptional coactivator LEDGF/p75 modulates human immunodeficiency virus type 1 integrase-mediated concerted integration.",
      "volume": "81",
      "author": "KK Pandey",
      "year": "2007",
      "journal-title": "J Virol"
    },
    {
      "key": "ref44",
      "doi-asserted-by": "crossref",
      "first-page": "411",
      "DOI": "10.1016/S0065-3527(08)60309-7",
      "article-title": "In vivo analysis of retroviral integrase structure and function.",
      "volume": "52",
      "author": "A Engelman",
      "year": "1999",
      "journal-title": "Adv Virus Res"
    },
    {
      "key": "ref45",
      "doi-asserted-by": "crossref",
      "first-page": "203",
      "DOI": "10.1016/j.sbi.2008.02.001",
      "article-title": "The structural biology of HIV assembly.",
      "volume": "18",
      "author": "BK Ganser-Pornillos",
      "year": "2008",
      "journal-title": "Curr Opin Struct Biol"
    },
    {
      "key": "ref46",
      "doi-asserted-by": "crossref",
      "first-page": "2202",
      "DOI": "10.1093/nar/27.10.2202",
      "article-title": "Activity of recombinant HIV-1 integrase on mini-HIV DNA.",
      "volume": "27",
      "author": "P Cherepanov",
      "year": "1999",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref47",
      "doi-asserted-by": "crossref",
      "first-page": "243",
      "DOI": "10.1016/S0969-2126(03)00029-7",
      "article-title": "The unfolding story of three-dimensional domain swapping.",
      "volume": "11",
      "author": "F Rousseau",
      "year": "2003",
      "journal-title": "Structure"
    },
    {
      "key": "ref48",
      "article-title": "In vitro initial attachment of HIV-1 integrase to viral ends: control of the DNA specific interaction by the oligomerization state.",
      "author": "P Lesbats",
      "year": "2008",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref49",
      "doi-asserted-by": "crossref",
      "first-page": "415",
      "DOI": "10.1126/science.1088547",
      "article-title": "LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1.",
      "volume": "302",
      "author": "S Hacein-Bey-Abina",
      "year": "2003",
      "journal-title": "Science"
    },
    {
      "key": "ref50",
      "doi-asserted-by": "crossref",
      "first-page": "960",
      "DOI": "10.1089/hum.2006.17.960",
      "article-title": "Modulating target site selection during human immunodeficiency virus DNA integration in vitro with an engineered tethering factor.",
      "volume": "17",
      "author": "A Ciuffi",
      "year": "2006",
      "journal-title": "Hum Gene Ther"
    },
    {
      "key": "ref51",
      "doi-asserted-by": "crossref",
      "first-page": "1653",
      "DOI": "10.1093/nar/gkl052",
      "article-title": "A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo.",
      "volume": "34",
      "author": "F Turlure",
      "year": "2006",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref52",
      "doi-asserted-by": "crossref",
      "first-page": "12735",
      "DOI": "10.1128/JVI.78.23.12735-12746.2004",
      "article-title": "Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication.",
      "volume": "78",
      "author": "R Lu",
      "year": "2004",
      "journal-title": "J Virol"
    },
    {
      "key": "ref53",
      "doi-asserted-by": "crossref",
      "first-page": "865",
      "DOI": "10.1016/S1097-2765(00)80326-3",
      "article-title": "Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast.",
      "volume": "5",
      "author": "E Mossessova",
      "year": "2000",
      "journal-title": "Mol Cell"
    },
    {
      "key": "ref54",
      "article-title": "Recent changes to the MOSFLM package for processing film and image plate data.",
      "volume": "26",
      "author": "AGW Leslie",
      "year": "1992",
      "journal-title": "Joint CCP4+ESF-EAMCB Newsletter on Protein Crystallography"
    },
    {
      "key": "ref55",
      "first-page": "114",
      "article-title": "Data reduction.",
      "author": "PR Evans",
      "year": "1993",
      "journal-title": "Proceedings of CCP4 Study Weekend, 1993, on Data Collection & Processing"
    },
    {
      "key": "ref56",
      "doi-asserted-by": "crossref",
      "first-page": "760",
      "DOI": "10.1107/S0907444994003112",
      "article-title": "The CCP 4 suite: programs for protein crystallography.",
      "volume": "50",
      "year": "1994",
      "journal-title": "Acta Crystallography D"
    },
    {
      "key": "ref57",
      "doi-asserted-by": "crossref",
      "first-page": "1022",
      "DOI": "10.1107/S0021889897006766",
      "article-title": "MOLREP: an automated program for molecular replacement.",
      "volume": "30",
      "author": "A Vagin",
      "year": "1997",
      "journal-title": "J Appl Cryst"
    },
    {
      "key": "ref58",
      "doi-asserted-by": "crossref",
      "first-page": "240",
      "DOI": "10.1107/S0907444996012255",
      "article-title": "Refinement of macromolecular structures by the maximum-likelihood method.",
      "volume": "53",
      "author": "GN Murshudov",
      "year": "1997",
      "journal-title": "Acta Crystallogr D Biol Crystallogr"
    },
    {
      "key": "ref59",
      "doi-asserted-by": "crossref",
      "first-page": "1948",
      "DOI": "10.1107/S0907444902016657",
      "article-title": "PHENIX: building new software for automated crystallographic structure determination.",
      "volume": "58",
      "author": "PD Adams",
      "year": "2002",
      "journal-title": "Acta Crystallogr D Biol Crystallogr"
    },
    {
      "key": "ref60",
      "doi-asserted-by": "crossref",
      "first-page": "2126",
      "DOI": "10.1107/S0907444904019158",
      "article-title": "Coot: model-building tools for molecular graphics.",
      "volume": "60",
      "author": "P Emsley",
      "year": "2004",
      "journal-title": "Acta Crystallogr D Biol Crystallogr"
    },
    {
      "key": "ref61",
      "doi-asserted-by": "crossref",
      "first-page": "658",
      "DOI": "10.1107/S0021889807021206",
      "article-title": "Phaser crystallographic software.",
      "volume": "40",
      "author": "AJ McCoy",
      "year": "2007",
      "journal-title": "J Appl Cryst"
    },
    {
      "key": "ref62",
      "doi-asserted-by": "crossref",
      "first-page": "166",
      "DOI": "10.1128/JVI.01953-06",
      "article-title": "Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein.",
      "volume": "81",
      "author": "MC Shun",
      "year": "2007",
      "journal-title": "J Virol"
    }
  ],
  "container-title": "PLoS Pathogens",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "http://dx.plos.org/10.1371/journal.ppat.1000259",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        9,
        29
      ]
    ],
    "date-time": "2021-09-29T16:49:35Z",
    "timestamp": 1632934175000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://dx.plos.org/10.1371/journal.ppat.1000259"
    }
  },
  "subtitle": [],
  "editor": [
    {
      "given": "Susan",
      "family": "Ross",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2009,
        1,
        9
      ]
    ]
  },
  "references-count": 62,
  "journal-issue": {
    "issue": "1",
    "published-online": {
      "date-parts": [
        [
          2009,
          1,
          9
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1371/journal.ppat.1000259",
  "relation": {},
  "ISSN": [
    "1553-7374"
  ],
  "subject": [],
  "container-title-short": "PLoS Pathog",
  "published": {
    "date-parts": [
      [
        2009,
        1,
        9
      ]
    ]
  }
}