DOI: 10.1371/journal.pone.0098056. Identification of T. gondii Myosin Light Chain-1 as a Direct Target of TachypleginA-2, a Small-Molecule Inhibitor of Parasite Motility and Invasion

Identification of T. gondii Myosin Light Chain-1 as a Direct Target of TachypleginA-2, a Small-Molecule Inhibitor of Parasite Motility and Invasion

10.1371/journal.pone.0098056

Crossref journal-article

Public Library of Science (PLoS)

PLoS ONE (340)

Bibliography

Leung, J. M., Tran, F., Pathak, R. B., Poupart, S., Heaslip, A. T., Ballif, B. A., Westwood, N. J., & Ward, G. E. (2014). Identification of T. gondii Myosin Light Chain-1 as a Direct Target of TachypleginA-2, a Small-Molecule Inhibitor of Parasite Motility and Invasion. PLoS ONE, 9(6), e98056.

Authors 8

Jacqueline M. Leung (first)
Fanny Tran (additional)
Ravindra B. Pathak (additional)
Séverine Poupart (additional)
Aoife T. Heaslip (additional)
Bryan A. Ballif (additional)
Nicholas J. Westwood (additional)
Gary E. Ward (additional)

References 51 Referenced 18

10.1126/science.1094717 / Science / Intracellular parasite invasion strategies by LD Sibley (2004)
10.1093/emboj/21.9.2149 / EMBO J / <italic>Toxoplasma gondii</italic> myosin A and its light chain: a fast, single-headed, plus-end-directed motor by A Herm-Gotz (2002)
10.1371/journal.ppat.1002222 / PLoS Pathog / Quantitative in vivo analyses reveal calcium-dependent phosphorylation sites and identifies a novel component of the <italic>Toxoplasma</italic> invasion motor complex by T Nebl (2011)
10.1016/j.chom.2010.09.002 / Cell Host Microbe / Functional dissection of the apicomplexan glideosome molecular architecture by K Frenal (2010)
10.1083/jcb.200311137 / J Cell Biol / Identification of the membrane receptor of a class XIV myosin in <italic>Toxoplasma gondii</italic> by E Gaskins (2004)
10.1111/j.1462-5822.2010.01514.x / Cell Microbiol / A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of <italic>Toxoplasma gondii</italic> by BR Anderson-White (2010)
10.1016/S0166-6851(01)00289-4 / Mol Biochem Parasitol / Characterization of the subpellicular network, a filamentous membrane skeletal component in the parasite <italic>Toxoplasma gondii</italic> by T Mann (2001)
{'key': 'ref8', 'first-page': '101', 'article-title': '[Freeze fracture study of <italic>Toxoplasma</italic> and <italic>Sarcocystis</italic> infective stages (author's transl)]', 'volume': '54', 'author': 'E Porchet', 'year': '1977', 'journal-title': 'Z Parasitenkd'} / Z Parasitenkd / [Freeze fracture study of <italic>Toxoplasma</italic> and <italic>Sarcocystis</italic> infective stages (author's transl)] by E Porchet (1977)
10.1126/science.1074553 / Science / Role of <italic>Toxoplasma gondii</italic> myosin A in powering parasite gliding and host cell invasion by M Meissner (2002)
Andenmatten N, Egarter S, Jackson AJ, Jullien N, Herman JP, <etal>et al</etal>.. (2012) Conditional genome engineering in <italic>Toxoplasma gondii</italic> uncovers alternative invasion mechanisms. Nat Methods: 125–127. (10.1038/nmeth.2301)
10.1007/BF00121066 / J Muscle Res Cell Motil / Role of myosin light chains by KM Trybus (1994)
10.1016/S0021-9258(17)31894-X / J Biol Chem / Regulation of expressed truncated smooth muscle myosins. Role of the essential light chain and tail length by KM Trybus (1994)
{'key': 'ref13', 'first-page': '120S', 'article-title': 'Role of skeletal and smooth muscle myosin light chains', 'volume': '68', 'author': 'S Lowey', 'year': '1995', 'journal-title': 'Biophys J'} / Biophys J / Role of skeletal and smooth muscle myosin light chains by S Lowey (1995)
10.1111/j.1600-0854.2010.01148.x / Traffic / Unusual anchor of a motor complex (MyoD-MLC2) to the plasma membrane of <italic>Toxoplasma gondii</italic> by V Polonais (2011)
10.1007/s10822-011-9420-6 / J Comput Aided Mol Des / Rapid discovery of inhibitors of <italic>Toxoplasma gondii</italic> using hybrid structure-based computational approach by S Kortagere (2011)
10.1073/pnas.0307769101 / Proc Natl Acad Sci U S A / A small-molecule approach to studying invasive mechanisms of <italic>Toxoplasma gondii</italic> by KL Carey (2004)
10.1371/journal.ppat.1000720 / PLoS Pathog / A small-molecule inhibitor of <italic>T. gondii</italic> motility induces the posttranslational modification of myosin light chain-1 and inhibits myosin motor activity by AT Heaslip (2010)
10.1128/EC.00357-08 / Eukaryot Cell / Efficient gene replacements in <italic>Toxoplasma gondii</italic> strains deficient for nonhomologous end joining by BA Fox (2009)
10.1128/EC.00358-08 / Eukaryot Cell / Tagging of endogenous genes in a <italic>Toxoplasma gondii</italic> strain lacking Ku80 by MH Huynh (2009)
{'key': 'ref20', 'first-page': '27', 'article-title': 'Molecular tools for genetic dissection of the protozoan parasite <italic>Toxoplasma gondii</italic>', 'author': 'DS Roos', 'year': '1994', 'journal-title': 'Methods in Cell Biology'} / Methods in Cell Biology / Molecular tools for genetic dissection of the protozoan parasite <italic>Toxoplasma gondii</italic> by DS Roos (1994)
10.1002/cber.188101402192 / Berichte der deutschen chemischen Gesellschaft / Condensationen von Ketonen mit Aldehyden by L Claisen (1881)
10.1021/jm0002580 / J Med Chem / A conformational and structure-activity relationship study of cytotoxic 3,5-bis(arylidene)-4-piperidones and related N-acryloyl analogues by JR Dimmock (2001)
10.1002/cber.188101401306 / Berichte der deutschen chemischen Gesellschaft / Ueber die Einwirkung von Aceton auf Furfurol und auf Bittermandelöl bei Gegenwart von Alkalilauge by JG Schmidt (1881)
10.1016/j.tet.2008.07.063 / Tetrahedron / The synthesis of highly functionalised pyridines using Ghosez-type reactions of dihydropyrazoles by F Catti (2008)
10.1073/pnas.1105622108 / Proc Natl Acad Sci U S A / Chemical genetic screen identifies <italic>Toxoplasma</italic> DJ-1 as a regulator of parasite secretion, attachment, and invasion by CI Hall (2011)
10.1371/journal.pone.0008143 / PLoS One / 4-bromophenacyl bromide specifically inhibits rhoptry secretion during <italic>Toxoplasma</italic> invasion by S Ravindran (2009)
10.1002/(SICI)1097-0169(1997)37:3<253::AID-CM7>3.0.CO;2-7 / Cell Motil Cytoskeleton / Actin in the parasite <italic>Toxoplasma gondii</italic> is encoded by a single copy gene, ACT1 and exists primarily in a globular form by JM Dobrowolski (1997)
10.1038/nprot.2009.21 / Nat Protoc / Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics by PJ Boersema (2009)
10.1021/ac0348625 / Anal Chem / Stable-isotope dimethyl labeling for quantitative proteomics by JL Hsu (2003)
10.1074/mcp.M500339-MCP200 / Mol Cell Proteomics / Optimization and use of peptide mass measurement accuracy in shotgun proteomics by W Haas (2006)
10.1002/emmm.201302466 / EMBO Mol Med / Disruption of SMIM1 causes the Vel- blood type by BA Ballif (2013)
10.1016/S0040-4039(03)00247-8 / Tetrahedron Letters / Solid-phase aldol condensations mediated by zinc acetate and 2,2′-bipyridine under weakly basic conditions by U Sensfuss (2003)
10.1016/j.tetlet.2005.10.062 / Tetrahedron Letters / A mild and highly convenient chemoselective alkylation of thiols using Cs2CO3–TBAI by RN Salvatore (2005)
10.1091/mbc.01-06-0309 / Mol Biol Cell / Daughter cell assembly in the protozoan parasite <italic>Toxoplasma gondii</italic> by K Hu (2002)
10.1038/nprot.2006.405 / Nat Protoc / Fusion PCR and gene targeting in Aspergillus nidulans by E Szewczyk (2006)
10.1016/0378-1119(95)00548-K / Gene / Stable DNA transformation of <italic>Toxoplasma gondii</italic> using phleomycin selection by M Messina (1995)
10.1073/pnas.0803862105 / Proc Natl Acad Sci U S A / <italic>Toxoplasma gondii</italic> Tic20 is essential for apicoplast protein import by GG van Dooren (2008)
10.1093/nar/gks1113 / Nucleic Acids Res / EuPathDB: the eukaryotic pathogen database by C Aurrecoechea (2013)
10.1093/nar/gkm981 / Nucleic Acids Res / ToxoDB: an integrated Toxoplasma gondii database resource by B Gajria (2008)
10.1371/journal.pone.0085763 / PLoS One / Disruption of TgPHIL1 Alters Specific Parameters of Toxoplasma gondii Motility Measured in a Quantitative, Three-Dimensional Live Motility Assay by JM Leung (2014)
10.1016/0010-8545(90)85016-L / Coordination Chemistry Reviews / Hard and soft acids and bases—the evolution of a chemical concept by RG Pearson (1990)
10.1038/nprot.2006.427 / Nat Protoc / A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC) by SE Ong (2006)
10.1371/journal.ppat.0020084 / PLoS Pathog / <italic>Toxoplasma</italic> MIC2 is a major determinant of invasion and virulence by MH Huynh (2006)
10.1097/00001813-200503000-00005 / Anticancer Drugs / EF24, a novel synthetic curcumin analog, induces apoptosis in cancer cells via a redox-dependent mechanism by BK Adams (2005)
10.1016/j.bmcl.2009.10.023 / Bioorg Med Chem Lett / Curcumin analog cytotoxicity against breast cancer cells: exploitation of a redox-dependent mechanism by A Sun (2009)
10.1016/j.ejmech.2008.03.015 / Eur J Med Chem / The cytotoxic properties and preferential toxicity to tumour cells displayed by some 2,4-bis(benzylidene)-8-methyl-8-azabicyclo[3.2.1] octan-3-ones and 3,5-bis(benzylidene)-1-methyl-4-piperidones by HN Pati (2009)
10.2174/138955707779802642 / Mini Rev Med Chem / Cytotoxic thiol alkylators by HN Pati (2007)
10.1016/0003-2697(89)90045-6 / Anal Biochem / 1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one hydrobromide, a sulfhydryl-specific compound which reacts irreversibly with protein thiols but reversibly with small molecular weight thiols by B Mutus (1989)
{'key': 'ref49', 'first-page': '2053', 'article-title': 'Reactivity of some α, β-unsaturated ketones towards sulphydryl compounds and their antifungal activity', 'volume': '1964', 'author': 'G Baluja', 'year': '1964', 'journal-title': 'Chem Ind'} / Chem Ind / Reactivity of some α, β-unsaturated ketones towards sulphydryl compounds and their antifungal activity by G Baluja (1964)
10.1016/j.jmb.2007.06.016 / J Mol Biol / The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery by J Bosch (2007)
10.1016/j.chom.2011.09.004 / Cell Host Microbe / The phosphoproteomes of <italic>Plasmodium falciparum</italic> and <italic>Toxoplasma gondii</italic> reveal unusual adaptations within and beyond the parasites' boundaries by M Treeck (2011)

Dates

Type	When
Created	11 years, 2 months ago (June 3, 2014, 4:23 p.m.)
Deposited	3 years, 4 months ago (April 5, 2022, 5:10 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 3:13 p.m.)
Issued	11 years, 3 months ago (June 3, 2014)
Published	11 years, 3 months ago (June 3, 2014)
Published Online	11 years, 3 months ago (June 3, 2014)

Funders 0

None

BibTeX

@article{Leung_2014, title={Identification of T. gondii Myosin Light Chain-1 as a Direct Target of TachypleginA-2, a Small-Molecule Inhibitor of Parasite Motility and Invasion}, volume={9}, ISSN={1932-6203}, url={http://dx.doi.org/10.1371/journal.pone.0098056}, DOI={10.1371/journal.pone.0098056}, number={6}, journal={PLoS ONE}, publisher={Public Library of Science (PLoS)}, author={Leung, Jacqueline M. and Tran, Fanny and Pathak, Ravindra B. and Poupart, Séverine and Heaslip, Aoife T. and Ballif, Bryan A. and Westwood, Nicholas J. and Ward, Gary E.}, editor={Blader, Ira J.}, year={2014}, month=jun, pages={e98056} }

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      "article-title": "Intracellular parasite invasion strategies",
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      "article-title": "<italic>Toxoplasma gondii</italic> myosin A and its light chain: a fast, single-headed, plus-end-directed motor",
      "volume": "21",
      "author": "A Herm-Gotz",
      "year": "2002",
      "journal-title": "EMBO J"
    },
    {
      "key": "ref3",
      "doi-asserted-by": "crossref",
      "first-page": "e1002222",
      "DOI": "10.1371/journal.ppat.1002222",
      "article-title": "Quantitative in vivo analyses reveal calcium-dependent phosphorylation sites and identifies a novel component of the <italic>Toxoplasma</italic> invasion motor complex",
      "volume": "7",
      "author": "T Nebl",
      "year": "2011",
      "journal-title": "PLoS Pathog"
    },
    {
      "key": "ref4",
      "doi-asserted-by": "crossref",
      "first-page": "343",
      "DOI": "10.1016/j.chom.2010.09.002",
      "article-title": "Functional dissection of the apicomplexan glideosome molecular architecture",
      "volume": "8",
      "author": "K Frenal",
      "year": "2010",
      "journal-title": "Cell Host Microbe"
    },
    {
      "key": "ref5",
      "doi-asserted-by": "crossref",
      "first-page": "383",
      "DOI": "10.1083/jcb.200311137",
      "article-title": "Identification of the membrane receptor of a class XIV myosin in <italic>Toxoplasma gondii</italic>",
      "volume": "165",
      "author": "E Gaskins",
      "year": "2004",
      "journal-title": "J Cell Biol"
    },
    {
      "key": "ref6",
      "doi-asserted-by": "crossref",
      "first-page": "18",
      "DOI": "10.1111/j.1462-5822.2010.01514.x",
      "article-title": "A family of intermediate filament-like proteins is sequentially assembled into the cytoskeleton of <italic>Toxoplasma gondii</italic>",
      "volume": "13",
      "author": "BR Anderson-White",
      "year": "2010",
      "journal-title": "Cell Microbiol"
    },
    {
      "key": "ref7",
      "doi-asserted-by": "crossref",
      "first-page": "257",
      "DOI": "10.1016/S0166-6851(01)00289-4",
      "article-title": "Characterization of the subpellicular network, a filamentous membrane skeletal component in the parasite <italic>Toxoplasma gondii</italic>",
      "volume": "115",
      "author": "T Mann",
      "year": "2001",
      "journal-title": "Mol Biochem Parasitol"
    },
    {
      "key": "ref8",
      "first-page": "101",
      "article-title": "[Freeze fracture study of <italic>Toxoplasma</italic> and <italic>Sarcocystis</italic> infective stages (author&apos;s transl)]",
      "volume": "54",
      "author": "E Porchet",
      "year": "1977",
      "journal-title": "Z Parasitenkd"
    },
    {
      "key": "ref9",
      "doi-asserted-by": "crossref",
      "first-page": "837",
      "DOI": "10.1126/science.1074553",
      "article-title": "Role of <italic>Toxoplasma gondii</italic> myosin A in powering parasite gliding and host cell invasion",
      "volume": "298",
      "author": "M Meissner",
      "year": "2002",
      "journal-title": "Science"
    },
    {
      "key": "ref10",
      "doi-asserted-by": "crossref",
      "unstructured": "Andenmatten N, Egarter S, Jackson AJ, Jullien N, Herman JP, <etal>et al</etal>.. (2012) Conditional genome engineering in <italic>Toxoplasma gondii</italic> uncovers alternative invasion mechanisms. Nat Methods: 125\u2013127.",
      "DOI": "10.1038/nmeth.2301"
    },
    {
      "key": "ref11",
      "doi-asserted-by": "crossref",
      "first-page": "587",
      "DOI": "10.1007/BF00121066",
      "article-title": "Role of myosin light chains",
      "volume": "15",
      "author": "KM Trybus",
      "year": "1994",
      "journal-title": "J Muscle Res Cell Motil"
    },
    {
      "key": "ref12",
      "doi-asserted-by": "crossref",
      "first-page": "20819",
      "DOI": "10.1016/S0021-9258(17)31894-X",
      "article-title": "Regulation of expressed truncated smooth muscle myosins. Role of the essential light chain and tail length",
      "volume": "269",
      "author": "KM Trybus",
      "year": "1994",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "ref13",
      "first-page": "120S",
      "article-title": "Role of skeletal and smooth muscle myosin light chains",
      "volume": "68",
      "author": "S Lowey",
      "year": "1995",
      "journal-title": "Biophys J"
    },
    {
      "key": "ref14",
      "doi-asserted-by": "crossref",
      "first-page": "287",
      "DOI": "10.1111/j.1600-0854.2010.01148.x",
      "article-title": "Unusual anchor of a motor complex (MyoD-MLC2) to the plasma membrane of <italic>Toxoplasma gondii</italic>",
      "volume": "12",
      "author": "V Polonais",
      "year": "2011",
      "journal-title": "Traffic"
    },
    {
      "key": "ref15",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1007/s10822-011-9420-6",
      "article-title": "Rapid discovery of inhibitors of <italic>Toxoplasma gondii</italic> using hybrid structure-based computational approach",
      "volume": "25",
      "author": "S Kortagere",
      "year": "2011",
      "journal-title": "J Comput Aided Mol Des"
    },
    {
      "key": "ref16",
      "doi-asserted-by": "crossref",
      "first-page": "7433",
      "DOI": "10.1073/pnas.0307769101",
      "article-title": "A small-molecule approach to studying invasive mechanisms of <italic>Toxoplasma gondii</italic>",
      "volume": "101",
      "author": "KL Carey",
      "year": "2004",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref17",
      "doi-asserted-by": "crossref",
      "first-page": "e1000720",
      "DOI": "10.1371/journal.ppat.1000720",
      "article-title": "A small-molecule inhibitor of <italic>T. gondii</italic> motility induces the posttranslational modification of myosin light chain-1 and inhibits myosin motor activity",
      "volume": "6",
      "author": "AT Heaslip",
      "year": "2010",
      "journal-title": "PLoS Pathog"
    },
    {
      "key": "ref18",
      "doi-asserted-by": "crossref",
      "first-page": "520",
      "DOI": "10.1128/EC.00357-08",
      "article-title": "Efficient gene replacements in <italic>Toxoplasma gondii</italic> strains deficient for nonhomologous end joining",
      "volume": "8",
      "author": "BA Fox",
      "year": "2009",
      "journal-title": "Eukaryot Cell"
    },
    {
      "key": "ref19",
      "doi-asserted-by": "crossref",
      "first-page": "530",
      "DOI": "10.1128/EC.00358-08",
      "article-title": "Tagging of endogenous genes in a <italic>Toxoplasma gondii</italic> strain lacking Ku80",
      "volume": "8",
      "author": "MH Huynh",
      "year": "2009",
      "journal-title": "Eukaryot Cell"
    },
    {
      "key": "ref20",
      "first-page": "27",
      "article-title": "Molecular tools for genetic dissection of the protozoan parasite <italic>Toxoplasma gondii</italic>",
      "author": "DS Roos",
      "year": "1994",
      "journal-title": "Methods in Cell Biology"
    },
    {
      "key": "ref21",
      "doi-asserted-by": "crossref",
      "first-page": "2460",
      "DOI": "10.1002/cber.188101402192",
      "article-title": "Condensationen von Ketonen mit Aldehyden",
      "volume": "14",
      "author": "L Claisen",
      "year": "1881",
      "journal-title": "Berichte der deutschen chemischen Gesellschaft"
    },
    {
      "key": "ref22",
      "doi-asserted-by": "crossref",
      "first-page": "586",
      "DOI": "10.1021/jm0002580",
      "article-title": "A conformational and structure-activity relationship study of cytotoxic 3,5-bis(arylidene)-4-piperidones and related N-acryloyl analogues",
      "volume": "44",
      "author": "JR Dimmock",
      "year": "2001",
      "journal-title": "J Med Chem"
    },
    {
      "key": "ref23",
      "doi-asserted-by": "crossref",
      "first-page": "1459",
      "DOI": "10.1002/cber.188101401306",
      "article-title": "Ueber die Einwirkung von Aceton auf Furfurol und auf Bittermandel\u00f6l bei Gegenwart von Alkalilauge",
      "volume": "14",
      "author": "JG Schmidt",
      "year": "1881",
      "journal-title": "Berichte der deutschen chemischen Gesellschaft"
    },
    {
      "key": "ref24",
      "doi-asserted-by": "crossref",
      "first-page": "9561",
      "DOI": "10.1016/j.tet.2008.07.063",
      "article-title": "The synthesis of highly functionalised pyridines using Ghosez-type reactions of dihydropyrazoles",
      "volume": "64",
      "author": "F Catti",
      "year": "2008",
      "journal-title": "Tetrahedron"
    },
    {
      "key": "ref25",
      "doi-asserted-by": "crossref",
      "first-page": "10568",
      "DOI": "10.1073/pnas.1105622108",
      "article-title": "Chemical genetic screen identifies <italic>Toxoplasma</italic> DJ-1 as a regulator of parasite secretion, attachment, and invasion",
      "volume": "108",
      "author": "CI Hall",
      "year": "2011",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref26",
      "doi-asserted-by": "crossref",
      "first-page": "e8143",
      "DOI": "10.1371/journal.pone.0008143",
      "article-title": "4-bromophenacyl bromide specifically inhibits rhoptry secretion during <italic>Toxoplasma</italic> invasion",
      "volume": "4",
      "author": "S Ravindran",
      "year": "2009",
      "journal-title": "PLoS One"
    },
    {
      "key": "ref27",
      "doi-asserted-by": "crossref",
      "first-page": "253",
      "DOI": "10.1002/(SICI)1097-0169(1997)37:3<253::AID-CM7>3.0.CO;2-7",
      "article-title": "Actin in the parasite <italic>Toxoplasma gondii</italic> is encoded by a single copy gene, ACT1 and exists primarily in a globular form",
      "volume": "37",
      "author": "JM Dobrowolski",
      "year": "1997",
      "journal-title": "Cell Motil Cytoskeleton"
    },
    {
      "key": "ref28",
      "doi-asserted-by": "crossref",
      "first-page": "484",
      "DOI": "10.1038/nprot.2009.21",
      "article-title": "Multiplex peptide stable isotope dimethyl labeling for quantitative proteomics",
      "volume": "4",
      "author": "PJ Boersema",
      "year": "2009",
      "journal-title": "Nat Protoc"
    },
    {
      "key": "ref29",
      "doi-asserted-by": "crossref",
      "first-page": "6843",
      "DOI": "10.1021/ac0348625",
      "article-title": "Stable-isotope dimethyl labeling for quantitative proteomics",
      "volume": "75",
      "author": "JL Hsu",
      "year": "2003",
      "journal-title": "Anal Chem"
    },
    {
      "key": "ref30",
      "doi-asserted-by": "crossref",
      "first-page": "1326",
      "DOI": "10.1074/mcp.M500339-MCP200",
      "article-title": "Optimization and use of peptide mass measurement accuracy in shotgun proteomics",
      "volume": "5",
      "author": "W Haas",
      "year": "2006",
      "journal-title": "Mol Cell Proteomics"
    },
    {
      "key": "ref31",
      "doi-asserted-by": "crossref",
      "first-page": "751",
      "DOI": "10.1002/emmm.201302466",
      "article-title": "Disruption of SMIM1 causes the Vel- blood type",
      "volume": "5",
      "author": "BA Ballif",
      "year": "2013",
      "journal-title": "EMBO Mol Med"
    },
    {
      "key": "ref32",
      "doi-asserted-by": "crossref",
      "first-page": "2371",
      "DOI": "10.1016/S0040-4039(03)00247-8",
      "article-title": "Solid-phase aldol condensations mediated by zinc acetate and 2,2\u2032-bipyridine under weakly basic conditions",
      "volume": "44",
      "author": "U Sensfuss",
      "year": "2003",
      "journal-title": "Tetrahedron Letters"
    },
    {
      "key": "ref33",
      "doi-asserted-by": "crossref",
      "first-page": "8931",
      "DOI": "10.1016/j.tetlet.2005.10.062",
      "article-title": "A mild and highly convenient chemoselective alkylation of thiols using Cs2CO3\u2013TBAI",
      "volume": "46",
      "author": "RN Salvatore",
      "year": "2005",
      "journal-title": "Tetrahedron Letters"
    },
    {
      "key": "ref34",
      "doi-asserted-by": "crossref",
      "first-page": "593",
      "DOI": "10.1091/mbc.01-06-0309",
      "article-title": "Daughter cell assembly in the protozoan parasite <italic>Toxoplasma gondii</italic>",
      "volume": "13",
      "author": "K Hu",
      "year": "2002",
      "journal-title": "Mol Biol Cell"
    },
    {
      "key": "ref35",
      "doi-asserted-by": "crossref",
      "first-page": "3111",
      "DOI": "10.1038/nprot.2006.405",
      "article-title": "Fusion PCR and gene targeting in Aspergillus nidulans",
      "volume": "1",
      "author": "E Szewczyk",
      "year": "2006",
      "journal-title": "Nat Protoc"
    },
    {
      "key": "ref36",
      "doi-asserted-by": "crossref",
      "first-page": "213",
      "DOI": "10.1016/0378-1119(95)00548-K",
      "article-title": "Stable DNA transformation of <italic>Toxoplasma gondii</italic> using phleomycin selection",
      "volume": "165",
      "author": "M Messina",
      "year": "1995",
      "journal-title": "Gene"
    },
    {
      "key": "ref37",
      "doi-asserted-by": "crossref",
      "first-page": "13574",
      "DOI": "10.1073/pnas.0803862105",
      "article-title": "<italic>Toxoplasma gondii</italic> Tic20 is essential for apicoplast protein import",
      "volume": "105",
      "author": "GG van Dooren",
      "year": "2008",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "ref38",
      "doi-asserted-by": "crossref",
      "first-page": "D684",
      "DOI": "10.1093/nar/gks1113",
      "article-title": "EuPathDB: the eukaryotic pathogen database",
      "volume": "41",
      "author": "C Aurrecoechea",
      "year": "2013",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref39",
      "doi-asserted-by": "crossref",
      "first-page": "D553",
      "DOI": "10.1093/nar/gkm981",
      "article-title": "ToxoDB: an integrated Toxoplasma gondii database resource",
      "volume": "36",
      "author": "B Gajria",
      "year": "2008",
      "journal-title": "Nucleic Acids Res"
    },
    {
      "key": "ref40",
      "doi-asserted-by": "crossref",
      "first-page": "e85763",
      "DOI": "10.1371/journal.pone.0085763",
      "article-title": "Disruption of TgPHIL1 Alters Specific Parameters of Toxoplasma gondii Motility Measured in a Quantitative, Three-Dimensional Live Motility Assay",
      "volume": "9",
      "author": "JM Leung",
      "year": "2014",
      "journal-title": "PLoS One"
    },
    {
      "key": "ref41",
      "doi-asserted-by": "crossref",
      "first-page": "403",
      "DOI": "10.1016/0010-8545(90)85016-L",
      "article-title": "Hard and soft acids and bases\u2014the evolution of a chemical concept",
      "volume": "100",
      "author": "RG Pearson",
      "year": "1990",
      "journal-title": "Coordination Chemistry Reviews"
    },
    {
      "key": "ref42",
      "doi-asserted-by": "crossref",
      "first-page": "2650",
      "DOI": "10.1038/nprot.2006.427",
      "article-title": "A practical recipe for stable isotope labeling by amino acids in cell culture (SILAC)",
      "volume": "1",
      "author": "SE Ong",
      "year": "2006",
      "journal-title": "Nat Protoc"
    },
    {
      "key": "ref43",
      "doi-asserted-by": "crossref",
      "first-page": "e84",
      "DOI": "10.1371/journal.ppat.0020084",
      "article-title": "<italic>Toxoplasma</italic> MIC2 is a major determinant of invasion and virulence",
      "volume": "2",
      "author": "MH Huynh",
      "year": "2006",
      "journal-title": "PLoS Pathog"
    },
    {
      "key": "ref44",
      "doi-asserted-by": "crossref",
      "first-page": "263",
      "DOI": "10.1097/00001813-200503000-00005",
      "article-title": "EF24, a novel synthetic curcumin analog, induces apoptosis in cancer cells via a redox-dependent mechanism",
      "volume": "16",
      "author": "BK Adams",
      "year": "2005",
      "journal-title": "Anticancer Drugs"
    },
    {
      "key": "ref45",
      "doi-asserted-by": "crossref",
      "first-page": "6627",
      "DOI": "10.1016/j.bmcl.2009.10.023",
      "article-title": "Curcumin analog cytotoxicity against breast cancer cells: exploitation of a redox-dependent mechanism",
      "volume": "19",
      "author": "A Sun",
      "year": "2009",
      "journal-title": "Bioorg Med Chem Lett"
    },
    {
      "key": "ref46",
      "doi-asserted-by": "crossref",
      "first-page": "54",
      "DOI": "10.1016/j.ejmech.2008.03.015",
      "article-title": "The cytotoxic properties and preferential toxicity to tumour cells displayed by some 2,4-bis(benzylidene)-8-methyl-8-azabicyclo[3.2.1] octan-3-ones and 3,5-bis(benzylidene)-1-methyl-4-piperidones",
      "volume": "44",
      "author": "HN Pati",
      "year": "2009",
      "journal-title": "Eur J Med Chem"
    },
    {
      "key": "ref47",
      "doi-asserted-by": "crossref",
      "first-page": "131",
      "DOI": "10.2174/138955707779802642",
      "article-title": "Cytotoxic thiol alkylators",
      "volume": "7",
      "author": "HN Pati",
      "year": "2007",
      "journal-title": "Mini Rev Med Chem"
    },
    {
      "key": "ref48",
      "doi-asserted-by": "crossref",
      "first-page": "237",
      "DOI": "10.1016/0003-2697(89)90045-6",
      "article-title": "1-p-chlorophenyl-4,4-dimethyl-5-diethylamino-1-penten-3-one hydrobromide, a sulfhydryl-specific compound which reacts irreversibly with protein thiols but reversibly with small molecular weight thiols",
      "volume": "177",
      "author": "B Mutus",
      "year": "1989",
      "journal-title": "Anal Biochem"
    },
    {
      "key": "ref49",
      "first-page": "2053",
      "article-title": "Reactivity of some \u03b1, \u03b2-unsaturated ketones towards sulphydryl compounds and their antifungal activity",
      "volume": "1964",
      "author": "G Baluja",
      "year": "1964",
      "journal-title": "Chem Ind"
    },
    {
      "key": "ref50",
      "doi-asserted-by": "crossref",
      "first-page": "77",
      "DOI": "10.1016/j.jmb.2007.06.016",
      "article-title": "The closed MTIP-myosin A-tail complex from the malaria parasite invasion machinery",
      "volume": "372",
      "author": "J Bosch",
      "year": "2007",
      "journal-title": "J Mol Biol"
    },
    {
      "key": "ref51",
      "doi-asserted-by": "crossref",
      "first-page": "410",
      "DOI": "10.1016/j.chom.2011.09.004",
      "article-title": "The phosphoproteomes of <italic>Plasmodium falciparum</italic> and <italic>Toxoplasma gondii</italic> reveal unusual adaptations within and beyond the parasites&apos; boundaries",
      "volume": "10",
      "author": "M Treeck",
      "year": "2011",
      "journal-title": "Cell Host Microbe"
    }
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