DOI: 10.1366/0003702953964273. Use of Internal Reflectance Infrared Spectroscopy for the <i>in Situ</i> Study of Supported Lipid Monolayers [Penn]

Use of Internal Reflectance Infrared Spectroscopy for the in Situ Study of Supported Lipid Monolayers

10.1366/0003702953964273

Crossref journal-article

SAGE Publications

Applied Spectroscopy (179)

Abstract

An instrument has been developed to acquire polarized attenuated total internal reflection (ATIR) mid-IR spectra of supported monomolecular films in situ at the lipid/water (L/W) interface. The monolayer film is applied to the treated surface of a trapezoidal germanium crystal as it is immersed into the subphase of a specially designed Langmuir-Blodgett trough. This approach offers several advantages over the study of monolayer films at the air/water (A/W) interface by external reflection techniques, including (1) an improved signal throughput and multiple reflections, both contributing to an improved signal-to-noise (S/N) ratio; (2) versatility with respect to the chemical nature of the surface; and (3) the ability to use standard spectral subtraction methods to compensate for absorption of the aqueous subphase. The enhanced sensitivity of this approach facilitates the study of in situ interactions of peptides with fully hydrated lipid membrane surfaces.

Bibliography

Axelsen, P. H., Braddock, W. D., Brockman, H. L., Jones, C. M., Dluhy, R. A., Kaufman, B. K., & Puga, F. J. (1995). Use of Internal Reflectance Infrared Spectroscopy for the in Situ Study of Supported Lipid Monolayers. Applied Spectroscopy, 49(4), 526â531.

Authors 7

Paul H. Axelsen (first)
W. David Braddock (additional)
Howard L. Brockman (additional)
Craig M. Jones (additional)
Richard A. Dluhy (additional)
Brad K. Kaufman (additional)
Francisco J. Puga (additional)

References 25 Referenced 43

Swalen J. D. and Rabolt J. F., in Fourier Transform Infrared Spectroscopy: Application to Chemical Systems, Ferraro J. R. and Basile L. J., Eds. (Academic Press, New York, 1985), pp. 283–314. (10.1016/B978-0-12-254104-9.50013-4)
10.1021/j100398a033
10.1021/ac00125a023
10.1021/j100342a064
{'volume-title': 'Insoluble Monolayers at Liquid-Gas Interfaces', 'year': '1966', 'author': 'Gaines G. L.', 'key': 'bibr5-0003702953964273'} / Insoluble Monolayers at Liquid-Gas Interfaces by Gaines G. L. (1966)
{'volume-title': '“Fourier Transform Infrared Spectroscopy in Colloid and Interface Science”', 'year': '1991', 'author': 'Dluhy R. A.', 'key': 'bibr6-0003702953964273'} / “Fourier Transform Infrared Spectroscopy in Colloid and Interface Science” by Dluhy R. A. (1991)
10.1021/j100151a044
10.1063/1.465449
10.1016/S0006-3495(93)81276-1
10.1016/S0021-9258(18)99381-6
10.1007/978-94-010-9063-6_18
{'volume-title': 'Internal Reflection Spectroscopy', 'year': '1967', 'author': 'Harrick N. J.', 'key': 'bibr12-0003702953964273'} / Internal Reflection Spectroscopy by Harrick N. J. (1967)
10.1016/0021-9797(90)90025-J
10.1021/bi00399a020
10.1007/978-3-642-81537-9_6
10.1016/S0006-3495(91)82126-9
10.1029/JC080i012p01656
{'key': 'bibr18-0003702953964273', 'first-page': 'A65', 'volume': '66', 'author': 'Braddock W. D.', 'year': '1994', 'journal-title': 'Biophys. J.'} / Biophys. J. by Braddock W. D. (1994)
{'key': 'bibr19-0003702953964273', 'first-page': 'A62', 'volume': '64', 'author': 'Braddock W. D.', 'year': '1993', 'journal-title': 'Biophys. J.'} / Biophys. J. by Braddock W. D. (1993)
10.1016/0005-2736(83)90523-0
10.1021/bi00240a026
10.1016/S0021-9258(18)68909-4 / J. Biol. Chem. by Batenburg A. M. (1988)
10.1016/0005-2736(93)90210-Q
10.1016/0005-2736(93)90389-H
{'key': 'bibr25-0003702953964273', 'first-page': 'A373', 'volume': '66', 'author': 'Reisdorf W. C.', 'year': '1994', 'journal-title': 'Biophys. J.'} / Biophys. J. by Reisdorf W. C. (1994)

Dates

Type	When
Created	20 years, 3 months ago (May 21, 2005, 12:24 p.m.)
Deposited	5 months, 2 weeks ago (March 10, 2025, 5:16 p.m.)
Indexed	5 months, 2 weeks ago (March 11, 2025, 12:34 a.m.)
Issued	30 years, 4 months ago (April 1, 1995)
Published	30 years, 4 months ago (April 1, 1995)
Published Online	30 years, 4 months ago (April 1, 1995)
Published Print	30 years, 4 months ago (April 1, 1995)

Funders 0

None

BibTeX

@article{Axelsen_1995, title={Use of Internal Reflectance Infrared Spectroscopy for the in Situ Study of Supported Lipid Monolayers}, volume={49}, ISSN={1943-3530}, url={http://dx.doi.org/10.1366/0003702953964273}, DOI={10.1366/0003702953964273}, number={4}, journal={Applied Spectroscopy}, publisher={SAGE Publications}, author={Axelsen, Paul H. and Braddock, W. David and Brockman, Howard L. and Jones, Craig M. and Dluhy, Richard A. and Kaufman, Brad K. and Puga, Francisco J.}, year={1995}, month=apr, pages={526–531} }

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