DOI: 10.1366/000370202321115977. Surface-Enhanced Resonance Raman Spectroscopy Signals from Single Myoglobin Molecules

Surface-Enhanced Resonance Raman Spectroscopy Signals from Single Myoglobin Molecules

10.1366/000370202321115977

Crossref journal-article

SAGE Publications

Applied Spectroscopy (179)

Abstract

The extremely large cross-section available from metallic surface enhancement has been exploited to investigate the Raman spectrum of heme myoglobin adsorbed on silver colloidal nanoparticles at very low concentrations. The study has been performed on particles both in solution and immobilized onto a polymer-coated glass surface. In both the cases, we have observed striking temporal fluctuations in the surface-enhanced resonance Raman spectroscopy (SERRS) spectra collected at short times. A statistical analysis of the temporal intensity fluctuations and of the associated correlations of the Raman signals has allowed us to verify that the single molecule limit is approached. The possible connections of these fluctuations with the entanglement of the biomolecule within the local minima of its rough energy landscape is discussed.

Bibliography

Bizzarri, A. R., & Cannistraro, S. (2002). Surface-Enhanced Resonance Raman Spectroscopy Signals from Single Myoglobin Molecules. Applied Spectroscopy, 56(12), 1531â1537.

Authors 2

Anna Rita Bizzarri (first)
Salvatore Cannistraro (additional)

References 33 Referenced 79

10.1021/cr980133r
10.1126/science.275.5303.1102
10.1103/PhysRevLett.83.4357
{'volume-title': 'Single-Molecule Optical Detection, Imaging and Spectroscopy', 'year': '1997', 'author': "Basche' T.", 'key': 'bibr4-000370202321115977'} / Single-Molecule Optical Detection, Imaging and Spectroscopy by Basche' T. (1997)
10.1063/1.481385
10.1017/CBO9781139167864
10.1002/9780470141205
10.1146/annurev.bb.17.060188.002315
10.1103/PhysRevLett.57.1267
10.1007/BF00180260
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{'key': 'bibr16-000370202321115977', 'first-page': '898', 'volume': '289', 'author': 'Yu J.', 'year': '2001', 'journal-title': 'Science (Washington, D.C.)'} / Science (Washington, D.C.) by Yu J. (2001)
{'volume-title': 'Molecular Electronic–Science and Technology', 'year': '1992', 'author': 'Aviram A.', 'key': 'bibr17-000370202321115977'} / Molecular Electronic–Science and Technology by Aviram A. (1992)
10.1146/annurev.pc.43.100192.003443
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10.1103/RevModPhys.57.783
10.1021/ja992128q
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10.1103/PhysRevLett.78.1667
10.1021/jp0025476
10.1021/jp002552+
10.1021/jp012268y
Antonini E., Brunori M., in Hemoglobin and Myoglobin in their Reactions with Ligands (North Holland Publ. Co., London, 1995), Vol. 27, p. 241; Stryer L., in Biochemistry (Freeman, New York, 1995), 4th ed.; Sage J. T., Champion P. M., in Comprehensive Supramolecular Chemistry, Suslick K. S. Ed. (Pergamon, Oxford, UK, 1996), Vol. 5, p. 171; Rovira C., Schulze B., Eichinger M., Evanseck J. D., Parrinello M., Biophys. J. 81, 435 (2001), and references therein.
10.1016/S0009-2614(01)01245-3
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10.1021/ja962239e

Dates

Type	When
Created	21 years, 10 months ago (Oct. 17, 2003, 12:32 p.m.)
Deposited	5 months, 1 week ago (March 11, 2025, 2:32 a.m.)
Indexed	5 months, 1 week ago (March 12, 2025, 12:20 a.m.)
Issued	22 years, 8 months ago (Dec. 1, 2002)
Published	22 years, 8 months ago (Dec. 1, 2002)
Published Online	22 years, 8 months ago (Dec. 1, 2002)
Published Print	22 years, 8 months ago (Dec. 1, 2002)

Funders 0

None

BibTeX

@article{Bizzarri_2002, title={Surface-Enhanced Resonance Raman Spectroscopy Signals from Single Myoglobin Molecules}, volume={56}, ISSN={1943-3530}, url={http://dx.doi.org/10.1366/000370202321115977}, DOI={10.1366/000370202321115977}, number={12}, journal={Applied Spectroscopy}, publisher={SAGE Publications}, author={Bizzarri, Anna Rita and Cannistraro, Salvatore}, year={2002}, month=dec, pages={1531–1537} }

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