DOI: 10.1366/0003702011953018. Near-Infrared Raman Spectroscopy for <i>in vivo</i> Detection of Cervical Precancers

Near-Infrared Raman Spectroscopy for in vivo Detection of Cervical Precancers

10.1366/0003702011953018

Crossref journal-article

SAGE Publications

Applied Spectroscopy (179)

Abstract

This study evaluates the potential of near-infrared Raman spectroscopy for in vivo detection of squamous dysplasia, a precursor to cervical cancer. A pilot clinical trial was carried out at three clinical sites. Raman spectra were measured from one colposcopically normal and one abnormal area of the cervix. These sites were then biopsied and submitted for routine histologic analysis. Twentyfour evaluable measurements were made in vivo in 13 patients. Cervical tissue Raman spectra contain peaks in the vicinity of 1070, 1180, 1195, 1210, 1245, 1330, 1400, 1454, 1505, 1555, 1656, and 1760 cm−1. The ratio of intensities at 1454 to 1656 cm−1 is greater for squamous dysplasia than all other tissue types, while the ratio of intensities at 1330 to 1454 cm−1 is lower for samples with squamous dysplasia than all other tissue types. A simple algorithm based on these two intensity ratios separates high-grade squamous dysplasia from all others, misclassifying only one sample. Spectra measured in vivo resemble those measured in vitro. Cervical epithelial cells may contribute to tissue spectra at 1330 cm−1, a region associated with DNA. In contrast, epithelial cells probably do not contribute to tissue spectra at 1454 cm−1, a region associated with collagen and phospholipids.

Bibliography

Utzinger, U., Heintzelman, D. L., Mahadevan-Jansen, A., Malpica, A., Follen, M., & Richards-Kortum, R. (2001). Near-Infrared Raman Spectroscopy for in vivo Detection of Cervical Precancers. Applied Spectroscopy, 55(8), 955â959.

Authors 6

Urs Utzinger (first)
Douglas L. Heintzelman (additional)
Anita Mahadevan-Jansen (additional)
Anais Malpica (additional)
Michele Follen (additional)
Rebecca Richards-Kortum (additional)

References 25 Referenced 211

10.1161/01.CIR.97.9.878
10.1109/10.133205
10.1562/0031-8655(2000)072<0103:OEWFIV>2.0.CO;2
10.1016/S0016-5107(98)70009-4
10.1111/j.1751-1097.1996.tb03130.x
10.1067/mob.2000.104844
{'volume-title': 'M.S. Thesis', 'year': '1998', 'author': 'Zuluaga A.', 'key': 'bibr7-0003702011953018'} / M.S. Thesis by Zuluaga A. (1998)
10.1126/science.3287615
{'key': 'bibr9-0003702011953018', 'first-page': '462', 'volume': '93', 'author': 'Mitchell M. F.', 'year': '1999', 'journal-title': 'Obstet. Gynecol.'} / Obstet. Gynecol. by Mitchell M. F. (1999)
10.1088/0031-9155/45/2/201
10.1117/12.227815
10.1111/j.1751-1097.1998.tb05160.x
10.1021/ac00101a001
10.1111/j.1751-1097.1998.tb03262.x
10.1046/j.1525-1594.2000.06525.x
10.1111/j.1751-1097.1998.tb09703.x
10.1366/0003702991947225
10.1366/0003702963905592
10.1366/0003702963905574
10.6028/jres.097.002
10.1109/78.678493
10.1021/ac60214a047
10.1366/0003702991947829
10.1109/10.797989
{'key': 'bibr25-0003702011953018', 'first-page': '146', 'volume': '72', 'author': 'Shim M. G.', 'year': '2000', 'journal-title': 'Photochem. Photobiol.'} / Photochem. Photobiol. by Shim M. G. (2000)

Dates

Type	When
Created	21 years, 10 months ago (Oct. 17, 2003, 12:32 p.m.)
Deposited	5 months, 2 weeks ago (March 10, 2025, 12:16 p.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 8:27 a.m.)
Issued	24 years ago (Aug. 1, 2001)
Published	24 years ago (Aug. 1, 2001)
Published Online	24 years ago (Aug. 1, 2001)
Published Print	24 years ago (Aug. 1, 2001)

Funders 0

None

BibTeX

@article{Utzinger_2001, title={Near-Infrared Raman Spectroscopy for in vivo Detection of Cervical Precancers}, volume={55}, ISSN={1943-3530}, url={http://dx.doi.org/10.1366/0003702011953018}, DOI={10.1366/0003702011953018}, number={8}, journal={Applied Spectroscopy}, publisher={SAGE Publications}, author={Utzinger, Urs and Heintzelman, Douglas L. and Mahadevan-Jansen, Anita and Malpica, Anais and Follen, Michele and Richards-Kortum, Rebecca}, year={2001}, month=aug, pages={955–959} }

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  "abstract": "<jats:p> This study evaluates the potential of near-infrared Raman spectroscopy for in vivo detection of squamous dysplasia, a precursor to cervical cancer. A pilot clinical trial was carried out at three clinical sites. Raman spectra were measured from one colposcopically normal and one abnormal area of the cervix. These sites were then biopsied and submitted for routine histologic analysis. Twentyfour evaluable measurements were made in vivo in 13 patients. Cervical tissue Raman spectra contain peaks in the vicinity of 1070, 1180, 1195, 1210, 1245, 1330, 1400, 1454, 1505, 1555, 1656, and 1760 cm<jats:sup>\u22121</jats:sup>. The ratio of intensities at 1454 to 1656 cm<jats:sup>\u22121</jats:sup> is greater for squamous dysplasia than all other tissue types, while the ratio of intensities at 1330 to 1454 cm<jats:sup>\u22121</jats:sup> is lower for samples with squamous dysplasia than all other tissue types. A simple algorithm based on these two intensity ratios separates high-grade squamous dysplasia from all others, misclassifying only one sample. Spectra measured in vivo resemble those measured in vitro. Cervical epithelial cells may contribute to tissue spectra at 1330 cm<jats:sup>\u22121</jats:sup>, a region associated with DNA. In contrast, epithelial cells probably do not contribute to tissue spectra at 1454 cm<jats:sup>\u22121</jats:sup>, a region associated with collagen and phospholipids. </jats:p>",
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