DOI: 10.1359/jbmr.1997.12.12.2040. Gene Expression Patterns of Murine Dentin Matrix Protein 1 (Dmp1) and Dentin Sialophosphoprotein (DSPP) Suggest Distinct Developmental Functions In Vivo

Gene Expression Patterns of Murine Dentin Matrix Protein 1 (Dmp1) and Dentin Sialophosphoprotein (DSPP) Suggest Distinct Developmental Functions In Vivo

10.1359/jbmr.1997.12.12.2040

Crossref journal-article

Oxford University Press (OUP)

Journal of Bone and Mineral Research (286)

Abstract

Abstract Although the precise mechanisms of the conversion of predentin to dentin are not well understood, several lines of evidence implicate the noncollagenous proteins (NCPs) as important regulators of dentin biomineralization. Here we compared the in vivo temporospatial expression patterns of two dentin NCP genes, dentin matrix protein 1 (Dmp1), and dentin sialophosphoprotein (DSPP) in developing molars. Reverse transcription-polymerase chain reaction was performed on embryonic day 13 to 1-day-old first molars using Dmp1- and DSPP-specific primer sets. Dmp1 transcripts appeared at the late bud stage, while DSPP mRNA was seen at the cap stage. Expression of both genes was sustained throughout odontogenesis. In situ hybridization analysis revealed interesting differences in the expression patterns of these genes. While Dmp1 and DSPP showed coexpression in young odontoblasts before the start of mineralization, the expression of these genes was notably distinct at later stages. Dmp1 expression decreased in secretory odontoblasts after the appearance of mineral, while high levels of DSPP were sustained in odontoblasts. In early secretory ameloblasts, DSPP expression was transient and down-regulated with the appearance of dentin matrix. Interestingly, Dmp1 expression became evident in ameloblasts during the maturative phase of amelogenesis. In contrast to Dspp expression that was tooth-specific, Dmp1 was expressed by osteoblasts throughout ossification in the skeleton. Probes directed to the “DSP” and “DPP” regions of the DSPP gene showed identical patterns of mRNA expression. These data show that the developmental expression patterns of Dmp1 and DSPP are distinct, implying that these molecules serve different biological functions in vivo.

Bibliography

Dâsouza, R. N., Cavender, A., Sunavala, G., Alvarez, J., Ohshima, T., Kulkarni, A. B., & MacDougall, M. (1997). Gene Expression Patterns of Murine Dentin Matrix Protein 1 (Dmp1) and Dentin Sialophosphoprotein (DSPP) Suggest Distinct Developmental Functions In Vivo. Journal of Bone and Mineral Research, 12(12), 2040â2049.

Authors 7

R. N. D'souza (first)
A. Cavender (additional)
G. Sunavala (additional)
J. Alvarez (additional)
T. Ohshima (additional)
A. B. Kulkarni (additional)
M. MacDougall (additional)

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Dates

Type	When
Created	19 years, 4 months ago (April 26, 2006, 3:44 p.m.)
Deposited	1 year, 5 months ago (March 13, 2024, 10:54 a.m.)
Indexed	1 week, 4 days ago (Aug. 26, 2025, 3:03 a.m.)
Issued	27 years, 9 months ago (Dec. 1, 1997)
Published	27 years, 9 months ago (Dec. 1, 1997)
Published Online	15 years, 9 months ago (Dec. 4, 2009)
Published Print	27 years, 9 months ago (Dec. 1, 1997)

Funders 0

None

BibTeX

@article{D_souza_1997, title={Gene Expression Patterns of Murine Dentin Matrix Protein 1 (Dmp1) and Dentin Sialophosphoprotein (DSPP) Suggest Distinct Developmental Functions In Vivo}, volume={12}, ISSN={1523-4681}, url={http://dx.doi.org/10.1359/jbmr.1997.12.12.2040}, DOI={10.1359/jbmr.1997.12.12.2040}, number={12}, journal={Journal of Bone and Mineral Research}, publisher={Oxford University Press (OUP)}, author={D’souza, R. N. and Cavender, A. and Sunavala, G. and Alvarez, J. and Ohshima, T. and Kulkarni, A. B. and MacDougall, M.}, year={1997}, month=dec, pages={2040–2049} }

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  "abstract": "<jats:title>Abstract</jats:title>\n               <jats:p>Although the precise mechanisms of the conversion of predentin to dentin are not well understood, several lines of evidence implicate the noncollagenous proteins (NCPs) as important regulators of dentin biomineralization. Here we compared the in vivo temporospatial expression patterns of two dentin NCP genes, dentin matrix protein 1 (Dmp1), and dentin sialophosphoprotein (DSPP) in developing molars. Reverse transcription-polymerase chain reaction was performed on embryonic day 13 to 1-day-old first molars using Dmp1- and DSPP-specific primer sets. Dmp1 transcripts appeared at the late bud stage, while DSPP mRNA was seen at the cap stage. Expression of both genes was sustained throughout odontogenesis. In situ hybridization analysis revealed interesting differences in the expression patterns of these genes. While Dmp1 and DSPP showed coexpression in young odontoblasts before the start of mineralization, the expression of these genes was notably distinct at later stages. Dmp1 expression decreased in secretory odontoblasts after the appearance of mineral, while high levels of DSPP were sustained in odontoblasts. In early secretory ameloblasts, DSPP expression was transient and down-regulated with the appearance of dentin matrix. Interestingly, Dmp1 expression became evident in ameloblasts during the maturative phase of amelogenesis. In contrast to Dspp expression that was tooth-specific, Dmp1 was expressed by osteoblasts throughout ossification in the skeleton. Probes directed to the \u201cDSP\u201d and \u201cDPP\u201d regions of the DSPP gene showed identical patterns of mRNA expression. These data show that the developmental expression patterns of Dmp1 and DSPP are distinct, implying that these molecules serve different biological functions in vivo.</jats:p>",
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