DOI: 10.1261/rna.7320604. The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome

The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome

10.1261/rna.7320604

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

U11 and U12 snRNPs bind U12-type pre-mRNAs as a preformed di-snRNP complex, simultaneously recognizing the 5′ splice site and branchpoint sequence. Thus, within the U12-type prespliceosome, U11/U12 components form a molecular bridge connecting both ends of the intron. We have affinity purified human 18S U11/U12 and 12S U11 snRNPs, and identified their protein components by using mass spectrometry. U11/U12 snRNPs lack all known U1 snRNP proteins but contain seven novel proteins (i.e., 65K, 59K, 48K, 35K, 31K, 25K, 20K) not found in the major spliceosome, four of which (59K, 48K, 35K, and 25K) are U11-associated. Thus, protein–protein and protein–RNA interactions contributing to 5′ splice site recognition and/or intron bridging appear to differ significantly in the minor versus major prespliceosome. The majority of U11/U12 proteins are highly conserved in organisms known to contain U12-type introns. However, homologs of those associated with U11 were not detected inDrosophila melanogaster, consistent with the presence of a divergent U11 snRNP in flies. RNAi experiments revealed that several U11/U12 proteins are essential for cell viability, suggesting they play key roles in U12-type splicing. The presence of unique U11/U12 snRNP proteins in the U12-type spliceosome provides insight into potential evolutionary relationships between the major and minor spliceosome.

Bibliography

WILL, C. L., SCHNEIDER, C., HOSSBACH, M., URLAUB, H., RAUHUT, R., ELBASHIR, S., TUSCHL, T., & LÃHRMANN, R. (2004). The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA, 10(6), 929â941.

Authors 8

CINDY L. WILL (first)
CLAUDIA SCHNEIDER (additional)
MARKUS HOSSBACH (additional)
HENNING URLAUB (additional)
REINHARD RAUHUT (additional)
SAYDA ELBASHIR (additional)
THOMAS TUSCHL (additional)
REINHARD LÜHRMANN (additional)

References 39 Referenced 133

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Dates

Type	When
Created	21 years, 3 months ago (May 14, 2004, 1:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 6:41 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 7, 2025, 5:26 p.m.)
Issued	21 years, 3 months ago (May 14, 2004)
Published	21 years, 3 months ago (May 14, 2004)
Published Online	21 years, 3 months ago (May 14, 2004)
Published Print	21 years, 2 months ago (June 1, 2004)

Funders 0

None

BibTeX

@article{WILL_2004, title={The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome}, volume={10}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.7320604}, DOI={10.1261/rna.7320604}, number={6}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={WILL, CINDY L. and SCHNEIDER, CLAUDIA and HOSSBACH, MARKUS and URLAUB, HENNING and RAUHUT, REINHARD and ELBASHIR, SAYDA and TUSCHL, THOMAS and LÜHRMANN, REINHARD}, year={2004}, month=may, pages={929–941} }

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  "abstract": "<jats:p>U11 and U12 snRNPs bind U12-type pre-mRNAs as a preformed di-snRNP complex, simultaneously recognizing the 5\u2032 splice site and branchpoint sequence. Thus, within the U12-type prespliceosome, U11/U12 components form a molecular bridge connecting both ends of the intron. We have affinity purified human 18S U11/U12 and 12S U11 snRNPs, and identified their protein components by using mass spectrometry. U11/U12 snRNPs lack all known U1 snRNP proteins but contain seven novel proteins (i.e., 65K, 59K, 48K, 35K, 31K, 25K, 20K) not found in the major spliceosome, four of which (59K, 48K, 35K, and 25K) are U11-associated. Thus, protein\u2013protein and protein\u2013RNA interactions contributing to 5\u2032 splice site recognition and/or intron bridging appear to differ significantly in the minor versus major prespliceosome. The majority of U11/U12 proteins are highly conserved in organisms known to contain U12-type introns. However, homologs of those associated with U11 were not detected in<jats:italic>Drosophila melanogaster</jats:italic>, consistent with the presence of a divergent U11 snRNP in flies. RNAi experiments revealed that several U11/U12 proteins are essential for cell viability, suggesting they play key roles in U12-type splicing. The presence of unique U11/U12 snRNP proteins in the U12-type spliceosome provides insight into potential evolutionary relationships between the major and minor spliceosome.</jats:p>",
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