DOI: 10.1261/rna.688207. Roles of the negatively charged N-terminal extension of <i>Saccharomyces cerevisiae</i> ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5

Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5

10.1261/rna.688207

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

Ribosomal protein (rp) S5 belongs to a family of ribosomal proteins that includes bacterial rpS7. rpS5 forms part of the exit (E) site on the 40S ribosomal subunit and is essential for yeast viability. Human rpS5 is 67% identical and 79% similar to Saccharomyces cerevisiae rpS5 but lacks a negatively charged (pI ∼3.27) 21 amino acid long N-terminal extension that is present in fungi. Here we report that replacement of yeast rpS5 with its human homolog yielded a viable yeast strain with a 20%–25% decrease in growth rate. This replacement also resulted in a moderate increase in the heavy polyribosomal components in the mutant strain, suggesting either translation elongation or termination defects, and in a reduction in the polyribosomal association of the elongation factors eEF3 and eEF1A. In addition, the mutant strain was characterized by moderate increases in +1 and −1 programmed frameshifting and hyperaccurate recognition of the UAA stop codon. The activities of the cricket paralysis virus (CrPV) IRES and two mammalian cellular IRESs (CAT-1 and SNAT-2) were also increased in the mutant strain. Consistently, the rpS5 replacement led to enhanced direct interaction between the CrPV IRES and the mutant yeast ribosomes. Taken together, these data indicate that rpS5 plays an important role in maintaining the accuracy of translation in eukaryotes and suggest that the negatively charged N-terminal extension of yeast rpS5 might affect the ribosomal recruitment of specific mRNAs.

Bibliography

Galkin, O., Bentley, A. A., Gupta, S., Compton, B.-A., Mazumder, B., Kinzy, T. G., Merrick, W. C., Hatzoglou, M., Pestova, T. V., Hellen, C. U. T., & Komar, A. A. (2007). Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5. RNA, 13(12), 2116â2128.

Authors 11

Oleksandr Galkin (first)
Amber A. Bentley (additional)
Sujatha Gupta (additional)
Beth-Ann Compton (additional)
Barsanjit Mazumder (additional)
Terri Goss Kinzy (additional)
William C. Merrick (additional)
Maria Hatzoglou (additional)
Tatyana V. Pestova (additional)
Christopher U.T. Hellen (additional)
Anton A. Komar (additional)

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Dates

Type	When
Created	17 years, 10 months ago (Sept. 27, 2007, 8:39 p.m.)
Deposited	3 years, 9 months ago (Nov. 21, 2021, 8:52 a.m.)
Indexed	5 months ago (March 22, 2025, 5:04 a.m.)
Issued	17 years, 10 months ago (Sept. 27, 2007)
Published	17 years, 10 months ago (Sept. 27, 2007)
Published Online	17 years, 10 months ago (Sept. 27, 2007)
Published Print	17 years, 8 months ago (Dec. 1, 2007)

Funders 0

None

BibTeX

@article{Galkin_2007, title={Roles of the negatively charged N-terminal extension of Saccharomyces cerevisiae ribosomal protein S5 revealed by characterization of a yeast strain containing human ribosomal protein S5}, volume={13}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.688207}, DOI={10.1261/rna.688207}, number={12}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={Galkin, Oleksandr and Bentley, Amber A. and Gupta, Sujatha and Compton, Beth-Ann and Mazumder, Barsanjit and Kinzy, Terri Goss and Merrick, William C. and Hatzoglou, Maria and Pestova, Tatyana V. and Hellen, Christopher U.T. and Komar, Anton A.}, year={2007}, month=sep, pages={2116–2128} }

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