DOI: 10.1261/rna.61206. Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits

Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits

10.1261/rna.61206

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

GE82832, a secondary metabolite produced by Streptosporangium cinnabarinum (strain GE82832), has been identified as a translational inhibitor by in vitro screening of a library of natural products. Secondary functional tests specific for individual steps of the translational pathway demonstrated that translocation is the specific target of GE82832. Chemical probing in situ demonstrated that this antibiotic protects bases A1324 and A1333 and exposes C1336 of 16S rRNA, thereby indicating that its binding site is located on the head of the 30S ribosomal subunit. The ribosomal location of GE82832, near ribosomal protein S13 and G1338, two elements of the small subunit that are part of or close to the B1a intrasubunit bridge, suggests that translocation inhibition results from an altered dynamics of 30S–50S ribosomal subunit interaction.

Bibliography

Brandi, L., Fabbretti, A., Stefano, M. D., Lazzarini, A., Abbondi, M., & Gualerzi, C. O. (2006). Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits. RNA, 12(7), 1262â1270.

Authors 6

Letizia Brandi (first)
Attilio Fabbretti (additional)
Michele Di Stefano (additional)
Ameriga Lazzarini (additional)
Monica Abbondi (additional)
Claudio O. Gualerzi (additional)

References 35 Referenced 28

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{'key': '2021112105410220000_12.7.1262.18', 'first-page': '13', 'article-title': 'Dinucleotide codon–anticodon interaction as a minimum requirement for ribosomal aa-tRNA binding: Stabilisation by viomycin of aa-tRNA in the A site', 'volume': '58', 'author': 'Luehrmann', 'year': '1980', 'journal-title': 'Nucleic Acids Res.'} / Nucleic Acids Res. / Dinucleotide codon–anticodon interaction as a minimum requirement for ribosomal aa-tRNA binding: Stabilisation by viomycin of aa-tRNA in the A site by Luehrmann (1980)
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Dates

Type	When
Created	19 years, 3 months ago (May 12, 2006, 9:18 p.m.)
Deposited	3 years, 9 months ago (Nov. 21, 2021, 8:46 a.m.)
Indexed	3 months, 3 weeks ago (May 6, 2025, 9:27 a.m.)
Issued	19 years, 3 months ago (May 12, 2006)
Published	19 years, 3 months ago (May 12, 2006)
Published Online	19 years, 3 months ago (May 12, 2006)
Published Print	19 years, 2 months ago (July 1, 2006)

Funders 0

None

BibTeX

@article{Brandi_2006, title={Characterization of GE82832, a peptide inhibitor of translocation interacting with bacterial 30S ribosomal subunits}, volume={12}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.61206}, DOI={10.1261/rna.61206}, number={7}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={Brandi, Letizia and Fabbretti, Attilio and Stefano, Michele Di and Lazzarini, Ameriga and Abbondi, Monica and Gualerzi, Claudio O.}, year={2006}, month=may, pages={1262–1270} }

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