DOI: 10.1261/rna.5810203. The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies: FIGURE 1.

The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies: FIGURE 1.

10.1261/rna.5810203

Crossref journal-article

Cold Spring Harbor Laboratory

RNA (246)

Abstract

A novel cytoplasmic compartment referred to as GW bodies (GWBs) was initially identified using antibodies specific to a 182-kD protein termed GW182. GW182 was characterized by multiple glycine(G)-tryptophan(W) repeats and an RNA recognition motif (RRM) that bound a subset of HeLa cell messenger RNAs (mRNAs). The function of GWBs was not known; however, more recent evidence suggested similarities between GWBs and cytoplasmic structures that contain hLSm proteins and hDcp1, the human homolog to a yeast decapping enzyme subunit. In this study, we used antibodies to hLSm4 and hDcp1 to show that both of these markers of an mRNA degradation pathway colocalize to the same structures as GW182. Our studies demonstrate that GW182, hLSm4, and hDcp1 are found in the same cytoplasmic structures and suggest that GW182 is involved in the same mRNA processing pathway as hLSm4 and hDcp1.

Bibliography

EYSTATHIOY, T., JAKYMIW, A., CHAN, E. K. L., SÃRAPHIN, B., COUGOT, N., & FRITZLER, M. J. (2003). The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies: FIGURE 1. RNA, 9(10), 1171â1173.

Authors 6

THEOPHANY EYSTATHIOY (first)
ANDREW JAKYMIW (additional)
EDWARD K.L. CHAN (additional)
BERTRAND SÉRAPHIN (additional)
NICOLAS COUGOT (additional)
MARVIN J. FRITZLER (additional)

References 24 Referenced 212

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Dates

Type	When
Created	21 years, 11 months ago (Sept. 16, 2003, 4:27 p.m.)
Deposited	2 years, 4 months ago (April 26, 2023, 6:58 p.m.)
Indexed	5 days, 9 hours ago (Aug. 23, 2025, 9:31 p.m.)
Issued	21 years, 11 months ago (Sept. 16, 2003)
Published	21 years, 11 months ago (Sept. 16, 2003)
Published Online	21 years, 11 months ago (Sept. 16, 2003)
Published Print	21 years, 10 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{EYSTATHIOY_2003, title={The GW182 protein colocalizes with mRNA degradation associated proteins hDcp1 and hLSm4 in cytoplasmic GW bodies: FIGURE 1.}, volume={9}, ISSN={1469-9001}, url={http://dx.doi.org/10.1261/rna.5810203}, DOI={10.1261/rna.5810203}, number={10}, journal={RNA}, publisher={Cold Spring Harbor Laboratory}, author={EYSTATHIOY, THEOPHANY and JAKYMIW, ANDREW and CHAN, EDWARD K.L. and SÉRAPHIN, BERTRAND and COUGOT, NICOLAS and FRITZLER, MARVIN J.}, year={2003}, month=sep, pages={1171–1173} }

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